Predicting the abundance of African horse sickness vectors in South Africa using GIS and artificial neural networks

African horse sickness (AHS) is a disease that is endemic to sub-Saharan Africa and is caused by a virus potentially transmitted by a number of Culicoides species (Diptera: Ceratopogonidae) including Culicoides imicola and Culicoides bolitinos. The strong association between outbreaks of AHS and the occurrence in abundance of these two Culicoides species has enabled researchers to develop models to predict potential outbreaks. A weakness of current models is their inability to determine the relationships that occur amongst the large number of variables potentially influencing the population density of the Culicoides species. It is this limitation that prompted the development of a predictive model with the capacity to make such determinations. The model proposed here combines a geographic information system (GIS) with an artificial neural network (ANN). The overall accuracy of the ANN model is 83%, which is similar to other stand-alone GIS models. Our predictive model is made accessible to a wide range of practitioners by the accompanying C. imicola and C. bolitinos distribution maps, which facilitate the visualisation of the model’s predictions. The model also demonstrates how ANN can assist GIS in decision-making, especially where the data sets incorporate uncertainty or if the relationships between the variables are not yet known

Expression of mucosal chemokines TECK/CCL25 and MEC/CCL28 during fetal development of the ovine mucosal immune system

CCL25/TECK and CCL28/MEC are CC chemokines primarily expressed in thymic dendritic cells and mucosal epithelial cells. The cognate receptors of CCL25 and CCL28, CCR9 and CCR10, respectively, are mainly expressed on T and B lymphocytes. In human, mouse and pig, CCL25 and CCL28 play a key role in the segregation and the compartmentalization of the mucosal immune system through recruitment of immune cells to specific locations. However, little is known about their role in the ontogeny of the mucosal immune system during fetal development. In the present paper, we report the cloning and the sequencing of ovine CCL25, CCL28, CCR9 and CCR10 and the subsequent assessment of their mRNA expression by q-polymerase chain reaction in several tissues, including thymus, gut-associated lymphoid tissue and mammary gland, from young and adult sheep and in the fetal lamb during the development of the immune system. CCL25 mRNA was highly expressed in thymus and gut while CCL28 mRNA was more expressed in large intestine, trachea, tonsils and mammary gland, especially at the end of gestation. These results are consistent with observations in other species suggesting similar roles for these chemokines in sheep. In fetuses, mRNA of CCL25, CCL28 and their receptors are expressed early in the thymus and mucosal tissues, including the small intestine and the nasal mucosa. Furthermore, their expression increased towards the end of gestation. Consequently, we hypothesize that CCL25 and CCL28 play an important role in the lymphocyte colonization of fetal tissues, enabling the development of a functional immune system

Liver sinusoidal endothelial cells - gatekeepers of hepatic immunity.

Liver sinusoidal endothelial cells (LSECs) line the low shear, sinusoidal capillary channels of the liver and are the most abundant non-parenchymal hepatic cell population. LSECs do not simply form a barrier within the hepatic sinusoids but have vital physiological and immunological functions, including filtration, endocytosis, antigen presentation and leukocyte recruitment. Reflecting these multifunctional properties, LSECs display unique structural and phenotypic features that differentiate them from the capillary endothelium present within other organs. It is now clear that LSECs have a critical role in maintaining immune homeostasis within the liver and in mediating the immune response during acute and chronic liver injury. In this Review, we outline how LSECs influence the immune microenvironment within the liver and discuss their contribution to immune-mediated liver diseases and the complications of fibrosis and carcinogenesis

Postharvest biology and technology of plum

Games can provide an effective and replicable space in which stakeholders learn skills necessary for deliberative and pluralist policymaking. These skills are especially important for “nexus” policy issues that are typically characterised by multiple, competing problem frames involving overlapping networks of stakeholders. In this position paper, we describe three serious games that serve as a space for players (stakeholders) and researchers to jointly explore alternative solutions to complex resource management issues: the Water-Food-Energy Nexus Game (Nexus Game); the Narubu Game of Many Voices (Narubu Game); and the Forest Governance Game (Forest Game). The games contain instructive and reflexive mechanisms that prompt players to self-discover common challenges associated with complex nexus issues, including conflicting institutional mandates, social dilemmas, contending worldviews, and plural interpretations of science

Genes and (auto)immunity in primary biliary cirrhosis

Primary biliary cirrhosis (PBC) is a chronic autoimmune cholestatic liver disease most commonly encountered in postmenopausal women; it is characterized by high-titer serum autoantibodies to mitochondrial antigens, elevated serum IgM, progressive destruction of intrahepatic bile ducts, and ultimately liver cirrhosis and failure. The cytopathic mechanisms leading to the selective destruction of intrahepatic cholangiocytes are still largely unknown. The current theory on the pathogenesis of PBC indicated that environmental factors might trigger autoimmunity in genetically susceptible individuals. In fact, genetic predisposition is crit. to disease onset and progression, yet peculiar among autoimmune diseases, as indicated by the lack of a strong assocn. with major histocompatibility complex haplotypes. Further, the recently reported concordance rate among monozygotic twins strenghtens the importance of genetic factors, while also indicating that addnl. factors, possibly infectious agents or xenobiotics, intervene to trigger the disease. In this review, the available data regarding the genetic factors assocd. with PBC susceptibility and progression, as well as the available evidence regarding the immunomediated pathogenesis of PBC, will be critically illustrated and discussed

Autoimmune hepatitis: new paradigms in the pathogenesis, diagnosis, and management

Autoimmune hepatitis (AIH), primary biliary cirrhosis, and primary sclerosing cholangitis are the three major autoimmune diseases affecting the liver, and of these three, AIH is the most typical autoimmune disease being characterized by a T-cell-rich infiltrate, raised circulating γ-globulins, autoantibodies, HLA associations, and links with other autoimmune diseases. It is the only one, of the three diseases, that responds well to immunosuppressive therapy. AIH is caused by dysregulation of immunoregulatory networks and the consequent emergence of autoreactive T cells that orchestrate a progressive destruction of hepatocytes leading untreated to liver failure. T cells play a major role in the immunopathogenesis, and both CD4+ and CD8+ T cells are involved together with effector responses mediated by NK cells, γδ T cells, and macrophages. A number of triggering factors have been proposed including viruses, xenobiotics, and drugs, but none have been conclusively shown to be involved in pathogenesis