Biomass production and land use management in the Italian context: regulations, conflicts, and impacts

Renewable energy sources, such as biomass can make a positive impact on climate change phenomenon by decreasing our dependence on fossil fuels. The use of biomass energy is directly linked to the use of the land, from which biomass feedstock is obtained, such as farm land and forests, and its ecosystem services. The biomass production and the use of land and ecosystem services are usually associated with a wide range of environmental and social impacts, depending on what choices are made regarding what types of biomass are used, as well as where and how they are produced. Choosing management practices that minimize negative impacts and complement planning policies and energy production objectives is often associated with land-use conflicts among both different institutional levels, local, national and European, and different social actors. Yet, European Directive 2009/28/CE establishes that the energy production from renewable energy by 2020, as well as from biofuel, defined for each member state (Annex 1), must be achieved through a “sustainable†production. Such definition is assigned to national and local contexts, arising issues in policy making, conflicts analysis and methodologies. The present paper discusses on the recent acknowledgment of the above mentioned EU directive in several Italian Regions, such as Puglia and Marche, which have defined regulations/guidelines regarding their potential contribution to the national objectives of production and consumption of energy from renewable sources (EFR). Moreover, the present paper confronts such regulations with results found in literature. Several analyses have been done on the energy production from biomass based on technical and economic aspects of the problem. However, few studies have applied integrated approaches able to take into consideration crucial aspects such as biodiversity conservation and landscape fragmentation, as required by EU Directive 2009/28/CE, side by side with the economic and social dimensions. This paper aims at filling this gap proposing the application of an integrated framework of analysis, based on multi-criteria approaches able to take into consideration socio-economic, environmental and landscape criteria, as well as institutional and social conflicts linked to the biomass production.

Italian hybrid fire prevention code

Fire safety of residential buildings and activities subjected to fire inspection is a difficult task, especially when the safety targets have to be adopted in built buildings or in activities that are going to be modified into more complex ones. Generally, these circumstances show more constraints and it could be difficult to achieve an acceptable level of fire residual risk by prescriptive based fireregulations. Therefore, the Italian National Fire Rescue and Service in charge for fire safety, in August 2015 issued a new Fire Prevention Code whose design methodology is more oriented to fire performance based design rather than prescriptive fire codes. The flexibility of this new fire design methodology offers a very complex tool to experts in order to design fire safety measures and strategies of buildings and activities subjected to fire inspection. The present paper aims tohighlig hts the contents and the fire safety strategy design methodology of the new Italian Fire Prevention Code

Challenges of implementing maritime spatial planning in the Eastern Mediterranean

The Maritime Spatial Planning Directive (2014/89/EU) was launched to create a common framework for maritime spatial planning (MSP) in Europe. The case of the Adriatic and Ionian Region (AIR) is emblematic of the peculiar challenges to be faced while implementing MSP in the region

Migración haitiana en Santiago de Chile: Expulsiones, imaginarios e inserción social en un Estado-nación neoliberal

The objective of this article is to characterize and analyze Haitian migration in Chile, in the global context in which it occurs, addressing it from a micro and meso level, understanding from its subjectivities the vulnerable relationships established with the different actors with which they interact in life daily and the role they play in the gradual transformation of social and work spaces. The type of research is qualitative, through the ethnographic method. It is observed that Haitian migration is a challenge for the Chilean State in terms of demand for services and coexistence, located in the field of economic, social, political and cultural rights. Particularly, Haitian migration anthropologically questions the idea of Chilean spatial, temporal and identity boundaries, offering various plans on the possibility of improving their lives and overcoming the barriers they face in a neo-liberal society. It is concluded that Haitians tend to be racialized and excluded, being subalternized, however, to the extent that they manage to work in a stable manner, they are inserted and even generate a willingness to take root considering that the possibility of returning to Haiti is quite uncertain, given the serious political-economic problems from your country of origin.El objetivo del presente artículo es caracterizar y analizar la migración haitiana en Chile, en el contexto global en que ocurre, abordándolo desde un nivel micro y meso, comprendiendo desde sus subjetividades las vulnerables relaciones que establecen con los distintos actores con que interactúan en la vida cotidiana y el papel que juegan en la paulatina transformación de espacios sociales y laborales. El tipo de investigación es cualitativo, a través del método etnográfico. Se observa que la migración haitiana en un desafío para el Estado chileno en términos de demanda de servicios y convivencia, ubicado en el ámbito de los derechos económicos, sociales, políticos y culturales. Particularmente la migración haitiana cuestiona antropológicamente la idea de los límites espaciales, temporales e identitarios chilenos, ofreciendo diversos planos sobre la posibilidad de mejorar su vida y superar las barreras que enfrentan en una sociedad neoliberal. Se concluye que los/as haitianos/as tienden a ser racializados y excluidos, quedando subalternizados, sin embargo, en la medida que logran trabajar de manera estable, se insertan e incluso generan una disposición al arraigo considerando que la posibilidad del regreso hacia Haití es bastante incierta, dados los graves problemas político-económicos de su país de origen

Comparative genomics provides an operational classification system and reveals early emergence and biased spatio-temporal distribution of SARS-CoV-2

Effective systems for the analysis of molecular data are of fundamental importance for real-time monitoring of the spread of infectious diseases and the study of pathogen evolution. While the Nextstrain and GISAID portals offer widely used systems for the classification of SARS-CoV-2 genomes, both present relevant limitations. Here we propose a highly reproducible method for the systematic classification of SARS-CoV-2 viral types. To demonstrate the validity of our approach, we conduct an extensive comparative genomic analysis of more than 20,000 SARS-CoV-2 genomes. Our classification system delineates 12 clusters and 4 super-clusters in SARS-CoV-2, with a highly biased spatio-temporal distribution worldwide, and provides important observations concerning the evolutionary processes associated with the emergence of novel viral types. Based on the estimates of SARS-CoV-2 evolutionary rate and genetic distances of genomes of the early pandemic phase, we infer that SARS-CoV-2 could have been circulating in humans since August-November 2019. The observed pattern of genomic variability is remarkably similar between all clusters and super-clusters, being UTRs and the s2m element, a highly conserved secondary structure element, the most variable genomic regions. While several polymorphic sites that are specific to one or more clusters were predicted to be under positive or negative selection, overall, our analyses also suggest that the emergence of novel genome types is unlikely to be driven by widespread convergent evolution and independent fixation of advantageous substitutions. While, in the absence of rigorous experimental validation, several questions concerning the evolutionary processes and the phenotypic characteristics (increased/decreased virulence) remain open, we believe that the approach outlined in this study can be of relevance for the tracking and functional characterization of different types of SARS-CoV-2 genomes

The mitochondrial genome of Phallusia mammillata and Phallusia fumigata (Tunicata, Ascidiacea): high genome plasticity at intra-genus level

Background: Within Chordata, the subphyla Vertebrata and Cephalochordata (Iancelets) are characterized by a remarkable stability of the mitochondrial (mt) genome, with constancy of gene content and almost invariant gene order, whereas the limited mitochondrial data on the subphylum Tunicata suggest frequent and extensive gene rearrangements, observed also within ascidians of the same genus. Results: To confirm this evolutionary trend and to better understand the evolutionary dynamics of the mitochondrial genome in Tunicata Ascidiacea, we have sequenced and characterized the complete mt genome of two congeneric ascidian species, Phallusia mammillata and Phallusia fumigata (Phlebobranchiata, Ascidiidae). The two mtDNAs are surprisingly rearranged, both with respect to one another and relative to those of other tunicates and chordates, with gene rearrangements affecting both protein-coding and tRNA genes. The new data highlight the extraordinary variability of ascidian mt genome in base composition, tRNA secondary structure, tRNA gene content, and non-coding regions (number, size, sequence and location). Indeed, both Phallusia genomes lack the trnD gene, show loss/acquisition of DHU-arm in two tRNAs, and have a G+C content two-fold higher than other ascidians. Moreover, the mt genome of P. fumigata presents two identical copies of trnI, an extra tRNA gene with uncertain amino acid specificity, and four almost identical sequence regions. In addition, a truncated cytochrome b, lacking a C-terminal tail that commonly protrudes into the mt matrix, has been identified as a new mt feature probably shared by all tunicates. Conclusion: The frequent occurrence of major gene order rearrangements in ascidians both at high taxonomic level and within the same genus makes this taxon an excellent model to study the mechanisms of gene rearrangement, and renders the mt genome an invaluable phylogenetic marker to investigate molecular biodiversity and speciation events in this largely unexplored group of basal chordates

Huntingtin gene evolution in Chordata and its peculiar features in the ascidian Ciona genus

BACKGROUND: To gain insight into the evolutionary features of the huntingtin (htt) gene in Chordata, we have sequenced and characterized the full-length htt mRNA in the ascidian Ciona intestinalis, a basal chordate emerging as new invertebrate model organism. Moreover, taking advantage of the availability of genomic and EST sequences, the htt gene structure of a number of chordate species, including the cogeneric ascidian Ciona savignyi, and the vertebrates Xenopus and Gallus was reconstructed. RESULTS: The C. intestinalis htt transcript exhibits some peculiar features, such as spliced leader trans-splicing in the 98 nt-long 5' untranslated region (UTR), an alternative splicing in the coding region, eight alternative polyadenylation sites, and no similarities of both 5' and 3'UTRs compared to homologs of the cogeneric C. savignyi. The predicted protein is 2946 amino acids long, shorter than its vertebrate homologs, and lacks the polyQ and the polyP stretches found in the the N-terminal regions of mammalian homologs. The exon-intron organization of the htt gene is almost identical among vertebrates, and significantly conserved between Ciona and vertebrates, allowing us to hypothesize an ancestral chordate gene consisting of at least 40 coding exons. CONCLUSION: During chordate diversification, events of gain/loss, sliding, phase changes, and expansion of introns occurred in both vertebrate and ascidian lineages predominantly in the 5'-half of the htt gene, where there is also evidence of lineage-specific evolutionary dynamics in vertebrates. On the contrary, the 3'-half of the gene is highly conserved in all chordates at the level of both gene structure and protein sequence. Between the two Ciona species, a fast evolutionary rate and/or an early divergence time is suggested by the absence of significant similarity between UTRs, protein divergence comparable to that observed between mammals and fishes, and different distribution of repetitive elements

Spread of the non-indigenous ascidian Aplidium accarense (Millar, 1953) in the Eastern Mediterranean Sea: morphological and molecular tools for an accurate identification

The aplousobranch ascidian Aplidium accarense (Millar, 1953) was first described on the western coast of Africa, where it is considered native. Afterwards, this species was introduced along south-American Atlantic coasts, where it affected local shellfish farms through a massive colonization of both natural and artificial substrata. Aplidium accarense has been recently reported along Catalan coasts and in the Tyrrhenian Seas (Western Mediterranean) where it represents a non-indigenous species, only recorded in harbours and aquaculture farms thus far. These Mediterranean records support the hypothesis that A. accarense is currently expanding within the basin, representing a potential invasive species. In this study, several colonies of A. accarense were found for the first time on artificial substrata within the semi-enclosed basin of the Mar Piccolo of Taranto (Italy, Ionian Sea), in the Eastern Mediterranean. Here we provide an updated description of A. accarense combining both morphological and molecular approaches, in order to allow an accurate and reliable identification of this expanding species. Comparing the morphology of the specimens collected from Taranto with the previous descriptions, a slight intra-specific variability has been noticed. Therefore, we provide detailed comparisons of the specimens found in Taranto with all the other A. accarense sampled in other areas of the world, in order to highlight the intra-species variability. The correct identification of a potentially-dangerous species such as A. accarense, represents a needed step for environmental monitoring purposes and for implementing management strategies to mitigate the effects of non-indigenous species on natural ecosystems and human activities

Glutamine synthetase gene evolution in bacteria.

The evolution of the prokaryotic glutamine synthase (GS) genes, namely the GSI and GSII isoforms, has been investigated using the second codon positions, which have previously proven to behave as a good molecular clock. Our data confirm the early divergence between prokaryotic and eukaryotic GSII before the splitting between plants and animals. The phylogenetic tree of the GSI isoforms shows Archaebacteria to be more closely related to Eubacteria than to Eukaryotes. This finding is confirmed by the phylogenetic analysis carried out on both large and small subunits of rRNA. However, differently from the rRNA analyses, Crenarchaeota and Euryarchaeota Archaebacteria, as well as high- and low-GC gram-positive bacteria, appear to be polyphyletic. We provide evidence that the observed polyphyly of Archaebacteria might be only apparent, resulting from a gene duplication event preceding the split between Archaebacteria and Eubacteria and followed by the retention of only one isoform in the extant lineages. Both gram-negative bacteria and high-GC gram-positive bacteria, which appear closely related, have GS activity regulated by an adenylylation/deadenylylation mechanism. A lateral gene transfer from Archaebacteria to low-GC eubacteria is invoked to explain the observed polyphyly of gram-positive bacteria

Untranslated regions of mRNAs

Gene expression is finely regulated at the post-transcriptional level. Features of the untranslated regions of mRNAs that control their translation, degradation and localization include stem-loop structures, upstream initiation codons and open reading frames, internal ribosome entry sites and various cis-acting elements that are bound by RNA-binding proteins