Gas chromatography-mass spectrometry-based metabolite profiling of Salmonella enterica serovar Typhimurium differentiates between biofilm and planktonic phenotypes

The aim of this study was to utilize gas chromatography coupled with mass spectrometry (GC-MS) to compare and identify patterns of biochemical change between Salmonella cells grown in planktonic and biofilm phases and Salmonella biofilms of different ages. Our results showed a clear separation between planktonic and biofilm modes of growth. The majority of metabolites contributing to variance between planktonic and biofilm supernatants were identified as amino acids, including alanine, glutamic acid, glycine, and ornithine. Metabolites contributing to variance in intracellular profiles were identified as succinic acid, putrescine, pyroglutamic acid, and N-acetylglutamic acid. Principal-component analysis revealed no significant differences between the various ages of intracellular profiles, which would otherwise allow differentiation of biofilm cells on the basis of age. A shifting pattern across the score plot was illustrated when analyzing extracellular metabolites sampled from different days of biofilm growth, and amino acids were again identified as the metabolites contributing most to variance. An understanding of biofilm-specific metabolic responses to perturbations, especially antibiotics, can lead to the identification of novel drug targets and potential therapies for combating biofilm-associated diseases. We concluded that under the conditions of this study, GC-MS can be successfully applied as a high-throughput technique for "bottom-up" metabolomic biofilm research

Estimates of hypolimnetic oxygen deficits in ponds

Shallow tropical integrated culture ponds in the Pearl River Delta, China, have been found to stratify almost daily, with high organic loadings and dense algal growth. The dissolved oxygen (DO) concentration is super-saturated in the epilimnion and is under 2 mg/l in the hypolimnion (>1m). The compensation depth corresponds to twice the Secchi disk depth ranging from 50 to 80cm. As a result, little or no net oxygen is produced in the hypolimnion (>1m). The low DO concentration in the hypolimnion causes organic materials, such as unused organic wastes and senescent algae cells, to be incompletely oxidized, since the rate of oxygen consumption by oxidable matter in water is dependent on the dissolved oxygen concentration in water. This material becomes the source of hypolimnetic oxygen deficits (HOD) which can drive whole pond DO to a dangerously low level, should sudden destratification occur. An improved estimate of hypolimnetic oxygen deficits is introduced in this article, and the advantages of this method are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72126/1/j.1365-2109.1989.tb00341.x.pd

Longitudinal broadening of near side jets due to parton cascade

Longitudinal broadening along $\Delta\eta$ direction on near side in two-dimensional ($\Delta\phi \times \Delta\eta$) di-hadron correlation distribution has been studied for central Au+Au collisions at $\sqrt{s_{NN}}$ = 200 GeV, within a dynamical multi-phase transport model. It was found that the longitudinal broadening is generated by a longitudinal flow induced by strong parton cascade in central Au+Au collisions, in comparison with p+p collisions at $\sqrt{s_{NN}}$ = 200 GeV. The longitudinal broadening may shed light on the information about strongly interacting partonic matter at RHIC.Comment: 5 pages, 4 figures; accepted by Eur. Phys. J.

Thermal and ground-state entanglement in Heisenberg XX qubit rings

We study the entanglement of thermal and ground states in Heisernberg $XX$ qubit rings with a magnetic field. A general result is found that for even-number rings pairwise entanglement between nearest-neighbor qubits is independent on both the sign of exchange interaction constants and the sign of magnetic fields. As an example we study the entanglement in the four-qubit model and find that the ground state of this model without magnetic fields is shown to be a four-body maximally entangled state measured by the $N$-tangle.Comment: Four pages and one figure, small change

Neutrinos in Non-linear Structure Formation - The Effect on Halo Properties

We use N-body simulations to find the effect of neutrino masses on halo properties, and investigate how the density profiles of both the neutrino and the dark matter components change as a function of the neutrino mass. We compare our neutrino density profiles with results from the N-one-body method and find good agreement. We also show and explain why the Tremaine-Gunn bound for the neutrinos is not saturated. Finally we study how the halo mass function changes as a function of the neutrino mass and compare our results with the Sheth-Tormen semi-analytic formulae. Our results are important for surveys which aim at probing cosmological parameters using clusters, as well as future experiments aiming at measuring the cosmic neutrino background directly.Comment: 20 pages, 8 figure

Measurement of Cosmic-ray Muon-induced Spallation Neutrons in the Aberdeen Tunnel Underground Laboratory

AbstractMuon-induced neutrons are one of the major backgrounds to various underground experiments, such as dark matter searches, low-energy neutrino oscillation experiments and neutrino-less double beta-decay experiments. Previous experiments on the underground production rate of muon-induced neutrons were mostly carried out either at shallow sites or at very deep sites. The Aberdeen Tunnel experiment aims to measure the neutron production rate at a moderate depth of 611 meters water equivalent. Our apparatus comprises of six layers of plastic-scintillator hodoscopes for tracking the incident cosmic-ray muons, and 760 L of gadolinium-doped liquid-scintillator for both neutron production and detection targets. In this paper, we describe the design and the performance of the apparatus. The preliminary result on the measurement of neutron production rate is also presented

Configurations of mother-child and father-child attachment as predictors of internalizing and externalizing behavioral problems: An individual participant data (IPD) meta-analysis

An unsettled question in attachment theory and research is the extent to which children's attachment patterns with mothers and fathers jointly predict developmental outcomes. In this study, we used individual participant data (IPD) meta-analysis to assess whether early attachment networks with mothers and fathers are associated with children's internalizing and externalizing behavioral problems. Following a pre-registered protocol, data from 9 studies and 1,097 children (mean age: 28.67 months) with attachment classifications to both mothers and fathers were included in analyses. We used a linear mixed effects analysis to assess differences in children's internalizing and externalizing behavioral problems as assessed via the average of both maternal and paternal reports based on whether children had two, one, or no insecure (or disorganized) attachments. Results indicated that children with an insecure attachment relationship with one or both parents were at higher risk for elevated internalizing behavioral problems compared with children who were securely attached to both parents. Children whose attachment relationships with both parents were classified as disorganized had more externalizing behavioral problems compared to children with either one or no disorganized attachment relationship with their parents. Across attachment classification networks and behavioral problems, findings suggest (a) an increased vulnerability to behavioral problems when children have insecure or disorganized attachment to both parents, and (b) that mother-child and father-child attachment relationships may not differ in the roles they play in children's development of internalizing and externalizing behavioral problems

Microbial reduction and precipitation of vanadium (V) in groundwater by immobilized mixed anaerobic culture

Vanadium is an important contaminant impacted by natural and industrial activities. Vanadium (V) reduction efficiency as high as 87.0% was achieved by employing immobilized mixed anaerobic sludge as inoculated seed within 12 h operation, while V(IV) was the main reduction product which precipitated instantly. Increasing initial V(V) concentration resulted in the decrease of V(V) removal efficiency, while this index increased first and then decreased with the increase of initial COD concentration, pH and conductivity. High-throughput 16S rRNA gene pyrosequencing analysis indicated the decreased microbial diversity. V(V) reduction was realized through dissimilatory reduction process by significantly enhanced Lactococcus and Enterobacter with oxidation of lactic and acetic acids from fermentative microorganisms such as the enriched Paludibacter and the newly appeared Acetobacterium, Oscillibacter. This study is helpful to detect new functional species for V(V) reduction and constitutes a step ahead in developing in situ bioremediations of vanadium contamination. (C) 2015 Elsevier Ltd. All rights reserved.National Natural Science Foundation of China (NSFC) [41440025, 21307117]; Beijing Excellent Talent Training Project [2013D009015000003]; Beijing Higher Education Young Elite Teacher Project [YETP0657]SCI(E)[email protected]

Pulsar-wind nebulae and magnetar outflows: observations at radio, X-ray, and gamma-ray wavelengths

We review observations of several classes of neutron-star-powered outflows: pulsar-wind nebulae (PWNe) inside shell supernova remnants (SNRs), PWNe interacting directly with interstellar medium (ISM), and magnetar-powered outflows. We describe radio, X-ray, and gamma-ray observations of PWNe, focusing first on integrated spectral-energy distributions (SEDs) and global spectral properties. High-resolution X-ray imaging of PWNe shows a bewildering array of morphologies, with jets, trails, and other structures. Several of the 23 so far identified magnetars show evidence for continuous or sporadic emission of material, sometimes associated with giant flares, and a few possible "magnetar-wind nebulae" have been recently identified.Comment: 61 pages, 44 figures (reduced in quality for size reasons). Published in Space Science Reviews, "Jets and Winds in Pulsar Wind Nebulae, Gamma-ray Bursts and Blazars: Physics of Extreme Energy Release