Applications of Differential Subordination and Superordination

In the present investigation we obtain asndwich theorems for certain subclass of analytic functions defined by convolution. Our results generalizes several well known results.DOI : http://dx.doi.org/10.22342/jims.14.1.62.47-5

Growth of large-sized relaxor ferroelectric PZN-PT single crystals by modified flux growth method

A novel bottom-cooling high-temperature solution growth technique is developed for growing large-sized relaxor ferroelectric 0.91Pb(Zn1/3Nb2/3O3)-0.09PbTiO3 (PZN-PT) single crystals. During the growth, an inverse temperature gradient is maintained in the crucible base by flowing air at a controlled rate. This method restricts the number of spontaneously nucleated crystals at crucible bottom, reduces loss of volatile PbO component and favours the growth of large-sized PZN-PT single crystals. Large-sized PZN-PT single crystals of dimensions ~ 22x20x14 mm3 are reproducibly grown by the proposed method. The electrical characteristics of the PZN-PT wafers oriented along the , and directions are investigated. PZN-PT wafers oriented along the direction exhibited superior piezoelectric coefficient (d33) of ~ 2221 pm/V. The homogeneity of the physical parameters is analysed by preparing 10 elements with dimensions of ~5x2.5x2.5 mm3 which were cut from single wafer oriented along the direction. The ferro-, piezo- and dielectric characteristics of these wafers were found to be highly uniform with small standard deviation. The observation of d33 value with less than 2 % deviation from mean value confirms the growth of high quality PZN-PT single crystals.Comment: 16 pages, 7 figure

A Genotype-First Approach for the Molecular and Clinical Characterization of Uncommon De Novo Microdeletion of 20q13.33

Background: Subtelomeric deletions of the long arm of chromosome 20 are rare, with only 11 described in the literature. Clinical features of individuals with these microdeletions include severe limb malformations, skeletal abnormalities, growth retardation, developmental and speech delay, mental retardation, seizures and mild, non-specific dysmorphic features. Methodology/Principal Findings: We characterized microdeletions at 20q13.33 in six individuals referred for genetic evaluation of developmental delay, mental retardation, and/or congenital anomalies. A comparison to previously reported cases of 20q13.33 microdeletion shows phenotypic overlap, with clinical features that include mental retardation, developmental delay, speech and language deficits, seizures, and behavior problems such as autistic spectrum disorder. There does not appear to be a clinically recognizable constellation of dysmorphic features among individuals with subtelomeric 20q microdeletions. Conclusions/Significance: Based on genotype-phenotype correlation among individuals in this and previous studies, we discuss several possible candidate genes for specific clinical features, including ARFGAP1, CHRNA4 and KCNQ2 and neurodevelopmental deficits. Deletion of this region may play an important role in cognitive development

Host gene expression signatures to identify infection type and organ dysfunction in children evaluated for sepsis: a multicentre cohort study.

BackgroundSepsis is defined as dysregulated host response to infection that leads to life-threatening organ dysfunction. Biomarkers characterising the dysregulated host response in sepsis are lacking. We aimed to develop host gene expression signatures to predict organ dysfunction in children with bacterial or viral infection.MethodsThis cohort study was done in emergency departments and intensive care units of four hospitals in Queensland, Australia, and recruited children aged 1 month to 17 years who, upon admission, underwent a diagnostic test, including blood cultures, for suspected sepsis. Whole-blood RNA sequencing of blood was performed with Illumina NovaSeq (San Diego, CA, USA). Samples with completed phenotyping, monitoring, and RNA extraction by March 31, 2020, were included in the discovery cohort; samples collected or completed thereafter and by Oct 27, 2021, constituted the Rapid Paediatric Infection Diagnosis in Sepsis (RAPIDS) internal validation cohort. An external validation cohort was assembled from RNA sequencing gene expression count data from the observational European Childhood Life-threatening Infectious Disease Study (EUCLIDS), which recruited children with severe infection in nine European countries between 2012 and 2016. Feature selection approaches were applied to derive novel gene signatures for disease class (bacterial vs viral infection) and disease severity (presence vs absence of organ dysfunction 24 h post-sampling). The primary endpoint was the presence of organ dysfunction 24 h after blood sampling in the presence of confirmed bacterial versus viral infection. Gene signature performance is reported as area under the receiver operating characteristic curves (AUCs) and 95% CI.FindingsBetween Sept 25, 2017, and Oct 27, 2021, 907 patients were enrolled. Blood samples from 595 patients were included in the discovery cohort, and samples from 312 children were included in the RAPIDS validation cohort. We derived a ten-gene disease class signature that achieved an AUC of 94·1% (95% CI 90·6-97·7) in distinguishing bacterial from viral infections in the RAPIDS validation cohort. A ten-gene disease severity signature achieved an AUC of 82·2% (95% CI 76·3-88·1) in predicting organ dysfunction within 24 h of sampling in the RAPIDS validation cohort. Used in tandem, the disease class and disease severity signatures predicted organ dysfunction within 24 h of sampling with an AUC of 90·5% (95% CI 83·3-97·6) for patients with predicted bacterial infection and 94·7% (87·8-100·0) for patients with predicted viral infection. In the external EUCLIDS validation dataset (n=362), the disease class and disease severity predicted organ dysfunction at time of sampling with an AUC of 70·1% (95% CI 44·1-96·2) for patients with predicted bacterial infection and 69·6% (53·1-86·0) for patients with predicted viral infection.InterpretationIn children evaluated for sepsis, novel host transcriptomic signatures specific for bacterial and viral infection can identify dysregulated host response leading to organ dysfunction.FundingAustralian Government Medical Research Future Fund Genomic Health Futures Mission, Children's Hospital Foundation Queensland, Brisbane Diamantina Health Partners, Emergency Medicine Foundation, Gold Coast Hospital Foundation, Far North Queensland Foundation, Townsville Hospital and Health Services SERTA Grant, and Australian Infectious Diseases Research Centre

Tris(4-methylpiperazin-1-yl)phosphane, P(NC4H8NMe)(3): Synthesis, structural studies, group 10 and 11 metal complexes and catalytic investigations

Group 10 and 11 metal complexes of a multidentate phosphorus-nitrogen donor ligand tris(4-rnethylpiperazin-1-yl)phosphane, P(NC4H8NMe)(3) (1) are reported. The reactions of 1 with an equimolar amount of CuX (X = Cl, Br and I) afford tetranuclear cubane-like complexes [(CuX){P(NC4H8NMe)(3)}](4) (2, X = Cl; 3, X = Br and 4, X = I) in excellent yield. Treatment of 1 with AuCl(SMe2) produces a mononuclear complex, [(AuCl){P(NC4H8NMe)(3)}] (5). Reaction of 1 with AgCN produces a 2D Ag-I polymeric sheet, [(AgCN)(2){P(NC4H8NMe)(3)}](n) (6) in moderate yield. The similar 1:1 reactions of I with AgX (X = Cl and Br) furnish dinuclear complexes, [(AgX){P(NC4HBNMe)(3)}](2) (7, X = Cl and 8, X = Br). The 2:1 reactions of 1 with [M(COD)Cl-2] (M = Pd or Pt) afford [{P(NC4H8NMe)(3)}(2)MCl2] (9, M = Pd and 10, M = Pt) in quantitative yield. The molecular structures of complexes 1-3 and 6 are established through single-crystal X-ray diffraction studies. The catalytic activity of the Pd-II complex 9 has been investigated in Suzuki cross coupling reactions. ((C) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)

Palladium(II) complex of phosphinic amide, [Pd(Ph2P(O)CH2NPh)(2)], and its catalytic investigation towards Suzuki-Miyaura cross-coupling reactions

The reaction between aniline and chlorodiphenylphosphine in 2:1 molar ratio afforded the aminophosphine C6H5NHPPh2 (1) which on subsequent treatment with paraformaldehyde produced the methylene inserted product, Ph2P(O)CH2NHPh (2). Treatment of (2) with Pd(OAc)(2) in 2:1 molar ratio produced the complex [Pd(Ph2P(O)CH2NPh)(2)] (3), which has been characterized by various spectroscopic and microanalysis data. The catalytic activity of complex (3) has been investigated in Suzuki-Miyaura cross-coupling reactions. The reactions of various aryl halides with phenylboronic acid using (3) as a catalyst gave moderate (35-55%) to high yields (100%) of coupled products

Suzuki cross-coupling reactions catalyzed by palladium complex of an inexpensive phosphinite, 2-diphenylphosphinoxynaphthyl

A facile and less expensive monophosphinite ligand 2-(diphenylphosphinoxy)-naphthyl, C10H7OPPh2(1) has been synthesized from the reaction of β-naphthol with chlorodiphenylphosphine. The mixture of Pd(OAc)2 and 1 catalyzes the Suzuki cross-coupling of a variety of aryl halides with aryl boronic acids at room temperature or at 60°C, giving generally high yields even under low catalytic loads. The effect of solvent, base and catalyst loading on the coupling reaction of aryl halide with arylboronic acid is also described.© Elsevie

Group 11 metal chemistry of a tetradentate ligand, phenylene-1,4-diaminotetra(phosphonite), p-c(6)h(4)[n{p(oc(6)h(4)ome-o)(2)}(2)](2)

The Cu(I), Ag(I), and Au(I) chemistry of a tetradentate ligand (phenylene-1,4-diaminotetra(phosphonite), p-C(6)H(4)[N{P(OC(6)H(4)OMe-o)(2)}(2)](2) (P(2)N Phi NP(2)) (1)) is described. The flexional conformations in 1 leads to interesting structural variations in transition-metal complexes. The reaction of 1 with 4 equiv of CuX (where X = Br and I) produce the tetranuclear complexes, [{Cu(2)(mu-X)(2)(NCCH(3))(2)}(2)(mu-P(2)N Phi NP(2))] (where X = Br (2) or X = 1 (3)) in quantitative yield. Treatment of 3 with an excess of pyridine, 2-(piperazin-1-yl)pydmidine, and pyrazole yielded the tetra-substituted derivatives, [{Cu(2)(mu-I)(2)(L)(2)}(2)(mu-P(2)N Phi NP(2))1 (where L = pyridine (4), 2-(piperazin-1-yl)pydmidine (5), or pyrazole.(6)). Similar reactions of 3 with 1,10-phenanthroline (phen) and 2,2'-bipyridine in a 1:2 molar ratio afford the disubstituted derivatives, [(Cu(2)(mu-I))(2)l(2)(phen)(2)(mu-P(2)N Phi NP(2))] (7) and [(Cu(2)(mu-I))(2)l(2)(bipy)(2)(mu-P(2)N Phi NP(2))] (8). The o-methoxyphenoxy substituents on phosphorus in complexes 5 and 7 adopt approximately parallel planar conformations and contain lattice solvents. The reaction of 3 with 1,4-diazabicyclo[2.2.2]octane (DABCO) in a 1:2 molar ratio in a dichloromethane-acetonitrile mixture leads to the formation of an ionic complex [N(CH(2)CH(2))(3)N(+)CH(2)Cl](2)[(Cu(2)(Cl)(I)(2))(2)(NCCH(3))(2)(mu-p2N Phi NP(2))](2-) (9), as a result of the chloromethylation of DABCO. Treatment of 1 with 4 equiv of AgClO(4) produces [{Ag(2)(mu-ClO(4))(2))(2)(C(4)H(8)O)(2)}(2)(mu-P(2)N Phi NP(2))] (10). Displacement of perchlorate ions in 10 by PhN{P(OC(6)H(4)OMe-o)(2)}(2) (PNP) or 2,2'-bipyridine yielded [(mu-PNP)(2)Ag(2)(mu-P(2)N Phi NP(2))Ag(2)- (mu-PNP)(2)](ClO(4))(4) (11) and [{Ah(2)(bipy)(2)}(2)(mu-P(2)N Phi NP(2))](ClO(4))(4) (12), respectively. The similar reaction of 1 with 2 equiv of AgOTf, in the presence of 4,4'-bipyridine, gave a three-dimensional Ag(I) coordination polymer, [{Ag(2)(C(10)H(8)N(2))(2) (CH(3)CN)(2)}(2)(P(2)N Phi NP(2))](n)(OTf)(4n) (13). The reactions of 1 with [AuCl(SMe(2))], in appropriate ratios, afford the tetranuclear and dinuclear complexes, [(Au(2)Cl(2))(2)(mu-P(2)N Phi NP(2))] (14) and [(AuCl)(2)(P(2)N Phi NP(2))] (15). Complex 14 undergoes moisture-assisted P-N bond cleavage in the presence of PhN{P(OC(6)H(4)OMe-o)(2))(2) to give [p-NH(2)C(6)H(4)N(P(OC(6)H(4)OMe-o)(2)}(2)Au(2)Cl(2)] (17) and [PhN{P(OC(6)H(4)OMe-o)(2)}(2)Au(2)Cl(2)]. The strucures of the complexes 5, 7-10, 12-15, and 17 are confirmed by single-crystal X-ray diffraction studies