Filogenia do gênero Spondias com base em marcadores RAPD resultados preliminares.

O trabalho teve como objetivo, estudar as relações de ancestralidade (filogenia) entre as diferentes Spondias visando agrupar as espécies/híbridos com base em marcadores de DNA do tipo RAPD

A HPLC‐DAD method for identifying and estimating the content of fucoxanthin, β‐carotene and chlorophyll a in brown algal extracts

Seaweeds are photosynthetic organisms that have high contents of pigments. The coloration of each alga is defined by the content and combination of pigments synthesized, which varies among species and environmental conditions. The most abundant pigments in algae are chlorophylls and carotenoids, lipophilic molecules that can be used as natural colorants and have high acceptance by consumers. In this work, a simple and short hands-on time HPLC-DAD method for identifying and estimating the pigment content of algal extracts, specifically fucoxanthin, β-carotene and chlorophyll a was carried out. Using this optimized method, a pigment screening was performed on the ethanolic extracts obtained by ultrasound-assisted extraction from nine brown algal from the Atlantic coastline: Ascophyllum nodosum, Bifurcaria bifurcata, Fucus spiralis, Himanthalia elongata, Laminaria saccharina, Laminaria ochroleuca, Pelvetia canaliculata, Sargassum muticum and Undaria pinnatifida. HPLC results permitted to highlight L. saccharina and U. pinnatifida as promising sources of these three target pigments containing a total amount of 10.5 – 11.5 mg per gram of dry weight. Among them, the most abundant one was fucoxanthin, an added-value compound with a high potential to be commercially exploited by different industries, such as the food, cosmetic, and pharmaceutical sectors.The research leading to these results was supported by MICINN sup- porting the Ramón y Cajal grant for M.A. Prieto (RYC-2017-22891), the FPU grant for A. Carreira-Casais (FPU2016/06135); and by Xunta de Galicia for supporting the post-doctoral grant of M. Fraga-Corral (ED481B-2019/096). The research leading to these results was sup- ported by the European Union through the “NextGenerationEU ”pro- gram supporting the “Margarita Salas ”grant awarded to P. Garcia- Perez. Authors are grateful to AlgaMar company ( www.algamar.com ) for the collaboration and algal material provision. This research was funded by the Ibero-American Program on Science and Technology (CYTED —AQUA-CIBUS, P317RT0003), the Bio Based Industries Joint Undertaking (JU) under grant agreement No 888003 UP4HEALTH Project (H2020-BBI-JTI-2019) that supports the work of C. Lourenço- Lopes. The JU receives support from the European Union’s Horizon 2020 research and innovation program and the Bio Based Industries Consor- tium. The project SYSTEMIC Knowledge hub on Nutrition and Food Se- curity, has received funding from national research funding parties in Belgium (FWO), France (INRA), Germany (BLE), Italy (MIPAAF), Latvia (IZM), Norway (RCN), Portugal (FCT), and Spain (AEI) in a joint ac- tion of JPI HDHL, JPI-OCEANS and FACCE-JPI launched in 2019 un- der the ERA-NET ERA-HDHL (n°696295). The authors would like to thank the EU and FCT for funding through the project PTDC/OCE- ETA/30240/2017- SilverBrain - From sea to brain: Green neuropro- tective extracts for nanoencapsulation and functional food production (POCI-01-0145-FEDER-030240).info:eu-repo/semantics/publishedVersio

1281O Atezolizumab (atezo) vs platinum-based chemo in blood-based tumour mutational burden-positive (bTMB+) patients (pts) with first-line (1L) advanced/metastatic (m)NSCLC: Results of the Blood First Assay Screening Trial (BFAST) phase III cohort C

Background: TMB is a promising biomarker for immunotherapy in NSCLC, but current data are mostly retrospective. As not all pts may have sufficient tissue for comprehensive biomarker testing, bTMB was prospectively tested as a novel biomarker using targeted next-generation sequencing. BFAST (NCT03178552), a global, open-label, multi-cohort trial, evaluated safety and efficacy of targeted therapies or immunotherapy in biomarker-selected pts with unresectable mNSCLC. Here we present results from Cohort C of 1L atezo vs platinum-based chemo in pts with bTMB+ mNSCLC. Methods: We planned to randomise ≈440 pts with 1L mNSCLC with measurable disease per RECIST 1.1 and bTMB ≥10 (9.1 mut/Mb; FMI bTMB assay) 1:1 to atezo 1200 mg IV every 3 weeks or chemo and stratified by tissue availability, ECOG PS, bTMB and histology. The primary endpoint was INV-PFS per RECIST 1.1 in bTMB ≥16 (14.5 mut/Mb) pts. Key secondary endpoints included OS in bTMB ≥10 (intent to treat, ITT) and bTMB ≥16 pts, and INV-PFS in ITT pts. Results: 471 pts were assigned to atezo (n=234) or chemo (n=237). At baseline, 72% had non-squamous histology, 2% never smoked and median SLD was 103 mm. 145 pts with bTMB ≥16 were assigned to atezo and 146 to chemo. At data cutoff (21 May 2020) minimum follow up was 6 mo. INV-PFS difference in bTMB ≥16 pts for atezo vs chemo was not significant (P=0.053; Table). Grade 3-4 TRAEs occurred in 18% (atezo) vs 46% (chemo) of pts. Serious TRAEs occurred in 12% (atezo) vs 14% (chemo). Results at other bTMB thresholds and by F1L CDx will also be presented as an exploratory analysis. Conclusions: The primary PFS endpoint in bTMB ≥16 pts was not met. OS was numerically better with atezo vs chemo but the difference was not statistically significant. The safety profile of atezo vs chemo was favourable and consistent with atezo monotherapy across indications

NADPH Oxidase 5 Is a Pro‐Contractile Nox Isoform and a Point of Cross‐Talk for Calcium and Redox Signaling‐Implications in Vascular Function

Background NADPH Oxidase 5 (Nox5) is a calcium‐sensitive superoxide‐generating Nox. It is present in lower forms and higher mammals, but not in rodents. Nox5 is expressed in vascular cells, but the functional significance remains elusive. Given that contraction is controlled by calcium and reactive oxygen species, both associated with Nox5, we questioned the role of Nox5 in pro‐contractile signaling and vascular function. Methods and Results Transgenic mice expressing human Nox5 in a vascular smooth muscle cell–specific manner (Nox5 mice) and Rhodnius prolixus, an arthropod model that expresses Nox5 endogenoulsy, were studied. Reactive oxygen species generation was increased systemically and in the vasculature and heart in Nox5 mice. In Nox5‐expressing mice, agonist‐induced vasoconstriction was exaggerated and endothelium‐dependent vasorelaxation was impaired. Vascular structural and mechanical properties were not influenced by Nox5. Vascular contractile responses in Nox5 mice were normalized by N‐acetylcysteine and inhibitors of calcium channels, calmodulin, and endoplasmic reticulum ryanodine receptors, but not by GKT137831 (Nox1/4 inhibitor). At the cellular level, vascular changes in Nox5 mice were associated with increased vascular smooth muscle cell [Ca2+]i, increased reactive oxygen species and nitrotyrosine levels, and hyperphosphorylation of pro‐contractile signaling molecules MLC20 (myosin light chain 20) and MYPT1 (myosin phosphatase target subunit 1). Blood pressure was similar in wild‐type and Nox5 mice. Nox5 did not amplify angiotensin II effects. In R. prolixus, gastrointestinal smooth muscle contraction was blunted by Nox5 silencing, but not by VAS2870 (Nox1/2/4 inhibitor). Conclusions Nox5 is a pro‐contractile Nox isoform important in redox‐sensitive contraction. This involves calcium‐calmodulin and endoplasmic reticulum–regulated mechanisms. Our findings define a novel function for vascular Nox5, linking calcium and reactive oxygen species to the pro‐contractile molecular machinery in vascular smooth muscle cells

Estrutura populacional de espécies florestais não madeireiras em assentamento extrativista e de colonização, Porto Acre-AC.

Este trabalho elaborou um estudo sobre o comportamento ecológico de quatro espécies florestais importantes economicamente (Seringueira - Hevea brasiliensis Arg., Castanheira - Bertholletia excelsa H.B.K., Açaí - Euterpe precatoria Mart e Andiroba - Carapa guianensis Aublet), em dois tipos de assentamentos (PAE e PC recém criado)

NADPH oxidase 5 is a pro‐contractile Nox isoform and a point of cross‐talk for calcium and redox signaling‐implications in vascular function

Background: NADPH Oxidase 5 (Nox5) is a calcium‐sensitive superoxide‐generating Nox. It is present in lower forms and higher mammals, but not in rodents. Nox5 is expressed in vascular cells, but the functional significance remains elusive. Given that contraction is controlled by calcium and reactive oxygen species, both associated with Nox5, we questioned the role of Nox5 in pro‐contractile signaling and vascular function. Methods and Results: Transgenic mice expressing human Nox5 in a vascular smooth muscle cell–specific manner (Nox5 mice) and Rhodnius prolixus, an arthropod model that expresses Nox5 endogenoulsy, were studied. Reactive oxygen species generation was increased systemically and in the vasculature and heart in Nox5 mice. In Nox5‐expressing mice, agonist‐induced vasoconstriction was exaggerated and endothelium‐dependent vasorelaxation was impaired. Vascular structural and mechanical properties were not influenced by Nox5. Vascular contractile responses in Nox5 mice were normalized by N‐acetylcysteine and inhibitors of calcium channels, calmodulin, and endoplasmic reticulum ryanodine receptors, but not by GKT137831 (Nox1/4 inhibitor). At the cellular level, vascular changes in Nox5 mice were associated with increased vascular smooth muscle cell [Ca2+]i, increased reactive oxygen species and nitrotyrosine levels, and hyperphosphorylation of pro‐contractile signaling molecules MLC20 (myosin light chain 20) and MYPT1 (myosin phosphatase target subunit 1). Blood pressure was similar in wild‐type and Nox5 mice. Nox5 did not amplify angiotensin II effects. In R. prolixus, gastrointestinal smooth muscle contraction was blunted by Nox5 silencing, but not by VAS2870 (Nox1/2/4 inhibitor). Conclusions: Nox5 is a pro‐contractile Nox isoform important in redox‐sensitive contraction. This involves calcium‐calmodulin and endoplasmic reticulum–regulated mechanisms. Our findings define a novel function for vascular Nox5, linking calcium and reactive oxygen species to the pro‐contractile molecular machinery in vascular smooth muscle cells

Predictive biomarkers for response to EGFR-directed monoclonal antibodies for advanced squamous cell lung cancer

Writing assistance was funded by Eli Lilly and Company (no grant numbers apply).Peer reviewedPublisher PD