WHI-2 Regulates Intercellular Communication via a MAP Kinase Signaling Complex

he formation of the fungal mycelial network is facilitated by somatic cell fusion of germinating asexual spores (or germlings). Neurospora crassa germlings in close proximity display chemotropic growth that is dependent upon an intracellular network of mitogen-activated protein kinase (MAPK) signaling cascades. Approximately 80 genes involved in intercellular communication and fusion have been identified, including three mutants with similar morphological phenotypes: Δwhi-2, Δcsp-6, and Δamph-1. Here we show that WHI-2 localizes to the cell periphery and regulates endocytosis, mitochondrial organization, sporulation, and cell fusion. WHI-2 was required to transduce signals through a conserved MAPK pathway (NRC-1/MEK-2/MAK-2) and target transcription factors (PP-1/ADV-1). The amph-1 locus encodes a Bin/Amphiphysin/Rvs domain-containing protein and mis-expression of whi-2 compensated for the cell fusion and endocytosis deficiencies of a Δamph-1 mutant. The csp-6 locus encodes a haloacid dehalogenase phosphatase whose activity was essential for cell fusion. Although fusion-deficient with themselves, cells that lacked whi-2, csp-6, or amph-1 showed a low frequency of chemotropic interactions with wild type cells. We hypothesize that WHI-2 could be important for signal perception during chemotropic interactions via a role in endocytosis

Improving cost-efficiency for MPs density separation by zinc chloride reuse

The methodology used to extract and quantify microplastics (MPs) in aquatic systems are still not standardized. Salt saturated solutions, such as sodium chloride (NaCl), zinc chloride (ZnCl2) and/or sodium iodide (NaI), are normally added to separate dense plastics from aquatic samples. However, the most effective reagents are also the most expensive (e.g. ZnCl2 and NaI). To decrease this cost, a reuse process of the salt solutions should be applied. The reuse process has been widely investigated for the NaI solution neglecting the ZnCl2. Hence, the aim of this study was to present a simple methodology to reuse the ZnCl2 solution ensuring the efficiency of the product. Results of the present study showed that ZnCl2 solution could be reused at least five times maintaining an efficiency above 95 %. •The ZnCl2 reuse decreases the cost of the methodology.•The efficiency of ZnCl2 solution after five filtrations remains above 95 % (all polymers are detected and recovered).•The use of this salt solution is the most cost-effective methodology to isolate MPs from aquatic samples.publishe

Spin torque ferromagnetic resonance with magnetic field modulation

We demonstrate a technique of broadband spin torque ferromagnetic resonance (ST-FMR) with magnetic field modulation for measurements of spin wave properties in magnetic nanostructures. This technique gives great improvement in sensitivity over the conventional ST-FMR measurements, and application of this technique to nanoscale magnetic tunnel junctions (MTJs) reveals a rich spectrum of standing spin wave eigenmodes. Comparison of the ST-FMR measurements with micromagnetic simulations of the spin wave spectrum allows us to explain the character of low-frequency magnetic excitations in nanoscale MTJs.Comment: Also see: http://faculty.sites.uci.edu/krivorotovgroup

Phase diagram of a random-anisotropy mixed-spin Ising model

We investigate the phase diagram of a mixed spin-1/2--spin-1 Ising system in the presence of quenched disordered anisotropy. We carry out a mean-field and a standard self-consistent Bethe--Peierls calculation. Depending on the amount of disorder, there appear novel transition lines and multicritical points. Also, we report some connections with a percolation problem and an exact result in one dimension.Comment: 8 pages, 4 figures, accepted for publication in Physical Review

Nuclear shadowing from exclusive quarkonium photoproduction at the BNL RHIC and CERN LHC

The photonuclear production of vector mesons in ultraperipheral heavy ion collisions is investigated within the collinear approach using different parameterizations for the nuclear gluon distribution. The integrated cross section and the rapidity distribution for the $AA \to V AA$ ($V = J/\Psi, \Upsilon$) process are computed for energies of RHIC and LHC. A comparison with the recent PHENIX data on coherent production of $J/\Psi$ mesons is also presented. We demonstrate that the study of the exclusive quarkonium photoproduction can be used to constrain the nuclear effects in the gluon distribution.Comment: 8 pages, 4 figures, 2 tables. Version to be published in Physical Review

Hydrodynamic limit for weakly asymmetric simple exclusion processes in crystal lattices

We investigate the hydrodynamic limit for weakly asymmetric simple exclusion processes in crystal lattices. We construct a suitable scaling limit by using a discrete harmonic map. As we shall observe, the quasi-linear parabolic equation in the limit is defined on a flat torus and depends on both the local structure of the crystal lattice and the discrete harmonic map. We formulate the local ergodic theorem on the crystal lattice by introducing the notion of local function bundle, which is a family of local functions on the configuration space. The ideas and methods are taken from the discrete geometric analysis to these problems. Results we obtain are extensions of ones by Kipnis, Olla and Varadhan to crystal lattices.Comment: 41 pages, 7 figure

Limpeza de campo na Serra do Sudeste, RS: efeito do corte e queima de plantas lenhosas, visando aumentar as áreas de pastoreio.

Sistema da produção da região; O problema e a pesquisa realizada; Resultados; Conclusões.bitstream/item/110551/1/LIMPEZA-DE-CAMPO-NA-SERRA.pd

Envelhecimento, trabalho e tempo livre: elaborando projetos de vida.

bitstream/item/71542/1/ID-30980.pd