PURIFICATION AND BIOLOGICAL ACTIVITIES OF COMBINATION OF HEMI-SYNTHESIZED THIOSEMICARBAZONES OF MITRACARPUS SCABER ZUCC

Objective: The harvested Mitracarpus Scaber plants are identified in the national herbarium and registered under the number AA. 6252/HLB. During this work, three crude extracts are prepared from three organic solvents, namely dichloromethane; ethanol and hydroethanol in 50/50 v/v proportion. Methods: The respective alkaloid extracts obtained from the corresponding crude extracts served as substrates for the hemi synthesis of thiosemicarbazone totals from thiosemicarbazides. The three hemi-synthesis products obtained were tested on eight (08) strains of germs, namely E. coli ATCC 25922, S. aureus ATCC 25923, E. faecalis ATCC 22921, P. aeruginosa ATCC 27853, C. albicans ATCC 10231, S. typhi, K. pneumoniae and Dermatophilus 146. Results: The ethanolic extract of thiosemicarbazones exhibited the best bioactive activity and was found to be the most selective. By a series of bioguided chromatographies: TLC thin layer chromatography; CPA atmospheric pressure chromatography and medium pressure liquid chromatography. MPLC (medium pressure liquid chromatography), separation on dextran gel: Sephadex® LH20). The use of available spectral data, cross-checked with that of the literature, made it possible to identify and purify three (03) molecules of thiosemicarbazones, making it possible to study the biological activity of their combination. Conclusion: On the basis of results, this work has made it possible to confirm the concept which affirms that medicinal plants are libraries of several thousand molecules.                             Peer Review History: Received 2 December 2019;   Revised 20 December; Accepted 5 January, Available online 15 January 2020 Academic Editor: Ahmad Najib, Universitas Muslim Indonesia,  Indonesia, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, [email protected] Prof. Dr. Amani S. Awaad, Prince Sattam Bin Abdulaziz University, Al-Kharj. KSA., [email protected] Similar Articles: ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS ANTI-INFLAMMATORY AND ANTI-OXIDANT ACTIVITIES OF METHANOL EXTRACT OF BAPHIA NITID

Perception des producteurs et impact des facteurs socio-économiques sur la connaissance des mycotoxines du maïs en stockage au Bénin

Au Bénin, plusieurs technologies sont utilisées pour la conservation du maïs. Ces technologies utilisées ne contrôlent pas toujours les pertes fongiques enregistrées. L’objectif de l’étude est d’évaluer l’impact des facteurs socio-économiques des producteurs sur les technologies de prévention des pertes fongiques et leur niveau de connaissance des mycotoxines du maïs en stockage. Pour ce faire, une enquête a été réalisée dans sept zones agro-écologiques du Bénin. Au total, sept technologies de stockages du maïs ont été inventoriées (de A à G). La technologie B est non seulement présente dans toutes les zones parcourues mais aussi, elle est fortement représentée dans les zones III, IV et VIII avec des proportions respectives de 51,33%; 62,86% et 69,83%. La population agricole ayant un niveau secondaire (27%) et les alphabétisés (15%) ont la chance de connaître les mycotoxines. Les enquêtés (100%) ne connaissent pas le danger lié à la consommation du maïs contaminé et n’ont jamais reçu une formation sur les mycotoxines. L’observation de la couleur verte (61% des enquêtés) et la couleur noire (28% des enquêtés) témoigne bien de la persistance des mycotoxines dans le maïs en stock au Bénin.© 2016 International Formulae Group. All rights reserved.Mots clés: Technologie, conservation, céréales, contamination, moisissuresEnglish Title: Perception of maize producers in Benin and impact of the socio-economic factors on the knowledge of the mycotoxins of maize in storageEnglish AbstractIn Benin, various technologies are used for the conservation of the maize. Those technologies don't often control the recorded fungal losses. The objective of the study is to value the impact of the socioeconomic factors of producers on technologies of fungal loss prevention and their level of knowledge of mycotoxins of the maize in storage. A survey has been achieved in seven agro - ecological zones of Benin. Thus, a total of seven maize storage technologies were inventoried (A to G). The use of technology B is not only apparent in all the zones covered but it is also significantly used in zones III, IV and VIII in the respective proportions of51.33%; 62.86% and 69.83%. The agricultural population, i.e. those with secondary education (27%) and the literates (15%) happen to have some knowledge about mycotoxins. People investigated (100%) do not have knowledge of the danger associated with the consumption of contaminated maize and have never received training on mycotoxins. The observation of a green colour (by 61% of respondents) and black colour (by 28% of respondents) is testimony to the persistence of mycotoxins in maize under storage in Benin.© 2016 International Formulae Group. All rights reserved.Keywords: Technology, conservation, cereals, contamination, mould

IDENTIFICATION AND QUANTIFICATION OF PHOSPHODIESTERASE-5 INHIBITOR AS FALSIFIED IN ‘‘NATURAL’’ MALAYSIAN HERBAL APHRODISIACS SOLD IN SOME BENINESE MARKETS

Objective: Nowadays, there have been several reports of herbal products falsified with well-known synthetic molecules, leading to harmful health consequences for the consumer. The aim of this study was to assess the profile of ‘natural' herbal aphrodisiacs in the local markets of the municipalities of Cotonou and Abomey-Calavi in Benin and to screen some of them for the presence of additives such as sildenafil and tadalafil. Methods: A non-probability survey was conducted to identify the available aphrodisiacs and their characteristics. Some of them were then selected for analysis. Thin Layer Chromatography (TLC) was adopted for qualitative detection. The TLC positive extracts were then analyzed by HPLC on a C18 column with a mobile phase consisting of a mixture of 0.05M phosphate buffer (pH 5.8), acetonitrile and methanol (30:50:20). The Detection was performed at 290 nm. Results: Seventy-seven aphrodisiacs were identified and from these, 18 were selected for analysis. Six of them were adulterated with tadalafil. The concentration of tadalafil in the samples was 1.7 to 4.6 times higher than the recommended dose of 20 mg. Conclusion: This work opens the door to the need to control ‘‘natural’’ labeled products in order to ensure their quality

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY QUANTIFICATION OF OLEANOLIC ACID IN LAUNAEA TARAXACIFOLIA AND LARVICIDAL ACTIVITY AGAINST ANOPHELES GAMBIAE

Objective: One of the measures used to prevent malaria is the management of breeding sites. For preventive and ecologically profitable control, the use of bio-larvicides made from active plant extracts would be an asset for the control of malaria vectors, in particular Anopheles gambiae. Advances in pharmacognosy have revealed the benefits of several phytochemicals with very rich and varied therapeutic effects. Among the latter, oleanolic acid (OA) is quite remarkable because of its various and multiple properties, much of which is demonstrated with the leaves of Launaea taraxacifolia. Methods: After a liquid-liquid fractionation with different organic solvents of the hydro-methanolic extract of Launaea taraxacifolia, we obtained three fractions named Fhex (hexane fraction), FDCM (dichloromethane fraction) and FHM (hydro-methanolic fraction) which were tested on 3rd instar Anopheles gambiae larvae. Results: Fhex proved to be the most active with LC50 of 120.11 ppm and 69.50 ppm respectively in 24 and 48 hours of contact. We then developed a new method of Ultra-Violet High Performance Liquid Chromatography (HPLC / UV) method and determined the quantity of oleanolic acid in the Fhex and FDCM fractions to be respectively 0.46% and 0.23% . Conclusion: Launaea taraxacifolia has a larvicidal potential due to the presence of oleanolic acid whose inhibitory effect against Anopheles gambiae larvae

TOXICITY AND ANTITRYPANOSOMAN ACTIVITY OF HEMI SYNTHESIS PRODUCTS OBTAINED FROM BIOACTIVE COMPOUNDS OF MITRACARPUS SCABER HARVESTED SOUTH OF BENIN

Objective: Present work involve study of the toxicity and the antitrypanosomal activity of the hemi syntheses (total of thiosemicarbazones) extracted from the substrates (reagents of thiosemicarbazides) in 1N hydrochloric acid medium from Mitracarpus Scaber harvested in the region of Abomey-calavi. Methods: The ethanolic, dichloromethane and hydroethanol extracts (50:50 v/v) yielded the alkaloid extracts with yields of 4.44%, 2.48% and 5.08% respectively leading to three products of hemi-synthesis P1, P2 and P3 whose larval toxicities have respective values LC50: 78.1μg/mL, 95μg/mL, and 48.8 μg/mL whereas the toxicity tests of the alkaloid samples of ethanol, dichloromethane and hydroethanolic E1, E2 and E3 extracted from the evaluation of the same larval toxicity test gave values of 83.41μg/mL 102.51μg/mL and 52.91μg/mL respectively. Results: Results were less toxic than those of semisynthetic products. Acute and sub-acute toxicity in non-pregnant NMRI female mice after oral gavage of P2 product has been shown to be non-toxic. The antitrypanosomal test was carried out according to the Alamar blue method, it revealed that P1 moderately inhibits trypanosome parasites (IC50=18.06 μg/mL) as well as P2 and P3 with a respective IC50 of 17.24 μg/mL and 20, 68 μg/mL while the alkaloid totals had lower antitrypanosomal activity than the hemi-synthesis products. Conclusion: Study concludes that, shrimp larvae were tolerant of ethanolic, dichloromethane and hydroethanolic extracts and therefore the leafy stems of the plant do not have a priori harmful effects on human cells 9 PS, 9 KB, A-549 and HT-29.                       Peer Review History: Received 12 October 2019;   Revised 28 October; Accepted 5 November, Available online 15 November 2019 Academic Editor: Ahmad Najib, Universitas Muslim Indonesia,  Indonesia, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.5/10 Reviewer(s) detail: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt, [email protected]  Dr. Dalia Kamal Zaffar Ali, Modern University for technology and information, Egypt, [email protected] Similar Articles: LEAD ACETATE: A DANGEROUS ENVIRONMENTAL TOXICANT THAT IS AMELIORATED BY THE AQUEOUS EXTRACT OF FICUS VOGELII (FV

CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OILS OF FOUR VARIETIES OF LIPPIA MULTIFLORA IN BENIN

Objective: Present study involves the study of the chemical composition of the essential oils extracted from the leaves by gas chromatography and gas chromatography coupled with mass spectrometry of Lippia multiflora harvested in the regions of Kétou, Savalou, Bohicon and Mono and tested by the well diffusion method against pathogenic microorganisms.Methods: The essential oils studied are terpene compounds, aromatic compounds, aliphatic compounds and other natural substances. The inhibition zone diameters determined allowed us to evaluate their degree of germ sensitivity of the strains tested. Essential oils extracted from Lippia multiflora harvested in these areas have the most pronounced antimicrobial activity.Results: In total, the essential oils tested have different and specifically a degree of sensitivity against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis A24974, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa except that harvested in the Savalou who does not have no degree of sensitivity on Pseudomonas aeruginosa.Conclusion: This work paves the way for food preservation with extracted natural substances and also the formulation of natural antimicrobials for this fact.                       Peer Review History: Received 12 July 2019;   Revised 9 August; Accepted 1 September, Available online 15 September 2019 Academic Editor: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt, [email protected]  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 7/10 Average Peer review marks at publication stage: 9.4/10 Reviewer(s) detail: Rola Jadallah, Arab American University, Palestine, [email protected] Dr. Md. Shahidul Islam, USTC, Chittagong, Bangladesh, [email protected] Similar Articles: ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF PSIDIUM GUAJAVA. (GUAVA) MEDICINAL PLANT LEAVES USED IN FOLK MEDICINE FOR TREATMENT OF WOUNDS AND BURNS IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEMEN IN-VITRO ANTIBACTERIAL ACTIVITY OF LACTOBACILLI METABOLITES LOADED HYDROGEL FORMULATIONS AGAINST PSEUDOMONAS AERUGINOS

Maize Fungal Growth Control with Scopoletin of Cassava Roots Produced in Benin

The chemical contamination of food is among the main public health issues in developing countries. With a view to find new natural bioactive products against fungi responsible for chemical contamination of staple food such as maize, the antifungal activity tests of scopoletin extracted from different components of the cassava root produced in Benin were carried out. The dosage of scopoletin from parts of the root (first skin, second skin, whole root, and flesh) was done by High Performance Liquid Chromatography. The scopoletin extract was used to assess the activity of 12 strains (11 strains of maize and a reference strain). The presence of scopoletin was revealed in all components of the cassava root. Scopoletin extracted from the first skin cassava root was the most active both as inhibition of sporulation (52.29 to 87.91%) and the mycelial growth (36.51–80.41%). Scopoletin extract from the cassava root skins showed significant inhibitory activity on the tested strains with fungicide concentration (MFC) between 0.0125 mg/mL and 0.1 mg/mL. The antifungal scopoletin extracted from the cassava root skins may be well beneficial for the fungal control of the storage of maize

Diet supplement effect based on cottonseed meal and Vitellaria paradoxa leaves on Borgou cows performance in rainy season

An experiment was carried out to investigate the effect of supplementing lactating cows with cottonseed meal and Vitellaria paradoxa leaves in the rainy season on milk yield and content, cows daily weight gain and profitability. The experimental design was 3 × 3 Latin square with 5 repetitions. Fifteen Borgou cows were offered three diets: grazing on natural pasture, grazing on pasture plus 1.5 kg of cottonseed meal supplement and grazing on natural pasture plus 500 g of Vitellaria paradoxa leaves. Daily milk yield was 946.58 g, 1690.07 g and 1176.89 g for the control, cottonseed meal and Vitellaria paradoxa leaves group respectively. Cows supplemented with cottonseed meal produce significantly (p < 0.05) more milk than the others. The mean values were 15.12%, 0.35%, 5.92% and 4.13% respectively for total solid, ash, fat and protein content. Vitellaria paradoxa leaves significantly (p < 0.05) increase total solid level and ash as well as ash with cottonseed meal. Furthermore, calves daily weight gain (DWG) was significantly different. A net return analysis shows that cottonseed meal and Vitellaria paradoxa leaves supplementation in rainy season was profitable even if Vitellaria paradoxa leaves were more beneficial