FGF/heparin differentially regulates Schwann cell and olfactory ensheathing cell interactions with astrocytes: a role in astrocytosis

After injury, the CNS undergoes an astrocyte stress response characterized by reactive astrocytosis/proliferation, boundary formation, and increased glial fibrillary acidic protein (GFAP) and chondroitin sulfate proteoglycan (CSPG) expression. Previously, we showed that in vitro astrocytes exhibit this stress response when in contact with Schwann cells but not olfactory ensheathing cells (OECs). In this study, we confirm this finding in vivo by demonstrating that astrocytes mingle with OECs but not Schwann cells after injection into normal spinal cord. We show that Schwann cell-conditioned media (SCM) induces proliferation in monocultures of astrocytes and increases CSPG expression in a fibroblast growth factor receptor 1 (FGFR1)-independent manner. However, SCM added to OEC/astrocyte cocultures induces reactive astrocytosis and boundary formation, which, although sensitive to FGFR1 inhibition, was not induced by FGF2 alone. Addition of heparin to OEC/astrocyte cultures induces boundary formation, whereas heparinase or chlorate treatment of Schwann cell/astrocyte cultures reduces it, suggesting that heparan sulfate proteoglycans (HSPGs) are modulating this activity. In vivo, FGF2 and FGFR1 immunoreactivity was increased over grafted OECs and Schwann cells compared with the surrounding tissue, and HSPG immunoreactivity is increased over reactive astrocytes bordering the Schwann cell graft. These data suggest that components of the astrocyte stress response, including boundary formation, astrocyte hypertrophy, and GFAP expression, are mediated by an FGF family member, whereas proliferation and CSPG expression are not. Furthermore, after cell transplantation, HSPGs may be important for mediating the stress response in astrocytes via FGF2. Identification of factors secreted by Schwann cells that induce this negative response in astrocytes would further our ability to manipulate the inhibitory environment induced after injury to promote regeneration

In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.

Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the biocompatibility studies indicated that the nanofibers exhibited a high degree of cellular biocompatibility and short-term intraocular tolerance, indicating that they might be applied as drug carrier for ophthalmic use

Perception of Culture in a way of influencing in Fashion of Dress: Analysis of fashion of dresses in Kandyan Kingdom of Sri Lanka

Perception is a scientific process by which people got to know and get ideas about environment. Several modalities of perception are there like Vision, hearing, tactile sensation, taste and vibration sensation. People influence in certain fashion through this process. Important factors involve in perception in a society are culture (norms and values), level of communication, accepted colours. For example same visual stimuli like elephant was percept in Sinhalese culture as a dignity but westerners’ percept it as mere big animal not the dignity. This means same stimuli percept in different cultures in different ways

Indian Influences in King’s Costumes of Ancient Sri Lankan Kandyan Era

Sri Lanka is centrally located in the Indian Ocean below the southern tip of the Indian peninsula; being separated by the Palk Straits which is only 35Km long, Thus Sinhalese had continuous Indian influences since time immemorial. According to the great chronicle Mahavamsa the first human settlement of Ceylon begins with the arrival of Vijaya and his 700 companions who had been banished for misconduct from the Kingdom of ‘Sihapura’ in Northern India . According to the Mahavamsa the entry of Buddhism to Sri Lanka occurred in the region of Devanampiya Tissa (250-210 BC) with the patronage of the great Mauran Emperor Asoka

PENGEMBANGAN APLIKASI KAMUS BAHASA TETUM BERBASIS WEB

Bahasa Tetum merupakan anak cabang dari bahasa Autronesia, degan penutur utama diwilayah timor. Di Timor Leste bahasa ini merupakan bahasa resmi, selain bahasa Portugis. Dibawah konstitusi Negara, Bahasa Indonesia dan Bahasa Inggris merupakan bahasa sehari-hari. Bagi penduduk bahasa Tetum merupakan bahasa pemersatu antar suku seperti layaknya bahasa Indonesia. Dalam perancangan sistem ini diperlukan beberapa software yang di antaranya appServer yang didalamnya sudah termuat PHP,database MySQL,Web Server Apache, dan PHPMyAdmin. Diharapkan juga kamus online ini bisa menjadi sarana belajar yang efisien bagi siapa saja yang ingin mempelajari bahasa Tetum, bahasa Indonesia dan bahasa Inggris. Kata kunci : Kamus online, Tetum, Indonesia, Inggris

Inclusion of shrimp waste meal in diet of free-range chickens

Shrimp waste meal (SWM) is a by-product from the processing of shrimp for human consumption. The value of SMW in feeding poultry is not well documented. The objective of this study was to determine the energy value and optimal inclusion level of SWM in the diet of growing chickens. A total of 180 one-day-old broilers were randomly assigned to five treatments with 0, 50, 100, 150 and 200 g/kg of SWM included in their diet. There were six replicates of six birds for each treatment. Dry matter intake (DMI) was not affected by the level of SWM that was fed. Retained dry matter varied from 72.39% in the diet that did not contain SWM to 66.97% in the diet with 200 g/kg of SWM. Nitrogen retention (NR) ranged from 54.70% to 70.10%; N ingested was between 18.71% and 24.03%. Energy intake ranged from 73.57% to 69.33% for the control and the diet with 200 g/kg of SWM, respectively. NR improved with increasing SWM inclusion levels. The apparent metabolizable energy (AME) and corrected apparent energy metabolizable (AMEn) ranged from 2928 to 2527 kcal/kg and 2774 to 2329 kcal/kg, respectively, relative to the control and 200 g/kg SWM diets. The energy consumption, in kcal/kg, of SWM consumed was AME = 2332-6.971 x SWM and AMEn = 2113-8.128 x SWM. High levels of SWM reduce the dry matter metabolization coefficient and metabolizable energy values in broilers during the growing phase, so it is recommended that up to 100 g/kg should be included, which would provide an AMEn of 1300.2 kcal/kg for free-range chickens in dry matter

Behaviour and performance of lactating sows housed in different types of farrowing rooms during summer

AbstractThirty mixed-parity Landrace×Large White sows were used to evaluate the effects of the type of farrowing room on 28-day lactation behaviour under tropical conditions during summer. The sows were allocated in a completely randomised design with three treatments with 10 replicates according to parity number and body weight, with each animal being considered an experimental unit. The treatments consisted of a conventional farrowing room (T1); a conventional farrowing room with floor cooling under the sow (T2); and a semi-outdoor farrowing room without a cage and with access to a fenced field (T3). The sows from T1 and T2 groups were exposed to mean maximum and minimum environmental temperatures of 25.7 and 21.0°C, respectively, and the sows from the T3 group to average maximum and minimum environmental temperatures of 26.5 and 20.7°C, respectively. The feed consumption of T3 sows was numerically higher than the T1 and T2 sows (+9.5% on average). The body-weight loss was influenced at 28days (P<0.10) by treatment, being that the T3 sows gained weight (+4.7kg) while the T1 and T2 sows lost weight (−11.9 and −3.7kg, respectively for T1 and T2). The T3 sows showed a higher percentual litter mortality than the T1 and T2 sows (3.2% vs. 0% vs. 7.8%, respectively for T1, T2 and T3 sows). From farrowing until day 28 of lactation, the T2 and T3 sows showed higher lactation efficiency when compared with the T1 sows (72% vs. 87% vs. 88%, respectively for T1, T2 and T3 sows). The T1 sows showed higher (P<0.01) frequencies of visits to the feeder and drinker (+38% on average). The T3 sows spent more time (P<0.01) at the drinker than T1 and T2 sows (23 vs. 23 vs. 32min, respectively for T1, T2 and T3 sows). The T3 sows showed a higher (P<0.10) frequency of nursing than the other treatments (+15% on average). T1 and T2 sows were found to spend more time (P<0.01) performing other postures during 24h than sows maintained in T3 (50 vs. 51 vs. 22min/d, respectively for T1, T2 and T3). It is concluded that cooling of the floor under the sow in the conventional farrowing room or the use of semi-outdoor farrowing rooms improves the thermal environment and the lactation efficiency of the sows housed in hot ambient temperatures at 28-day lactation in the summer period, indicating an improved welfare

Template coexistence in prebiotic vesicle models

The coexistence of distinct templates is a common feature of the diverse proposals advanced to resolve the information crisis of prebiotic evolution. However, achieving robust template coexistence turned out to be such a difficult demand that only a class of models, the so-called package models, seems to have met it so far. Here we apply Wright's Island formulation of group selection to study the conditions for the coexistence of two distinct template types confined in packages (vesicles) of finite capacity. In particular, we show how selection acting at the level of the vesicles can neutralize the pressures towards the fixation of any one of the template types (random drift) and of the type with higher replication rate (deterministic competition). We give emphasis to the role of the distinct generation times of templates and vesicles as yet another obstacle to coexistence.Comment: 7 pages, 8 figure