Study on the Activity of PI3K/AKT, Death Receptor and 14-3-3 Mediated Signaling Pathways Regulating Hepatocyte Apoptosis during Rat Liver Regeneration

Studies have shown that apoptosis is closely related to the rat liver regeneration. To understand the mechanism of hepatocyte apoptosis during rat liver regeneration at the gene transcription level, the Rat Genome 230 2.0 Array was used to determine the expression changes of genes. Then the genes associated with cell apoptosis were searched by GO and NCBI databases, and cell apoptosis signaling pathways were searched by the database of QIAGEN and KEGG. Their signaling activities were calculated by spectral function E(t). The mechanism of hepatocyte apoptosis during rat liver regeneration was analyzed by Ingenuity Pathway Analysis 9.0 (IPA). The results showed that among the 27 signaling pathways regulating cell apoptosis, the E(t) values of Apoptosis signaling pathway and 14-3-3 mediated signaling pathway were significantly increased in the progression phase (6-72h after PH) of rat liver regeneration, and the E(t) values of hepatocyte apoptosis mediated by mitochondria rout were also significantly increased. The E(t) values of death receptor signaling pathway and PI3K/AKT branch of 14-3-3 mediated signaling pathway were significantly increased in the progression phase and the terminal phase (72-168h after PH) of rat liver regeneration, and the E(t) values of hepatocyte apoptosis mediated by cytomembrane route and nucleus route were also significantly increased. Conclusion: PI3K/AKT, death receptor and mitochondria branch played a key role in promoting cell apoptosis during rat liver regeneration

Large probe arrays for measuring mean and time dependent local oil volume fraction and local oil velocity component distributions in inclined oil-in-water flows

Arrays of dual-sensor and four-sensor needle conductance probes have been used to measure the mean and time dependent local properties of upward inclined, bubbly oil-in-water flows (also known as dispersed oil-in-water flows) in a 153mm diameter pipe. The flow properties that were measured were (i) the local in-situ oil volume fraction ; (ii) the local oil velocity in the axial direction of the pipe (the direction); and (iii) the local oil velocity in the direction from the lower side of the inclined pipe to its upper side (the direction). Oil velocities in the direction (orthogonal to the and directions) were found to be negligible. For all of the flow conditions investigated it was found that the mean value of varied from a maximum value at the upper side of the inclined pipe to a minimum value at the lower side, and that the rate of decrease of this mean value of with distance in the direction became greater as the pipe inclination angle from the vertical was increased. It was also found that the mean value of was greatest at the upper side of the inclined pipe and decreased towards the lower side of the inclined pipe, the rate of decrease with distance in the direction again becoming greater as was increased. For , a water volumetric flow rate , an oil volumetric flow rate and using a sampling period over a total time interval of , it was found that at the upper side of the inclined pipe the standard deviation in was 31.6% of the mean value of . Furthermore for , , and it was found that the standard deviation in the cross-pipe oil velocity component was approximately equal to the standard deviation in the axial velocity component . These large temporal variations in the local flow properties have been attributed to the presence of large scale Kelvin-Helmholtz waves which intermittently appear in the flow. It is believed that the techniques outlined in this paper for measuring the standard deviation of local flow properties as a function of the sampling period will be of considerable value in validating mathematical models of time dependent oil-water flows. It should be noted that the principal focus of this paper is on the measurement techniques that were used and the methods of data analysis rather than the presentation of exhaustive experimental results at numerous different flow conditions

Negative control of CSL gene transcription by stress/DNA damage response and p53.

CSL is a key transcriptional repressor and mediator of Notch signaling. Despite wide interest in CSL, mechanisms responsible for its own regulation are little studied. CSL down-modulation in human dermal fibroblasts (HDFs) leads to conversion into cancer associated fibroblasts (CAF), promoting keratinocyte tumors. We show here that CSL transcript levels differ among HDF strains from different individuals, with negative correlation with genes involved in DNA damage/repair. CSL expression is negatively regulated by stress/DNA damage caused by UVA, Reactive Oxygen Species (ROS), smoke extract, and doxorubicin treatment. P53, a key effector of the DNA damage response, negatively controls CSL gene transcription, through suppression of CSL promoter activity and, indirectly, by increased p21 expression. CSL was previously shown to bind p53 suppressing its activity. The present findings indicate that p53, in turn, decreases CSL expression, which can serve to enhance p53 activity in acute DNA damage response of cells

Detailed quantitative comparison of half-bridge modular multilevel converter modelling methods

This paper presents a detailed comparison of different modelling methods of the half-bridge modular multilevel converter (HB-MMC), namely, switching function, Thevenin equivalent and averaged, considering both MMC implementations (large and reduced number of cells). The theoretical basis that underpins each modelling method are discussed. Offline PSCAD simulations are used to validate user-defined switching function and averaged MMC models against the Thevenin equivalent model provided in PSCAD library for accuracy, considering steady-state and dc fault conditions. Furthermore, the RTDS based real-time simulation results of the user-defined HB-MMC switching function model are validated against the above mentioned offline models, considering steady-state and dc short circuit fault operations. Simulation speed and efficiency of different offline HB-MMC models being studied in this paper are compared. From comprehensive corroboration of different HB-MMC models presented in this paper, it has been found that the averaged, switching function and Thevenin equivalent models produce practically identical results during steady-state and dc faults. In detailed offline and real-time simulation studies where fundamental and harmonic dynamics are of interest, switching function model is found to be faster and computational efficient compared to the Thevenin equivalent model

Fast Mesh-Based Medical Image Registration

In this paper a fast triangular mesh based registration method is proposed. Having Template and Reference images as inputs, the template image is triangulated using a content adaptive mesh generation algorithm. Considering the pixel values at mesh nodes, interpolated using spline interpolation method for both of the images, the energy functional needed for image registration is minimized. The minimization process was achieved using a mesh based discretization of the distance measure and regularization term which resulted in a sparse system of linear equations, which due to the smaller size in comparison to the pixel-wise registration method, can be solved directly. Mean Squared Difference (MSD) is used as a metric for evaluating the results. Using the mesh based technique, higher speed was achieved compared to pixel-based curvature registration technique with fast DCT solver. The implementation was done in MATLAB without any specific optimization. Higher speeds can be achieved using C/C++ implementations.Comment: Accepted manuscript for ISVC'201

Theory of Chiral Modulations and Fluctuations in Smectic-A Liquid Crystals Under an Electric Field

Chiral liquid crystals often exhibit periodic modulations in the molecular director; in particular, thin films of the smectic-C* phase show a chiral striped texture. Here, we investigate whether similar chiral modulations can occur in the induced molecular tilt of the smectic-A phase under an applied electric field. Using both continuum elastic theory and lattice simulations, we find that the state of uniform induced tilt can become unstable when the system approaches the smectic-A--smectic-C* transition, or when a high electric field is applied. Beyond that instability point, the system develops chiral stripes in the tilt, which induce corresponding ripples in the smectic layers. The modulation persists up to an upper critical electric field and then disappears. Furthermore, even in the uniform state, the system shows chiral fluctuations, including both incipient chiral stripes and localized chiral vortices. We compare these predictions with observed chiral modulations and fluctuations in smectic-A liquid crystals.Comment: 11 pages, including 9 postscript figures, uses REVTeX 3.0 and epsf.st

Study on the Activity of PI3K/AKT, Death Receptor and 14-3-3 Mediated Signaling Pathways Regulating Hepatocyte Apoptosis during Rat Liver Regeneration

Studies have shown that apoptosis is closely related to the rat liver regeneration. To understand the mechanism of hepatocyte apoptosis during rat liver regeneration at the gene transcription level, the Rat Genome 230 2.0 Array was used to determine the expression changes of genes. Then the genes associated with cell apoptosis were searched by GO and NCBI databases, and cell apoptosis signaling pathways were searched by the database of QIAGEN and KEGG. Their signaling activities were calculated by spectral function E(t). The mechanism of hepatocyte apoptosis during rat liver regeneration was analyzed by Ingenuity Pathway Analysis 9.0 (IPA). The results showed that among the 27 signaling pathways regulating cell apoptosis, the E(t) values of Apoptosis signaling pathway and 14-3-3 mediated signaling pathway were significantly increased in the progression phase (6-72h after PH) of rat liver regeneration, and the E(t) values of hepatocyte apoptosis mediated by mitochondria rout were also significantly increased. The E(t) values of death receptor signaling pathway and PI3K/AKT branch of 14-3-3 mediated signaling pathway were significantly increased in the progression phase and the terminal phase (72-168h after PH) of rat liver regeneration, and the E(t) values of hepatocyte apoptosis mediated by cytomembrane route and nucleus route were also significantly increased. Conclusion: PI3K/AKT, death receptor and mitochondria branch played a key role in promoting cell apoptosis during rat liver regeneration

HSD17B7 gene in self-renewal and oncogenicity of keratinocytes from Black versus White populations.

Human populations of Black African ancestry have a relatively high risk of aggressive cancer types, including keratinocyte-derived squamous cell carcinomas (SCCs). We show that primary keratinocytes (HKCs) from Black African (Black) versus White Caucasian (White) individuals have on average higher oncogenic and self-renewal potential, which are inversely related to mitochondrial electron transfer chain activity and ATP and ROS production. HSD17B7 is the top-ranked differentially expressed gene in HKCs and Head/Neck SCCs from individuals of Black African versus Caucasian ancestries, with several ancestry-specific eQTLs linked to its expression. Mirroring the differences between Black and White HKCs, modulation of the gene, coding for an enzyme involved in sex steroid and cholesterol biosynthesis, determines HKC and SCC cell proliferation and oncogenicity as well as mitochondrial OXPHOS activity. Overall, the findings point to a targetable determinant of cancer susceptibility among different human populations, amenable to prevention and management of the disease

The Arabidopsis anaphase-promoting complex/cyclosome subunit 8 is required for male meiosis

Faithful chromosome segregation is required for both mitotic and meiotic cell divisions and is regulated by multiple mechanisms including the anaphase-promoting complex/cyclosome (APC/C), which is the largest known E3 ubiquitin-ligase complex and has been implicated in regulating chromosome segregation in both mitosis and meiosis in animals. However, the role of the APC/C during plant meiosis remains largely unknown. Here, we show that Arabidopsis APC8 is required for male meiosis. We used a combination of genetic analyses, cytology and immunolocalisation to define the function of AtAPC8 in male meiosis. Meiocytes from apc8-1 plants exhibit several meiotic defects including improper alignment of bivalents at metaphase I, unequal chromosome segregation during anaphase II, and subsequent formation of polyads. Immunolocalisation using an antitubulin antibody showed that APC8 is required for normal spindle morphology. We also observed mitotic defects in apc8-1, including abnormal sister chromatid segregation and microtubule morphology. Our results demonstrate that Arabidopsis APC/C is required for meiotic chromosome segregation and that APC/C-mediated regulation of meiotic chromosome segregation is a conserved mechanism among eukaryotes

New Lump-like Structures in Scalar-field Models

In this work we investigate lump-like solutions in models described by a single real scalar field. We start considering non-topological solutions with the usual lump-like form, and then we study other models, where the bell-shape profile may have varying amplitude and width, or develop a flat plateau at its top, or even induce a lump on top of another lump. We suggest possible applications where these exotic solutions might be used in several distinct branches of physics.Comment: REvTex4, twocolumn, 10 pages, 9 figures; new reference added, to appear in EPJ