Seismic interpretation and structural restoration of the Heligoland glaciotectonic thrust-fault complex: Implications for multiple deformation during (pre-)Elsterian to Warthian ice advances into the southern North Sea Basin

Despite a long history of research, the locations of former ice-margins in the North Sea Basin are still uncertain. In this study, we present new palaeogeographic reconstructions of (pre-) Elsterian and Warthian ice-margins in the southeastern North Sea Basin, which were previously unknown. The reconstructions are based on the integration of palaeo-ice flow data derived from glaciotectonic thrusts, tunnel valleys and mega-scale glacial lineations. We focus on a huge glaciotectonic thrust complex located about 10 km north of Heligoland and 50 km west of the North Frisian coast of Schleswig-Holstein (Northern Germany). Multi-channel high-resolution 2D seismic reflection data show a thrust-fault complex in the upper 300 ms TWT (ca. 240 m) of seismic data. This thrust-fault complex consists of mainly Neogene delta sediments, covers an area of 350 km2, and forms part of a large belt of glaciotectonic complexes that stretches from offshore Denmark via northern Germany to Poland. The deformation front of the Heligoland glaciotectonic complex trends approximately NNE-SSW. The total length of the glaciotectonic thrust complex is approximately 15 km. The thrust faults share a common detachment surface, located at a depth of 250–300 ms (TWT) (200–240 m) below sea level. The detachment surface most probably formed at a pronounced rheological boundary between Upper Miocene fine-grained pro-delta deposits and coarser-grained delta-front deposits, although we cannot rule out that deep permafrost in the glacier foreland played a role for the location of this detachment surface. Restored cross-sections reveal the shortening of the complex along the detachment to have been on average 23% (ranging from ca. 16%–50%). The determined ice movement direction from east-southeast to southeast suggests deformation by an ice advance from the Baltic region. The chronospatial relationship of the thrust-fault complex and adjacent northwest-southeast to northeast-southwest trending Elsterian tunnel valleys implies a pre-Elsterian (MIS 16?) age of the glaciotectonic complex. However, the age of these Elsterian tunnel valleys is poorly constrained and the glaciotectonic complex of Heligoland may also have been formed during an early Elsterian ice advance into the southeastern North Sea Basin. The glaciotectonic complex underwent further shortening and the Elsterian tunnel-valley fills that were incised into the glaciotectonic complex were partly deformed during the Saalian Drenthe and Warthe (1) ice advances

Pervasive deformation of an oceanic plate and relationship to large >Mw 8 intraplate earthquakes: The northern Wharton Basin, Indian Ocean

Large-magnitude intraplate earthquakes within the ocean basins are not well understood. The Mw 8.6 and Mw 8.2 strike-slip intraplate earthquakes on 11 April 2012, while clearly occurring in the equatorial Indian Ocean diffuse plate boundary zone, are a case in point, with disagreement on the nature of the focal mechanisms and the faults that ruptured. We use bathymetric and seismic reflection data from the rupture area of the earthquakes in the northern Wharton Basin to demonstrate pervasive brittle deformation between the Ninetyeast Ridge and the Sunda subduction zone. In addition to evidence of recent strike-slip deformation along approximately north-south–trending fossil fracture zones, we identify a new type of deformation structure in the Indian Ocean: conjugate Riedel shears limited to the sediment section and oriented oblique to the north-south fracture zones. The Riedel shears developed in the Miocene, at a similar time to the onset of diffuse deformation in the central Indian Ocean. However, left-lateral strike-slip reactivation of existing fracture zones started earlier, in the Paleocene to early Eocene, and compartmentalizes the Wharton Basin. Modeled rupture during the 11 April 2012 intraplate earthquakes is consistent with the location of two reactivated, closely spaced, approximately north-south–trending fracture zones. However, we find no evidence for WNW-ESE–trending faults in the shallow crust, which is at variance with most of the earthquake fault models

Relevance of field observations as boundary conditions for understanding ice-sheet-ocean interactions

The direct contact of warm ocean water with the front and base of ice shelves is the main driver for accelerated mass loss of the Antarctic ice sheet. We present a compilation of observations from various projects and methodological approaches applied over the last decade along the Dronning Maud Land coast and highlight their importance for understanding the ice-ocean interactions. With a focus on the Ekström ice shelf, these include spatially continuous seismic observations in combination with airborne gravity inversion to yield sub-shelf bathymetry and geomorphological evidence of past ice-flow activity; ice-dynamic numerical modelling to investigate the role of seafloor/subglacial substrate characteristics to enhance or reduce ice-sheet extent and advance/retreat rates; sub-shelf CTD measurements to determine ocean properties driving basal melting; satellitebased remote sensing to determine ice-shelf height changes and spatially-distributed basal melting; and point measurements of basal melt with surface-based phase-sensitive radar to determine ocean-driven melt and validate remote-sensing products. As the Dronning Maud Land coast plays a critical role in preconditioning the water mass of the coastal current before it enters the Filcher ice-shelf cavity, we argue that a coordinated inter- and transdisciplinary observational network is required to facilitate monitoring a potential ice-sheet mass loss in this part of Antarctica

What lies beneath: A detailed bathymetry of the sea-floor below Ekström Ice Shelf, East Antarctica

An extensive grid of seismic reflection data collected on Ekstro¨m Ice Shelf, East Antarctica, between 2010–2018, using an on-ice vibroseis source and snowstreamer, are used to make a detailed bathymetry map of the sea floor and ice-shelf cavity. The maps shows a deep sea-floor trough, likely a paleao-ice stream, under the western side of the ice shelf. The trough contains a number of points of higher topography, indicating probable former grounding line positions. At the shelf front a sill running across the width of the shelf has implications for ocean circulation and thus ice-ocean interaction and ice shelf melt. This new bathymetry is markedly different from previous models, which show a generally flat and shallow sea floor in the region. This is presumably the case for many of the smaller ice-shelves in Dronning Maud Land, which highlights the need for better bathymetry measurements in these key threshold region

Non-Invasive In Vivo Imaging of Tumor-Associated CD133/Prominin

detection of cancer stem cells is of great importance. detection of CD133/prominin, a cancer stem cell surface marker for a variety of tumor entities. The CD133-specific monoclonal antibody AC133.1 was used for quantitative fluorescence-based optical imaging of mouse xenograft models based on isogenic pairs of CD133 positive and negative cell lines. A first set consisted of wild-type U251 glioblastoma cells, which do not express CD133, and lentivirally transduced CD133-overexpressing U251 cells. A second set made use of HCT116 colon carcinoma cells, which uniformly express CD133 at levels comparable to primary glioblastoma stem cells, and a CD133-negative HCT116 derivative. Not surprisingly, visualization and quantification of CD133 in overexpressing U251 xenografts was successful; more importantly, however, significant differences were also found in matched HCT116 xenograft pairs, despite the lower CD133 expression levels. The binding of i.v.-injected AC133.1 antibodies to CD133 positive, but not negative, tumor cells isolated from xenografts was confirmed by flow cytometry. imaging of tumor-associated CD133 is feasible and that CD133 antibody-based tumor targeting is efficient. This should facilitate developing clinically applicable cancer stem cell imaging methods and CD133 antibody-based therapeutics

Gene Expression Profiling in Cells with Enhanced γ-Secretase Activity

BACKGROUND: Processing by gamma-secretase of many type-I membrane protein substrates triggers signaling cascades by releasing intracellular domains (ICDs) that, following nuclear translocation, modulate the transcription of different genes regulating a diverse array of cellular and biological processes. Because the list of gamma-secretase substrates is growing quickly and this enzyme is a cancer and Alzheimer's disease therapeutic target, the mapping of gamma-secretase activity susceptible gene transcription is important for sharpening our view of specific affected genes, molecular functions and biological pathways. METHODOLOGY/PRINCIPAL FINDINGS: To identify genes and molecular functions transcriptionally affected by gamma-secretase activity, the cellular transcriptomes of Chinese hamster ovary (CHO) cells with enhanced and inhibited gamma-secretase activity were analyzed and compared by cDNA microarray. The functional clustering by FatiGO of the 1,981 identified genes revealed over- and under-represented groups with multiple activities and functions. Single genes with the most pronounced transcriptional susceptibility to gamma-secretase activity were evaluated by real-time PCR. Among the 21 validated genes, the strikingly decreased transcription of PTPRG and AMN1 and increased transcription of UPP1 potentially support data on cell cycle disturbances relevant to cancer, stem cell and neurodegenerative diseases' research. The mapping of interactions of proteins encoded by the validated genes exclusively relied on evidence-based data and revealed broad effects on Wnt pathway members, including WNT3A and DVL3. Intriguingly, the transcription of TERA, a gene of unknown function, is affected by gamma-secretase activity and was significantly altered in the analyzed human Alzheimer's disease brain cortices. CONCLUSIONS/SIGNIFICANCE: Investigating the effects of gamma-secretase activity on gene transcription has revealed several affected clusters of molecular functions and, more specifically, 21 genes that hold significant potential for a better understanding of the biology of gamma-secretase and its roles in cancer and Alzheimer's disease pathology

Specific features of the upper sedimentary cover and slump structures in the NW Pacific and the Bering Sea based on seismoacoustic profiling data

The authors studied geology-geophysical data that had been obtained during German RV «Sonne» Cruise in NW Pacific organized in frames of the Russian-German Project KALMAR («Kurile-Kamchatka and Aleutian Marginal sea-island arc systems: geodynamic and climate interaction in space and time»). The profiling was carried out using PARASOUND P70 system, including narrow-beam system and sub-bottom profiler. The study showed that besides undisturbed sedimentation, landside processes are widely spread in open areas of the ocean and the Bering Sea as well as deposits caused by strong submarine currents. Landslides were revealed on the slopes of the Emperor Ridge in the NW Pacific and the Shirshov Ridge submarine mountains, in canyons near Eastern Kamchatka coasts and apparently in many other areas