Detection of freeze injury in oranges using magnetic resonance imaging under motion conditions

Magnetic resonance imaging (MRI) is applied for on-line inspection of fruits. The aim of this work is to address the applicability of MRI for freeze injury detection in oranges directly on a distribution chain. Undamaged and damaged oranges are conveyed at 50 and 100 mm/s by a specially designed conveyor within a 4.7 T spectrometer obtaining fast low-angle shot images. An automatic segmentation algorithm is proposed that allows the discrimination between undamaged and damaged orange

Mutational processes molding the genomes of 21 breast cancers

All cancers carry somatic mutations. The patterns of mutation in cancer genomes reflect the DNA damage and repair processes to which cancer cells and their precursors have been exposed. To explore these mechanisms further, we generated catalogs of somatic mutation from 21 breast cancers and applied mathematical methods to extract mutational signatures of the underlying processes. Multiple distinct single- and double-nucleotide substitution signatures were discernible. Cancers with BRCA1 or BRCA2 mutations exhibited a characteristic combination of substitution mutation signatures and a distinctive profile of deletions. Complex relationships between somatic mutation prevalence and transcription were detected. A remarkable phenomenon of localized hypermutation, termed "kataegis," was observed. Regions of kataegis differed between cancers but usually colocalized with somatic rearrangements. Base substitutions in these regions were almost exclusively of cytosine at TpC dinucleotides. The mechanisms underlying most of these mutational signatures are unknown. However, a role for the APOBEC family of cytidine deaminases is proposed