36 research outputs found
Lack of long-term acclimation in Antarctic encrusting species suggests vulnerability to warming
Marine encrusting communities play vital roles in benthic ecosystems and have major economic implications with regards to biofouling. However, their ability to persist under projected warming scenarios remains poorly understood and is difficult to study under realistic conditions. Here, using heated settlement panel technologies, we show that after 18 months Antarctic encrusting communities do not acclimate to either +1 °C or +2 °C above ambient temperatures. There is significant up-regulation of the cellular stress response in warmed animals, their upper lethal temperatures decline with increasing ambient temperature and population genetic analyses show little evidence of differential survival of genotypes with treatment. By contrast, biofilm bacterial communities show no significant differences in community structure with temperature. Thus, metazoan and bacterial responses differ dramatically, suggesting that ecosystem responses to future climate change are likely to be far more complex than previously anticipated
Leukocytes and systemic inflammatory response syndrome as prognostic factors in pulmonary embolism patients
Guidance for the use of thrombolytic therapy for the treatment of venous thromboembolism
Post-dispersal seed removal by ground-feeding rodents in tropical peatlands, Central Kalimantan, Indonesia
Uptake of hepatitis B-HIV co-infection screening and management in a resource limited setting
Characterization and mapping of hematite ore mineral classes using hyperspectral remote sensing technique: a case study from Bailadila iron ore mining region
MS/MS analysis and imaging of lipids across Drosophila brain using secondary ion mass spectrometry
Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry
Lipidomics studies the large-scale changes in nonwater-soluble metabolites (lipids) accompanying perturbations of biological systems. Because lipids are involved in crucial biological mechanisms, there is a growing scientific interest in using lipidomic approaches to understand the regulation of the lipid metabolism in all eukaryotic and prokaryotic organisms. Lipidomics is a powerful tool in system biology that can be used together with genomics, transcriptomics, and proteomics to answer biological questions arising from various scientific areas such as environmental sciences, pharmacology, nutrition, biophysics, cell biology, physiology, pathology, and disease diagnostics. One of the main challenges for lipidomic analysis is the range of concentrations and chemical complexity of different lipid species. In this chapter, we present a lipidomic approach that combines sample preparation, chromatographic, and intrasource ionization separation coupled to mass spectrometry for analyzing a broad-range of lipid molecules in biological samples