Studii preliminare privind cultura plantelor legumicole în ghivece şi containere

This paper presents a literature review of the vegetable growing in pots and containers. Growing vegetables in this system it is known for a long time in the countries of Western Europe and in some areas of our country. Adopting this system in Romania requires in-depth studies regarding: the suitable species, the type of pots and soil recipes needed, crop establishment and maintenance (fertilizers aplication, irrigation) and, in some cases, optimization of the certain referring to light and placement of pots and containers. This cultivation system is spread mainly in peri-urban areas were the interest among gardeners to grow their own crops and secure their vegetable needs is high and has a favorable environment. Vegetable plants cultivated in pots and containers present a large diversity worldwide but, within this diversity, the climatic conditions from our country must be appropriate for an efficient activity

Rezultate partiale privind tipurile de substraturi folosite pentru obţinerea culturilor legumicole de tip microgreens

The paper addresses research on the use of four types of substrates (coconut substrate, 80% peat + 20% coconut waste, 80% peat + 20% sand and80% peat + 20% pearlite) to obtain the products microgreens type vegetables, in order to establish the best substrate variant. The best results were obtained on the coconut waste substrate

Studii preliminare privind alegerea şi pregătirea substraturilor pentru cultura la ghivece şi container

The paper presents a study on the main pedological, agrochemical and biological indices of the different types of substrate used for growing vegetables in pots and containers in order to optimize the growth and development of plants. The researchewere carried out in an experimental field set up at the Didactic Center of the University of Agricultural Sciences and Veterinary Medicine Iasi. Three types of substrate were used: S1 -substrate made from 35% garden soil, 35% compost, 20% peat, 10% sandand pearlite, S2-substrate made of earth 35% garden, 20% compost, 35% peat, 10% sand and pearlite; S3 -commercially available substrate. Determinations havebeen made on:nutrients, pH, humidity and granulometry. Substrate quality assessment was performed on the basis of yield and harvest qualit

Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture

BACKGROUND: Stromal fibroblasts associated with in situ and invasive breast carcinoma differ phenotypically from fibroblasts associated with normal breast epithelium, and these alterations in carcinoma-associated fibroblasts (CAF) may promote breast carcinogenesis and cancer progression. A better understanding of the changes that occur in fibroblasts during carcinogenesis and their influence on epithelial cell growth and behavior could lead to novel strategies for the prevention and treatment of breast cancer. To this end, the effect of CAF and normal breast-associated fibroblasts (NAF) on the growth of epithelial cells representative of pre-neoplastic breast disease was assessed. METHODS: NAF and CAF were grown with the nontumorigenic MCF10A epithelial cells and their more transformed, tumorigenic derivative, MCF10AT cells, in direct three-dimensional co-cultures on basement membrane material. The proliferation and apoptosis of MCF10A cells and MCF10AT cells were assessed by 5-bromo-2'-deoxyuridine labeling and TUNEL assay, respectively. Additionally, NAF and CAF were compared for expression of insulin-like growth factor II as a potential mediator of their effects on epithelial cell growth, by ELISA and by quantitative, real-time PCR. RESULTS: In relatively low numbers, both NAF and CAF suppressed proliferation of MCF10A cells. However, only NAF and not CAF significantly inhibited proliferation of the more transformed MCF10AT cells. The degree of growth inhibition varied among NAF or CAF from different individuals. In greater numbers, NAF and CAF have less inhibitory effect on epithelial cell growth. The rate of epithelial cell apoptosis was not affected by NAF or CAF. Mean insulin-like growth factor II levels were not significantly different in NAF versus CAF and did not correlate with the fibroblast effect on epithelial cell proliferation. CONCLUSION: Both NAF and CAF have the ability to inhibit the growth of pre-cancerous breast epithelial cells. NAF have greater inhibitory capacity than CAF, suggesting that the ability of fibroblasts to inhibit epithelial cell proliferation is lost during breast carcinogenesis. Furthermore, as the degree of transformation of the epithelial cells increased they became resistant to the growth-inhibitory effects of CAF. Insulin-like growth factor II could not be implicated as a contributor to this differential effect of NAF and CAF on epithelial cell growth

Estudio de fortalecimiento de prestadores de Servicios en las Cuencas Lecheras del Norte y Centro Sur de Nicaragua

El Objetivo General del estudio es identificar, caracterizar, clasificar y analizar la demanda y la oferta de servicios de apoyo a las empresas de los diferentes eslabones de las cadenas de lácteos, en los municipios de Camoapa, Matiguás y Santo Tomás para establecer las brechas existentes y trazar los lineamientos necesarios para el diseño de un programa de fortalecimiento y desarrollo de capacidades de los prestadores de Servicios de Desarrollo Empresarial (SDE). Estos municipios se consideraron representativos de los Departamentos de Boaco, Matagalpa y Chontales y los municipios ganaderos del Norte de la RAAS y del Sur de la RAAN, los que tienen más del 60 % de la población de ganado bovino de Nicaragua, y una producción conjunta de leche de 179.2 millones de galones (2005), o sea el 62 % del total producido en el país y 380,330 quintales de queso equivalente al 87 % de la producción total del país

Cap-Gly Proteins at Microtubule Plus Ends: Is EB1 Detyrosination Involved?

Localization of CAP-Gly proteins such as CLIP170 at microtubule+ends results from their dual interaction with α-tubulin and EB1 through their C-terminal amino acids −EEY. Detyrosination (cleavage of the terminal tyrosine) of α-tubulin by tubulin-carboxypeptidase abolishes CLIP170 binding. Can detyrosination affect EB1 and thus regulate the presence of CLIP170 at microtubule+ends as well? We developed specific antibodies to discriminate tyrosinated vs detyrosinated forms of EB1 and detected only tyrosinated EB1 in fibroblasts, astrocytes, and total brain tissue. Over-expressed EB1 was not detyrosinated in cells and chimeric EB1 with the eight C-terminal amino acids of α-tubulin was only barely detyrosinated. Our results indicate that detyrosination regulates CLIPs interaction with α-tubulin, but not with EB1. They highlight the specificity of carboxypeptidase toward tubulin