Effect of adjuvants and nitrogen sources on in vitro shoot regeneration and clonal propagation of medicinally important plant Eryngium foetidum L

In vitro regeneration of medicinally important plant Eryngium foetidum L. was established by developing a protocol for successful faster clonal propogation. Young leaves of the plant were taken and cultured on 1x MS medium supplemented with different concentrations and combinations of 6- Benzyl amino purine (BAP), 1-Napthylacetic acid (NAA) and Kinetin (Kin). Leaves cultured on 2mg/L BAP and 1mg/L NAA showed better response in short duration of time when compared to other concentrations and combinations. Induced shoots obtained from standardized media were used as explant and cultured on 1x MS medium containing 2mg/L BAP and 1mg/L NAA fortified with 10% of different nitrogen sources like  tryptone, peptone, beef extract, yeast extract and adjuvants like coconut milk, tomato juice and banana pulp to observe the growth response. It was observed that media containing 10% coconut milk and 10% peptone showed better result when compared to other adjuvants and nitrogen sources. The well developed plantlets were hardened and successfully transferred to the field with 85% survival rate

Phytochemical analysis and free radical scavenging potential of Baliospermum montanum (Willd.) Muell. leaf

Baliospermum montanum (Willd.) Muell. is used in India for reducing oxidative stress. The main objective of the study was to investigate phytochemical and antioxidant activities to justify the use of this plant in medicines. Antioxidant activity of different concentrations of methanolic leaf extract was evaluated with the determination of total phenolic, DPPH* radical scavenging assay, and ABTS+ decolouration assay. The total phenolic content was higher in this extract. The antioxidant potential of the extract was well established with DPPH*, which provide a basis for the traditional use of this plant in medicines

Antimicrobial Natural Cellulose Fibers from Hyptis suaveolens for Potential Biomedical and Textiles Applications

In this research, tulsi (wild type) stalks were treated with NaOH to obtain natural cellulosic fibers. X-ray diffractions showed typical cellulose diffraction peak at 22.7°. The fibers had a tensile strength of 445 MPa, higher than fibers obtained from other agricultural residues. The fibers also displayed good thermal stability up to 300℃ making it suitable for a wide range of applications. The fibers inherited the antimicrobial properties from the stalks and showed high activities against both Gram positive and Gram negative bacteria. The extracted fibers could be used in composites and manufacturing tents and canvas