Those Days in Muramatsu: Diary by Mrs. Yumi Goto

Introduction by Grant K. Goodman, and "Days of Ambivalence" by Elizabeth Schultz."Like other personal writings, Yumi Goto's memoir, _Those Days in Muramatsu_, is both private and public. It reflects upon an interlude not only in her personal history but also in the social history of Japan. More precisely, Mrs. Goto's memoir reflects upon those days from September to December 1945 when she served as an interpreter for the railroad company working with the American military stationed as part of the Allied Occupation in Muramatsu, a small rural town in the province of Niigata in northern Japan; as such, the memoir illuminates a significant moment in the history of Japan-American relations, during which the lives of diverse people in Muramatsu were connected with the lives of 1500 American GIs." --- Introduction by Grant K. GoodmanUniversity of Kansas, Center for East Asian Studie

保育内容総論の研究（１） －宍戸健夫の保育カリキュラム研究分析－

The focus of this study is on “General Theory in Childcare and Education,” a subject introduced, with a crucial role to play, in the curricula for the education and training of nursery school and kindergarten teachers. The key objective of this study is to reconsider the theoretical foundations of this subject in approaching its progressive implementation.Other subjects related to the content of childcare and education within the curricula for the education and training of nursery school and kindergarten teachers include, “Curriculum Theory in Kindergarten Education,” “Early Childhood Education Methods,” “Principles of Childcare and Education,” and “Principles of Early Childhood Education.” Therein, the subject most closely related to General Theory in Childcare and Education is “Curriculum Theory in Early Childhood Education.” However, to date, there has been little investigation into the content of General Theory in Childcare and Education at the syllabus level and few clear investigations into its ranking in relation to other subjects. Therefore, in this study, we engage in a structured theoretical inquiry into “General Theory in Childcare and Education” that can assist with general teaching in the field of childhood care and education. This paper reviews prior research into early childhood education curriculum studies in Japan and aims to elicit suggestions that will contribute to the conceptualization of General Theory in Childcare and Educatio

Relaxation of the Plant Cell Wall Barrier via Zwitterionic Liquid Pretreatment for Micelle‐Complex‐Mediated DNA Delivery to Specific Plant Organelles

Targeted delivery of genes to specific plant organelles is a key challenge for fundamental plant science, plant bioengineering, and agronomic applications. Nanoscale carriers have attracted interest as a promising tool for organelle-targeted DNA delivery in plants. However, nanocarrier-mediated DNA delivery in plants is severely hampered by the barrier of the plant cell wall, resulting in insufficient delivery efficiency. Herein, we propose a unique strategy that synergistically combines a cell wall-loosening zwitterionic liquid (ZIL) with a peptide-displaying micelle complex for organelle-specific DNA delivery in plants. We demonstrated that ZIL pretreatment can enhance cell wall permeability without cytotoxicity, allowing micelle complexes to translocate across the cell wall and carry DNA cargo into specific plant organelles, such as nuclei and chloroplasts, with significantly augmented efficiency. Our work offers a novel concept to overcome the plant cell wall barrier for nanocarrier-mediated cargo delivery to specific organelles in living plants

Spontaneous Production of Interleukin-5 and Its Heterogeneous Effect on Eosinophils in an Adult T-Cell Leukemia Patient

ABSTRACTWe examined the functional heterogeneity of eosinophils from an adult T-cell leukemia (ATL) patient with eosinophilia. A 63-year-old man was admitted to our hospital because of lymphadenopathy. The leukocyte count was 10 400 /mm3, with 36.0% eosinophils and 3.0% abnormal lymphocytes. The diagnosis of ATL was based on the presence in serum of anti- human T-cell lymphotrophic virus-1 antibody and on histologic demonstration of ATL cells. The mononuclear cells spontaneously produced eosinophil-related cytokines (granulocyte-macrophage colony stimulating factor, 5600pg/mL; interleukin (IL)-5, 375pg/mL). Peripheral eosinophils were fractionated into normodense eosinophils (NE) and hypodense eosinophils (HE) by a Percoll density gradient method, and these cells were compared in terms of several heterogeneous functions. The NE were more chemotactically attracted to IL-5 than the HE. More apoptotic cells appeared among the NE than among the HE and this difference was correlated with the positive rate of Fas antigen on eosinophils. Survival of the HE was longer than that of the NE. Survival of the HE was prolonged by IL-5 stimulation, but survival of the NE was not. These data suggest that functionally heterogeneous eosinophils were present in this ATL patient with eosinophilia and that IL-5 enhanced this heterogeneity. The response of eosinophils to IL-5 may have contributed to the patho- genesis of eosinophilia in this patient

The Fundamental Planes of E+A galaxies and GALEX UV-excess early-type galaxies: Revealing their intimate connection

Strong Balmer absorption lines and the lack of Ha and [OII] emission lines signify that E+As are post-starburst systems. Recent studies suggest that E+As may undergo the transition from the `blue cloud' to the `red sequence' and eventually migrate to red sequence ETGs. An observational validation of this scenario is to identify the intervening galaxy population between E+As and the red-sequence. Motivated by recent findings with GALEX that a large fraction of ETGs exhibit UV-excess as a sign of RSF, we investigate the possible connection of the UV-excess galaxies to E+As. In particular, we examine the FP scaling relations of the largest sample of ~1,000 E+As selected from the SDSS and ~20,000 morphologically-selected SDSS ETGs with GALEX UV data. The FP parameters, combined with stellar population indicators, reveal a certain group of UV-excess ETGs that bridges between E+As and quiescent red galaxies. The newly identified galaxies are the post-starburst systems characterized by UV-excess but no Ha emission. This is a conceptual generalisation of "E+A", in that the Balmer absorption line in the "E+A" definition is replaced with UV-optical colours that are far more sensitive to RSF than the Balmer lines. We refer to these UV-excess galaxies as "E+a" galaxies, which stands for elliptical ("E") galaxies with a minority of A-type ("a") young stars. The species are either (1) galaxies that experienced starbursts weaker than those observed in E+As (1~10% of E+As, "mild E+As") or (2) the products of passively evolved E+As after quenching star formation quite a while ago (~1 Gyr, "old E+As"). We suggest that the latter type of E+a galaxies represents the most recent arrival to the red sequence in the final phase of the "E+A" to "red early-type" transition. (Abridged)Comment: 15 pages, 15 figures, Accepted for publication in MNRA

足関節の視覚性運動錯覚中に活動する脳領域

Abstract. [Purpose] This study sought to localize the brain activity prompted by visual motor illusion (VMI) of the ankle movement. [Participants and Methods] We randomly applied VMI condition to the left and right lower limbs of 13 healthy subjects. The VMI condition required watching a video to induce VMI; the recording featured an ankle dorsiflexion movement on the non-measuring side in the first-person perspective. The left and right VMI conditions were measured three sets using a rest-task-rest block design using functional near-infrared spectroscopy. Oxygenated hemoglobin (oxy - Hb) during rest and task was measured under two conditions. Oxy - Hb of the two conditions were calculated average values of three sets and compared left and right VMI condition. During the VMI condition, a visual analog scale (VAS) was evaluated the degree of kinesthetic illusion. VAS of both VMI condition was compared using t test. [Results] VAS of the left VMI condition was higher when compared with the right VMI condition. In both conditions, oxy - Hb in the premotor area was significantly increased during the VMI condition. [Conclusion] VMI of the ankle movement induces increased oxy - Hb in the premotor area of healthy subjects.東京都立大学学位論文甲第1066号副論

A Synthetic Multidomain Peptide That Drives a Macropinocytosis-Like Mechanism for Cytosolic Transport of Exogenous Proteins into Plants

Direct delivery of proteins into plants represents a promising alternative to conventional gene delivery for probing and modulating cellular functions without the risk of random integration of transgenes into the host genome. This remains challenging, however, because of the lack of a protein delivery tool applicable to diverse plant species and the limited information about the entry mechanisms of exogenous proteins in plant cells. Here, we present the synthetic multidomain peptide (named dTat-Sar-EED4) for cytosolic protein delivery in various plant species via simple peptide-protein coincubation. dTat-Sar-EED4 enabled the cytosolic delivery of an active enzyme with up to ∼20-fold greater efficiency than previously described cell-penetrating peptides in several model plant systems. Our analyses using pharmacological inhibitors and transmission electron microscopy revealed that dTat-Sar-EED4 triggered a unique endocytic mechanism for cargo protein internalization. This endocytic mechanism shares several features with macropinocytosis, including the dependency of actin polymerization, sensitivity to phosphatidylinositol-3 kinase activity, and formation of membrane protrusions and large intracellular vesicles (>200 nm in diameter), even though macropinocytosis has not been identified to date in plants. Our study thus presents a robust molecular tool that can induce a unique cellular uptake mechanism for the efficient transport of bioactive proteins into plants