Seurat-1: HepaRG, repeated and single dose exposure for Mitochondrial Health and LipidTox

The purpose of this report is to describe the experimental procedure used in order to detect changes in mitochondrial membrane potential and lipid accumulation following exposure of HepaRG cells to various chemicals both by repeated exposure as single exposure to chemicals. This procedure was created for the SEURAT-1 Project runs 15 to 18 and was developed by using live cell imaging.JRC.I.5-Systems Toxicolog

A high throughput imaging database of toxicological effects of nanomaterials tested on HepaRG cells

The large amount of existing nanomaterials demands rapid and reliable methods for testing their potential toxicological effect on human health, preferably by means of relevant in vitro techniques in order to reduce testing on animals. Combining high throughput workflows with automated high content imaging techniques allows deriving much more information from cell-based assays than the typical readouts (i.e. one measurement per well) with optical plate-readers. We present here a dataset including data based on a maximum of 14 different read outs (including viable cell count, cell membrane permeability, apoptotic cell death, mitochondrial membrane potential and steatosis) of the human hepatoma HepaRG cell line treated with a large set of nanomaterials, coatings and supernatants at different concentrations. The database, given its size, can be utilized in the development of in silico hazard assessment and prediction tools or can be combined with toxicity results from other in vitro test systems.peer-reviewe

Les formes sociales de l'équilibre alimentaire (du repas méridien à la journée alimentaire)

Bureau de recherches géologiques et minières - Orléans (brgm) / SudocSudocFranceF

Three-dimensional automated nanoparticle tracking using Mie scattering in an optical microscope

The forward scattering of light in a conventional inverted optical microscope by nanoparticles ranging in diameter from 10 to 50nm has been used to automatically and quantitatively identify and track their location in three-dimensions with a temporal resolution of 200ms and a spatial precision of about 5nm. The microscope objective was oscillated along the light path using a piezo actuator and images acquired with the condenser aperture closed to a minimum to enhance the effects of diffraction. Data processing in the time and spatial domains allowed the location of particles to be obtained automatically so that the technique has applications both in the processing of nanoparticles and in their use in a variety of fields including nanobiotechnology, pharmaceuticals and food processing where a simple optical microscope maybe preferred for a variety of reasons.JRC.I.6-Systems toxicolog

Tracking nanoparticles optically to study their interaction with cells

Nanoparticles are by definition too small to be visible in an optical microscope and devices such as scanning electron microscopes must be used to resolve them. However electron beams quickly lead to cell death and so it is difficult to study the interaction of nanoparticles with living cells in order to establish whether such interactions could be damaging to the cell. A simple modification to a conventional inverted optical microscope is proposed here which renders the location of nanoparticles readily apparent and permits tracking of them in three-dimensions. Particles in the range 100nm to 500nm have been tracked with a temporal resolution of 200ms. The technique, although motivated by the desire to study the interaction of nanoparticles with cells, has a wide range of potential applications in the fields of food processing, pharmaceuticals and nano-biotechnology.JRC.I.5-Systems Toxicolog

DETAILED PERFORMANCE ANALYSIS OF THE 10KW CNRS-PROMES DISH/STIRLING SYSTEM

The campaign to measure the optical and thermodynamic efficiency of the system will be presented. The optical quality was determined with a classical flux-mapping using a lambertian target and a CCD camera system, which also leads to the energy input to the PCU. For the thermodynamic analysis all the data necessary for a complete energy balance around the Stirling engine, i.e. efficiency of the Stirling motor, the cavity and the receiver as well as the parasitic losses were measured. Such a detailed performance analysis helps to quantify all significant losses of the system and to identify the most rewarding future improvements

Eurodish-Dauerbetrieb, Weiterentwicklung und Referenzanlagen

Das 10 kWel Dish/Stirling-System EuroDish wurde überarbeitet. Verschiedene Bauteile wurden neu entwickelt und verbessert wie die hoch effiziente und standfeste keramische Cavity-Isolierung und die um zahlreiche Funktionen erweiterte Steuerung. Der Konzentrator wurde in einem neuartigen Verfahren montiert, das die optische Genauigkeit erhöht. In Spanien, Frankreich und Deutschland wurden Referenzanlagen gebaut und werden seither kontinuierlich betrieben. Die Leistungsausbeute und die Systemverfügbarkeit konnten erhöht werden, die Möglichkeiten zur Anlagenüberwachung und Betriebsanalyse wurden wesentlich verbessert

Detailed Performance Analysis of a 10 kW Dish/Stirling System

The CNRS-Promes dish/Stirling system was erected in Jun. 2004 as the last of three country reference units built in the “Envirodish” project. It represents the latest development step of the EuroDish system with many improved components. With a measured peak of 11 kW electrical output power, it is also the best performing system so far. The measurement campaign to determine the optical and thermodynamic efficiency of the system is presented. The optical quality of the concentrator and the energy input to the power conversion unit was measured with a classical flux-mapping system using a Lambertian target and a charge coupled device camera system. An efficiency of the concentrator including the intercept losses of 74.4% could be defined for this particular system. For the thermodynamic analysis all the data necessary for a complete energy balance around the Stirling engine were measured or approximated by calculations. For the given ambient conditions during the tests, a Stirling engine efficiency of 39.4% could be measured. The overall efficiency for the conversion of solar to electric energy was 22.5%

A Heterodyne Mach-Zehnder Interferometer, employing static and dynamic phase demodulation techniques for live-cell imaging

This paper describes a temporal carrier based Heterodyne Interferometer and associated phase demodulation techniques which are suitable for phase imaging of live cells. A Mach-Zehnder Interferometer is integrated to the microscope and two acousto-optic modulators are employed, to generate a temporal carrier that allows heterodyne approach to phase demodulation. Two demodulation schemes are presented: (a) Digital heterodyne phase extraction technique to extract the static phase information of the carrier signal, and (b) dynamic phase extraction technique for extracting phase variation in the carrier signal. The Heterodyne interferometer enables fast phase imaging and coupled with digital heterodyne phase extraction process, the system provides excellent temporal phase stability (standard deviation < 2 nm for 16 second measurement). This technique is employed for quantitative phase imaging of 3T3 fibroblast cells immersed in cell media. When there is phase variation, the temporal carrier signal is modulated and its instantaneous frequency is directly related to the variation. The dynamic phase extraction technique first determines the instantaneous frequency, which is then integrated with respect to time to obtain timevarying phase. The algorithm is able to extract a time varying phase, caused by a stimulated vibration at 30 Hz and 40 nm amplitud