A Role for Bottom-Up Synthetic Cells in the Internet of Bio-Nano Things?

he potential role of bottom-up Synthetic Cells (SCs) in the Internet of Bio-Nano Things (IoBNT) is discussed. In particular, this perspective paper focuses on the growing interest in networks of biological and/or artificial objects at the micro- and nanoscale (cells and subcellular parts, microelectrodes, microvessels, etc.), whereby communication takes place in an unconventional manner, i.e., via chemical signaling. The resulting “molecular communication” (MC) scenario paves the way to the development of innovative technologies that have the potential to impact biotechnology, nanomedicine, and related fields. The scenario that relies on the interconnection of natural and artificial entities is briefly introduced, highlighting how Synthetic Biology (SB) plays a central role. SB allows the construction of various types of SCs that can be designed, tailored, and programmed according to specific predefined requirements. In particular, “bottom-up” SCs are briefly described by commenting on the principles of their design and fabrication and their features (in particular, the capacity to exchange chemicals with other SCs or with natural biological cells). Although bottom-up SCs still have low complexity and thus basic functionalities, here, we introduce their potential role in the IoBNT. This perspective paper aims to stimulate interest in and discussion on the presented topics. The article also includes commentaries on MC, semantic information, minimal cognition, wetware neuromorphic engineering, and chemical social robotics, with the specific potential they can bring to the IoBNT

Molecular characterization of ‘Candidatus Phytoplasma prunorum’ in Cacopsilla pruni insect vector

Recent investigations on molecular characterization of the ‘Candidatus Phytoplasma prunorum’ (16SrX-B subgroup), causal agent of the European Stone Fruit Yellows (ESFY) syndrome, on the non ribosomal tuf gene resulted in the finding of two groups of isolates, named ‘type a’ and ‘type b’, both with a distinctive geographical distribution in Italian stone fruit growing areas (Ferretti et al., 2007 and 2008). Considering the role of Cacopsylla pruni (Scopoli) in the epidemiological cycle of the disease, the presence of the two groups of isolates has also been investigated in infected psyllid individuals from different Italian areas. Both types have been identified in C. pruni specimens collected on apricot, plum and wild Prunus species, confirming the geographical distribution and the percentages of spread of the two isolates.Keywords: ESFY, phytoplasma, characterization, tuf gene, insect vecto