832 research outputs found

    Photoperiod-sensitivity genes (Ppd-1) : quantifying their effect on the photoperiod response model in wheat

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    Coupling anthesis date to the most suitable environmental conditions is critical for wheat (Triticum aestivum L.) adaptation and yield potential. Development to anthesis is controlled by temperature and photoperiod. Response to photoperiod is chiefly modulated by Ppd-1 genes, but their effect on the quantitative response to photoperiod of (i) time to anthesis and (ii) pre-anthesis phases remains largely unknown. A photoperiod-sensitive spring cultivar, Paragon, and near-isogenic lines of it carrying different combinations of Ppd-1a insensitivity alleles were tested under a wide range of photoperiods, including switches in photoperiod at the onset of stem elongation. Using multimodel inference we found that Ppd-1a alleles reduced photoperiod sensitivity of (i) emergence to anthesis and (ii) emergence to onset of stem elongation, both in a less than additive manner, while threshold photoperiod and intrinsic earliness were unaffected. Sensitivity to current photoperiod from onset of stem elongation to flag leaf and from then to anthesis was milder than for previous phases and was not related to variability in Ppd-1. However, ‘memory’ effects of previously experienced photoperiod on the duration from onset of stem elongation to flag leaf were related to variability in Ppd-1. The characterization and quantification provided here of the effects on development of Ppd-1 allelic combinations should help increase accuracy of genotype-to-phenotype models in predicting wheat phenology.EEA PergaminoFil: Perez Gianmarco, Thomas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Ecofisiología; Argentina. CONICET-UNNOBA.CITNOBA; ArgentinaFil: Severini, Alan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Ecofisiología; ArgentinaFil: González, Fernanda G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Sección Ecofisiología; Argentina. CONICET-UNNOBA.CITNOBA; Argentin

    mejora continua del proceso de electrospining por medio del análisis de procesos: caso de estudio CU UAEM Valle de México

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    Se tiene como misión el innovar e implementar nuevas tecnologías con conocimientos adquiridos de nuestra formación, aplicándolo en el desarrollo de la máquina de electrospínning. Iniciamos por la producción de biotecnología con la preparación y proceso de la solución de Difluoruro de Polivinilideno (PVDF) con el diluyente Dimetilformamida (DMF). Este proceso se emplea a alto voltaje realizando un campo electrostático, este atraerá partículas de la solución o fluido polimérico formando un cono inyector hacia una superficie recolectora la cual formara y solidificara partículas micro y nanofibras, las cuales formaran membranas.Its mission is to improve, create, innovate and implement new technologies with the knowledge acquired from our training, applying it in the development of the electrospinning machine. In this case we started with the production of biotechnology with respect to the preparation and processing of the solution of Polyvinylidene Difluoride (PVDF) with a thinner material such as Dimethylformamide (DMF), as well as the construction of a prototype electrospinning machine.This process employs high voltage for an electrostatic field, which will attract particles from the solution or polymeric fluid thus forming an injector cone towards a collecting surface which will form and solidify micro and nanofibres particles, which in turn will form membrane

    Emerging Biomarkers and Clinical Implications in Endometrial Carcinoma

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    Endometrial cancer (EmCa) is the most common type of gynecological cancer. EmCa is the fourth most common cancer in the United States, which has been linked to increased incidence of obesity. EmCa can be classified into two main types: Type I and Type II, which include the major histological subtypes. Type I EmCa is hormonally driven, less aggressive, and has a more favorable prognosis. In contrast, Type II EmCa grows independently of hormonal signals, is more aggressive, and generally has an unfavorable prognosis. Various tumor biomarkers [i.e., tumor suppressor p53, hypoxia-inducible factor 1-alpha (HIF1-α), human epidermal growth factor receptor 2 (HER2/neu), and vascular endothelial growth factor (VEGF)] have been identified in EmCa. Biomarkers of treatment effectiveness involve immunosuppressive factors targeted by microRNA (miRNA)-based therapy. However, there are no reliable biomarker tests for early detection of EmCa and treatment effectiveness. A potential new biomarker is Notch, Interleukin-1, leptin crosstalk outcome (NILCO) that could affect the progression of Type II EmCa. NILCO expression in EmCa might be dependent on patient’s obesity status. This chapter presents updated information on these, and other potential emerging biomarkers for EmCa, and discusses current challenges and clinical implications on this area of research

    Cómo afectan los genes de sensibilidad al fotoperíodo PPD-1 al desarrollo del trigo a antesis?

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    La sensibilidad al fotoperiodo afecta la adaptación del trigo al ambienteal modifiar: (i) la fecha de antesis, (ii) la duración de las etapas vegetativa y reproductiva previas a antesis, y (iii) la generación de estructuras durante dichas etapas. Utilizando líneas isogénicas para genes de sensibilidad al fotoperiodo (Ppd-1) se observó que el orden de insensibilidad para todas las etapas estudiadas fue PpdD1a>Ppd-A1a>Ppd-B1a. No se detectó especificidad de ningún alelo con alguna etapa particular, ni tampoco efectos aditivos entre los alelos.EEA PergaminoFil: Slafer, Gustavo. Universidad de Lleida. Instituto Catalana de Investigación y Estudios Avanzados (ICREA). Departamento Producción Vegetal y Ciencias Forestales y AGROTECNIO; España.Fil: González, Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Ecofisiología; Argentina. Centro de Investigacion y Transferencia del Noroeste de la Provincia de Buenos Aires (CIT NOBA); Argentina

    Survivability of entrapped Lactobacillus rhamnosus in liquid- and gel-core alginate beads during storge and simulated gastrointestinal conditions

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    L. rhamnosus cells were encapsulated in liquid-core (LCBR) and gel-core (GCBR) calcium alginate beads, and cell survivability under storage conditions and simulated gastrointestinal conditions were evaluated, and compared with that of non-encapsulated cells. The average external diameters of both beads (1.37 - 0.25 mm) were non-significantly dierent, and the average thickness of alginate gelled layer in LCBR was of 0.27 - 0.01 mm. The bacteria entrapped into LCBR tended to gather together forming clusters in the bulk of the liquid phase of the bead, whereas the bacteria entrapped into GCBR were compartmentalized in the gelled bead biopolymer matrix. LCBR showed significant lower hardness and chewiness, higher cohesiveness, and comparable springiness values than GCBR. Cells survivability under storage and simulated gastrointestinal conditions was significantly higher in LCBR than in GCBR and for the non-encapsulated free cells

    Estudio de la respuesta inmune frente a la vacunación con virus respiratorio sincitial bovino (VRSB) inactivado en bovinos. Evaluación de la inmunidad pasiva

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    El objetivo de este trabajo fue evaluar el título y la duración de anticuerpos neutralizantes (AN) en vacas inmunizadas con una vacuna experimental inactivada para el virus respiratorio sincitial bovino (VRSB) y los niveles y la duración de los anticuerpos maternales anti-VRSB transferidos a través del calostrado en los terneros nacidos. Se inoculó un grupo de seis vacas preñadas con una vacuna inactivada de VRSB 90 y 60 días antes del parto. El grupo control estaba formado por seis vacas sin vacunar. Se obtuvieron muestras de suero de las vacas a los días 90, 60, 30 antes del parto y 0, 60 y 120 días posteriores al parto. Con respecto a los terneros, se recolectaron muestras de suero de ambos grupos a las 48 horas posparto y 30, 60, 90 y 120 días luego del nacimiento. La detección de anticuerpos específicos contra el VRSB se realizó mediante seroneutralización viral. En los terneros se determinaron proteínas totales e inmunoglobulina G total a las 48 horas posparto. Solo las vacas vacunadas seroconvirtieron a los 60 días después del refuerzo y los títulos de anticuerpos permanecieron elevados 180 días después de este. Los terneros recién nacidos mostraron una transferencia pasiva efectiva de anticuerpos maternos específicos para el VRSB. En este trabajo fue posible corroborar la inducción y duración de los anticuerpos específicos contra el VRSB en vacas vacunadas con una vacuna inactivada así como en sus respectivos terneros.The aim of this work was to evaluate the titer and duration of neutralizing antibodies in cows immunized with an inactivated experimental vaccine for BRSV and the levels and duration of anti-BRSV maternal antibodies in calves born. A group of six pregnant cows was inoculated with a inactivated BRSV vaccine, at 90 and 60 days before calving. As a control group, six animals were mock inoculated. Cows´ serum samples were obtained at days 90, 60, 30 before calving and at 0, 60 and 120 days postpartum. Sera from calves were obtained at 48-72 hours postpartum, 30, 60, 90 and 120 days after birth. The kinetic of serum specific antibodies from inoculated animals and newborn calves was analyzed by a serum neutralization assay. Only the vaccinated cows serocoverted 60 days post booster and antibody titers remained high 180 days post booster. Six newborn calves showed an effective passive transfer of specific BRSV maternal antibodies. In this work, it was possible to determine antibody levels against BRSV and their duration after vaccination of cows and calves born using an inactivated vaccine.EEA Marcos JuárezFil: Margineda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; ArgentinaFil: Ferella, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pérez Aguirreburualde, María Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Sammarruco, Romina Ayelén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Toledo, Gabriela Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
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