Microconfined flow behavior of red blood cells by image analysis techniques

This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community, www.nanopaprika.eu.Red blood cells (RBCs) perform essential functions in human body, such as gas exchange between blood and tissues, thanks to their ability to deform and flow in the microvascular network. The high RBC deformability is mainly due to the viscoelastic properties of the cell membrane. Since an impaired RBC deformability could be found in some diseases, such as malaria, sickle cell anemia, diabetes and hereditary disorders, there is the need to provide further insight into measurement of RBC deformability in a physiologically-relevant flow field. Here, we report on an imaging-based in vitro systematic microfluidic investigation of RBCs flowing either in microcapillaries or in a microcirculation-mimicking device containing a network of microchannels of diameter comparable to cell size. RBC membrane shear elastic modulus and surface viscosity have been investigated by using diverging channels, while RBC time recovery constant have been measured in start-up experiments. Moreover, RBC volume and surface area have been measured in microcapillary flow. The comprehension of the single cell behavior led to the analysis of the RBC flow-induced clustering. Overall, our results provide a novel technique to estimate RBC deformability, that can be used for the analysis of pathological RBCs, for which reliable quantitative methods are still lacking

Three-systems for visual numerosity: A single case study

Abstract Humans possess the remarkable capacity to assess the numerosity of a set of items over a wide range of conditions, from a handful of items to hundreds of them. Recent evidence is starting to show that judgments over such a large range is possible because of the presence of three mechanisms, each tailored to specific stimulation conditions. Previous evidence in favour of this theory comes from the fact that discrimination thresholds and estimation reaction times are not constants across numerosity levels. Likewise, attention is capable of dissociating the three mechanisms: when healthy adult observers are asked to perform concurrently a taxing task, the judgments of low numerosities

Voice assistants in hospital triage operations

This paper analyzes the creation and usage of a voice assistant for the triage of emergency room patients. This human-centred intelligent system strongly relies on Mycroft, an extensible open source voice assistant. The patients are able to declare their symptoms to the agent, which recognizes the urgency and acts accordingly. The software can even provide useful medical informations to the users

Microfluidic mixing of low viscosity Boger fluids

This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community, www.nanopaprika.eu.This study is focused on the development of low viscosity Boger fluids and on the investigation of their elasticity on emulsion formation. Non-Newtonian continuous phases (Boger fluids) made of two different molecular weight Polyacrylamide in water plus glycerol solutions were used. While, as Newtonian continuous phase, a water plus glycerol solution showing the same viscosity as the non-Newtonian one was prepared and as dispersed phase silicon oil was used. Visualization of these emulsions flowing through a micromixer was useful in order to extract quantitative informations of their behavior, such as the velocity profile and droplets’ size distribution. Then the formation of vortex upstream of a divergent-convergent configuration has been shown as the wall migration effect, which drives droplets away from the walls and toward the center of the microcapillary investigated

Emotion-based analysis of programming languages on Stack Overflow

When developing a software engineering project, selecting the most appropriate programming language is a crucial step. Most often, feeling at ease with the possible options becomes almost as relevant as the technical features of the language. Therefore, it appears to be worth analyzing the role that the emotional component plays in this process. In this article, we analyze the trend of the emotions expressed by developers in 2018 on the Stack Overflow platform in posts concerning 26 programming languages. To do so, we propose a learning model trained by distant supervision and the comparison of two different classifier architectures

"bisphenol a: An emerging threat to male fertility"

Background: Among the factors causing male infertility, one of the most debated is the exposure to environmental contaminants. Recently, the chemical compound Bisphenol A (BPA) has drawn attention from the reproductive science community, due to its ubiquitous presence in day-to-day life. Its toxic action appears to mainly affect the male reproductive system, directly impacting male fertility. Main: The purpose of this review is to investigate current research data on BPA, providing an overview of the findings obtained from studies in animal and human models, as well as on its supposed mechanisms of action. Conclusion: A clear understanding of BPA action mechanisms, as well as the presumed risks deriving from its exposure, is becoming crucial to preserve male fertility. The development and validation of methodologies to detect BPA toxic effects on reproductive organs can provide greater awareness of the potential threat that this chemical represents

Microfluidic interactions between red blood cells and drug carriers by image analysis techniques

This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community, www.nanopaprika.eu.Blood is a complex biological fluid composed of deformable cells and platelets suspended in plasma, a protein-rich liquid. The peculiar nature of blood needs to be considered when designing a drug delivery strategy based on systemically administered carriers. Here, we report on an in vitro fluid dynamic investigation of the influence of the microcapillary flow of red blood cells (RBCs) on micron sized carriers by high speed imaging methods. The experiments were carried out in a 50μm diameter glass capillary that mimicked the hydrodynamic conditions of human microcirculation. Spherical μ particles (μ-Ps), with sizes ranging between 0.5 and 3μm, were tested. Images of the flowing RBCs and μ-Ps were acquired by a highspeed/ high-magnification microscopy. The transport and distribution of rigid particles in a suspension of RBCs under shear flow were followed for: i) the migration of RBCs towards the vessel centerline due to their deformability; ii) the cross-flow migration of μ-Ps towards the vessel wall due to their hydrodynamic interactions with RBCs; iii) the radial distribution of μ-Ps in the presence of RBCs. This study suggests that the therapeutic efficacy of μ-Ps could be ultimately affected by their interactions with the flowing RBCs in the vasculature

Mobility in Unsupervised Word Embeddings for Knowledge Extraction—The Scholars’ Trajectories across Research Topics

In the knowledge discovery field of the Big Data domain the analysis of geographic positioning and mobility information plays a key role. At the same time, in the Natural Language Processing (NLP) domain pre-trained models such as BERT and word embedding algorithms such as Word2Vec enabled a rich encoding of words that allows mapping textual data into points of an arbitrary multi-dimensional space, in which the notion of proximity reflects an association among terms or topics. The main contribution of this paper is to show how analytical tools, traditionally adopted to deal with geographic data to measure the mobility of an agent in a time interval, can also be effectively applied to extract knowledge in a semantic realm, such as a semantic space of words and topics, looking for latent trajectories that can benefit the properties of neural network latent representations. As a case study, the Scopus database was queried about works of highly cited researchers in recent years. On this basis, we performed a dynamic analysis, for measuring the Radius of Gyration as an index of the mobility of researchers across scientific topics. The semantic space is built from the automatic analysis of the paper abstracts of each author. In particular, we evaluated two different methodologies to build the semantic space and we found that Word2Vec embeddings perform better than the BERT ones for this task. Finally, The scholars’ trajectories show some latent properties of this model, which also represent new scientific contributions of this work. These properties include (i) the correlation between the scientific mobility and the achievement of scientific results, measured through the H-index; (ii) differences in the behavior of researchers working in different countries and subjects; and (iii) some interesting similarities between mobility patterns in this semantic realm and those typically observed in the case of human mobility

Fertility-sparing approach in women affected by stage i and low-grade endometrial carcinoma: An updated overview

Endometrial cancer (EC) is a deleterious condition which strongly affects a woman’s quality of life. Although aggressive interventions should be considered to treat high-grade EC, a conservative approach should be taken into consideration for women wishing to conceive. In this scenario, we present an overview about the EC fertility-sparing approach state of art. Type I EC at low stage is the only histological type which can be addressed with a fertility-sparing approach. Moreover, no myometrium and/or adnexal invasion should be seen, and lymph-vascular space should not be involved. Regarding the pharmaceutical target, progestins, in particular medroxyprogesterone acetate (MPA) or megestrol acetate (MA), are the most employed agent in conservative treatment of early-stage EC. The metformin usage and hysteroscopic assessment is still under debate, despite promising results. Particularly strict and imperious attention should be given to the follow-up and psychological wellbeing of women, especially because of the double detrimental impairment: both EC and EC-related infertility consequences