Selection for Catalytic Function with Nucleic Acids

For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself. This unit gives guidelines for design of in vitro selection experiments for catalytic function. It outlines several representative protocols as examples of successful selection experiments, providing a conceptual basis for the design and implementation of new selective‐amplification protocols for nucleic acids.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/143687/1/cpnc0904.pd

Strong Sphalerons and Electroweak Baryogenesis

We analyze the spontaneous baryogenesis and charge transport mechanisms suggested by Cohen, Kaplan and Nelson for baryon asymmetry generation in extended versions of electroweak theory. We find that accounting for non-perturbative chirality-breaking transitions due to strong sphalerons reduces the baryonic asymmetry by the factor $(m_t/\pi T)^2$ or $\alpha_W$, provided those processes are in thermal equilibrium.Comment: CERN-TH.7080/9

Mixing-induced CP violating sources for electroweak baryogenesis from a semiclassical approach

The effects of flavor mixing in electroweak baryogenesis is investigated in a generalized semiclassical WKB approach. Through calculating the nonadiabatic corrections to the particle currents it is shown that extra CP violation sources arise from the off-diagonal part of the equation of motion of particles moving inside the bubble wall. This type of mixing-induced source is of the first order in derivative expansion of the Higgs condensate, but is oscillation suppressed. The numerical importance of the mixing-induced source is discussed in the Minimal Supersymmetric Standard Model and compared with the source term induced by semiclassical force. It is found that in a large parameter space where oscillation suppression is not strong enough, the mixing-induced source can dominate over that from the semiclassical force.Comment: 19 pp, 2 figs, 1 table, some comments added, to appear in Eur.Phys.J.

Origin of Life

The evolution of life has been a big enigma despite rapid advancements in the fields of biochemistry, astrobiology, and astrophysics in recent years. The answer to this puzzle has been as mind-boggling as the riddle relating to evolution of Universe itself. Despite the fact that panspermia has gained considerable support as a viable explanation for origin of life on the Earth and elsewhere in the Universe, the issue remains far from a tangible solution. This paper examines the various prevailing hypotheses regarding origin of life like abiogenesis, RNA World, Iron-sulphur World, and panspermia; and concludes that delivery of life-bearing organic molecules by the comets in the early epoch of the Earth alone possibly was not responsible for kick-starting the process of evolution of life on our planet.Comment: 32 pages, 8 figures,invited review article, minor additio

Development of endotoxin tolerance does not influence the response to a challenge with the mucosal live-attenuated influenza vaccine in humans in vivo

Introduction: The effects of bacterial infections on the response to subsequent viral infections are largely unknown. This is important to elucidate to increase insight into the pathophysiology of bacterial and viral co-infections, and to assess whether bacterial infections may influence the course of viral infections. Methods: Healthy male subjects received either bacterial endotoxin [Escherichia coli-derived lipopolysaccharide (LPS), 2 ng/kg, n = 15] or placebo (n = 15) intravenously, followed by intranasal Fluenz (live-attenuated influenza vaccine) 1 week later. Results: LPS administration resulted in increased plasma cytokine levels and development of endotoxin tolerance in vivo and ex vivo, illustrated by attenuated cytokine production upon rechallenge with LPS. Following Fluenz administration, infectivity for the Fluenz A/B strains was similar between the LPS-Fluenz and placebo-Fluenz groups (13/15 subjects in both groups). Also, the Fluenz-induced increase in temperature and IL-6, G-CSF and IP-10 concentrations in nasal wash were similar between both groups. Conclusion: While endotoxemia profoundly attenuates the immune response upon a second LPS challenge, it does not influence the Fluenz-induced immune response. These results suggest immune suppression after bacterial infection does not alter the response to a subsequent viral infection

Tune in to your emotions: a robust personalized affective music player

The emotional power of music is exploited in a personalized affective music player (AMP) that selects music for mood enhancement. A biosignal approach is used to measure listeners’ personal emotional reactions to their own music as input for affective user models. Regression and kernel density estimation are applied to model the physiological changes the music elicits. Using these models, personalized music selections based on an affective goal state can be made. The AMP was validated in real-world trials over the course of several weeks. Results show that our models can cope with noisy situations and handle large inter-individual differences in the music domain. The AMP augments music listening where its techniques enable automated affect guidance. Our approach provides valuable insights for affective computing and user modeling, for which the AMP is a suitable carrier application

Amplification of hypercharge electromagnetic fields by a cosmological pseudoscalar

If, in addition to the standard model fields, a new pseudoscalar field exists and couples to hypercharge topological number density, it can exponentially amplify hyperelectric and hypermagnetic fields in the symmetric phase of the electroweak plasma, while coherently rolling or oscillating. We present the equations describing the coupled system of a pseudoscalar field and hypercharge electromagnetic fields in the electroweak plasma at temperatures above the electroweak phase transition, discuss approximations to the equations, and their validity. We then solve the approximate equations using assorted analytical and numerical methods, and determine the parameters for which hypercharge electromagnetic fields can be exponentially amplified.Comment: 14 pages, 6 figure

Dynamics of Two Higgs Doublet CP Violation and Baryogenesis at the Electroweak Phase Transition

We quantitatively study the charge transport mechanism of electroweak baryogenesis in a realistic two-Higgs-doublet model, comparing the contributions from quarks and leptons reflecting from electroweak domain walls, and comparing the exact profile of the CP-violating phase with a commonly used ansatz. We note that the phenomenon of spontaneous CP violation at high temperature can occur in this model, even when there is no CP violation at zero temperature. We include all known effects which are likely to influence the baryon production rate, including strong sphalerons, the nontrivial dispersion relations of the quasiparticles in the plasma, and Debye screening of gauged charges. We confirm the claim of Joyce, Prokopec and Turok that the reflection of tau leptons from the wall gives the dominant effect. We conclude that this mechanism is marginally strong enough to produce the observed baryon asymmetry of the universe.Comment: 49 pp. latex, 6 figures; section on diffusion expanded and corrected, published versio

Simulating Evolution’s First Steps

Abstract. We demonstrate a simple artificial chemistry environment in which two small evolutionary transitions from the simplest self-replicators to larger ones are observed. The replicators adapt to increasingly harsh environments, where they must synthesise the components they need for replication. The evolution of a biosynthetic pathway of increasing length is thus achieved, through the use of simple chemical rules for catalytic action.