Body condition assessment using digital images.

This project assessed the ability to assign a body condition score (BCS) to a dairy cow from digital photographs or videos. Images were taken from the rear of the cow at a 0 to 20 degrees angle relative to the tail head. Four observers assigned a BCS to each of 57 cows at a farm visit (live, farm 1) and later from a photograph (photo). Means +/- standard deviations of BCS by method and observer were as follows: live = 3.25 +/- 0.51, 3.42 +/- 0.49, 3.32 +/- 0.58, 3.13 +/- 0.62; photo = 3.36 +/- 0.52, 3.32 +/- 0.43, 3.44 +/- 0.62, 3.14 +/- 0.6 for observers 1 to 4, respectively. Body condition score means differed across observers for live (observer 2 higher and observer 4 lower, compared with observers 1 and 3) and photo methods (observer 3 lower, compared with observers 1, 2, and 3); however, within observer, the mean live BCS did not differ from the mean photo BCS. Correlation coefficients between BCS assigned live and from photos were 0.84, 0.82, 0.82, and 0.90 for observers 1 to 4, respectively. Subsequently, observer 1 visited 2 farms, assigned a live BCS, and digitally photographed 187 cows (56 and 131 cows from farms 2 and 3, respectively). Observers 2, 3, and 4 assigned a BCS from the photographs. Means +/- standard deviations of BCS by observer (method) were 1 (live) 3.35 +/- 0.55; 2 (photo) 3.33 +/- 0.49; 3 (photo) 3.60 +/- 0.54; and 4 (photo) 3.26 +/- 0.62. The mean BCS for observer 3 was higher and that for observer 4 was lower than for observers 1 and 2. Correlation coefficients between observer 1 and observers 2 through 4 were 0.78, 0.76, and 0.79, respectively. Observer 1 assigned a BCS to 41 cows at a farm visit and 3 wk later assessed the BCS of cows from a video taken at a farm visit by a different individual. Cows were restrained in headlocks at a feed bunk when assessing BCS and for video production. No difference was detected for the mean BCS, for the standard deviation of the mean BCS, or in the distribution of BCS between the live and video assessments. Mean and SD for 17 groups of Holstein cows from 20 farms were used to generate 10,000 random samples of BCS. Groups of 25, 50, 100, and 150 cows were created from the random samples, and estimates of mean BCS were determined by sampling 3 to 80% of the group. Estimates of mean BCS with a sample size of 30% or more from a group of cows fell within the 95% confidence limit of the true mean more than 98% of the time. Digital photographs provide adequate imaging for assessment of BCS. Sampling 30% of a group should be adequate to assess the mean BCS. Video imaging allowed a rapid assessment of BCS but did not permit identification of individual cows

Port noise and complaints in the North Tyrrhenian sea and framework for remediation

Compared to the other relevant noise sources such as railways, roads, and airplanes, the regulation regarding port noise is lagging behind. The absence of specific laws is likely one of the main causes of the increasingly high number of complaints reported by the citizens living nearby the ports. At the same time, scientific literature concerning the impact of port noise and its mitigation is not so widespread and only a few studies are available at the moment. However, the volume of maritime traffic has increased in the last years and consequently, Port Authorities are required to assess the impact of port operations on the city soundscape without using specific directives or guidelines. In this context, the INTERREG Maritime programme projects RUMBLE, MON ACUMEN, and REPORT aim to fill this gap, by investigating the state-of-the-art of port noise in the north Tyrrhenian sea and developing helpful instruments. Data were collected via a survey sent to the Port Authorities, local environmental protection agencies and universities involved in the projects. The survey was focused on monitoring systems, previous measurement campaigns, noise maps, and citizens’ complaints already taken. The results confirmed both a lack of awareness among residents and authorities and the absence of actions aimed at reducing port noise. In this framework, the difficulties encountered by the Port Authorities in managing the ports sustainably are highlighted. An underestimation of citizens’ exposure to noise in port areas could be expected

A Multivariate Approach to Study the Bacterial Diversity Associated to the Wooden Shelves Used for Aging Traditional Sicilian Cheeses

The present study was carried to correlate the microbial diversity of the biofilms developed on the wooden boards used for aging traditional Sicilian cheeses with cheese typology. To this end, the microbial diversity of the shelves in contact with the cheeses PDO Pecorino Siciliano, PDO Piacentinu Ennese, and TAP Caciocavallo Palermitano, during ripening, was evaluated by a multivariate statistical approach. The shelf biofilms of this study were previously analyzed for their microbial composition, but no correlation between biodiversity and cheese type was investigated. Canonical discriminant analysis confirmed a cheese typology effect on the microbial loads of the wooden shelves investigated. Regarding the plate count data, the centroids of different cheeses were statistically distant from one another. This analysis also showed a good graphic separation of data regarding bacterial order operational taxonomy units (OTUs). Thus, the microbiological differences imputed to the cheese typologies were not affected by the environmental conditions of the facilities. Furthermore, wooden shelf lactic acid bacteria (LAB) were investigated for their ability to inhibit the main dairy pathogens. Although inhibitors were mainly enterococci, P. pentosaceus WS287 and W. paramesenteroides WS581 showed the highest inhibition activity, indicating their possible application to control the undesired bacteria in situ

Durability and variability of the acoustical performance of rubberized road surfaces

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Pass-by characterization of noise emitted by different categories of seagoing ships in ports

In the light of sustainability, satisfactory living conditions is an important factor for people's positive feedback in their own living environment. Acoustic comfort and noise exposure should then be carefully monitored in all human settlements. Furthermore, it is already well-known that high or prolonged noise levels may lead to unwanted health effects. Unfortunately, while in the last decades scientists and public authorities have investigated the noise produced by roads, trains, and airports, not enough efforts have been spent in studying what happens around the coastal and port areas. Following the attention brought to the subject by recent European projects on noise in port areas, the present paper characterizes the sound power level and 1/3 octave band sound power spectrum of seagoing ships while moving at low speeds. Five different categories have been distinguished: Roll-on/roll-off (RORO), container ship, oil tanker, chemical tanker, and ferry. The analysis is based on a continuous noise measurement lasting more than three months, performed in the industrial canal of the port of Livorno (Italy). The resulting noise emissions are new and useful data that could be inserted in acoustic propagation models to properly assess the noise in the areas affected by port activities. Thus, the present work can act as a supporting tool in planning ship traffic in ports towards better sustainability

Treatment of HER2+ metastatic salivary ductal carcinoma in a pregnant woman: a case report

Salivary duct carcinoma (SDC) is a rare and aggressive malignancy with a high mortality and poor response to treatment in the advanced setting. Human epidermal growth factor 2 (HER2) can be amplified in a fraction of SDC. We describe the case of HER2+ metastatic SDC of the submandibular gland in a young pregnant woman treated by multimodal treatment (chemotherapy, radiotherapy and targeted therapy). During pregnancy, a 27-year-old woman developed SDC of the left submandibular gland with lung and bone metastases. Given the HER2 overexpression, she was treated with trastuzumab, paclitaxel and cisplatin. Since the tumor had arisen during pregnancy, triptorelin was administered after delivery. A complete remission was observed, and after eight cycles of chemotherapy, radiotherapy was started in association with trastuzumab and triptorelin. A prolonged disease control and complete visceral remission were observed. Multimodal therapy based on patient's tumor characteristics showed good clinical efficacy in the treatment of metastatic SDC

Parameters affecting noise emitted by ships moving in port areas

Only recently has noise been considered in the assessment of the sustainability of port infrastructures, after decades of unawareness. INTERREG Maritime projects unveiled problems that have been neglected so far, such as the lack of proper regulation and noise exposure assessments for citizens. While it is true that a port area includes a huge variety of possible noise sources, very few of them have been characterized from an acoustical point of view. INTERREG projects have boosted research in the field, and previous studies have dealt with noise produced by moving ships in ports. The present work starts from a previous measurement campaign used to obtain broadband and 1/3-octave-band noise emissions of moving ships, and it aims to explain their uncertainties. More than a month’s worth of continuous acoustic measurements and video recordings were deeply analyzed in order to obtain an input database that is as precise as possible. A multiple regression analysis was performed in order to understand the influence that parameters such as minimum distance, speed, and draught have on ships’ noise emissions, which were calculated using pass-by measurements, with a special focus on ferries. The minimum distance of each ship’s passage from the microphone was measured using a video recording with an innovative methodology, providing results with 3 m of uncertainty. Knowing which parameter is more influential would help in planning proper measurements for monitoring or for drafting correct guidelines. Draught was determined to be uninfluential in ferries’ noise emissions, while the minimum distance and speed relations were estimated and accounted for in the calculation of a refined sound power level. From a spectrum point of view, the frequencies from 500 Hz to 2.5 kHz were determined to be those that contributed the most to the noise produced by the transit of a ship, and they vary with speed. With the studied corrections, different ferry models resulted in similar noise emissions. The standard deviation of noise emitted was reduced by 0.5 dB (A), and the average was also improved by positioning the ships’ flow at the correct average minimum distance. Furthermore, the right placement of a source is also important in the acoustic mapping phase for a correct evaluation of the propagation of noise at a distance. The use of more precise input data is important for improving the output of acoustic propagation models during the assessment of port noise in the surrounding areas

Stabilization of a p-u sensor mounted on a vehicle for measuring the acoustic impedance of road surfaces

The knowledge of the acoustic impedance of a material allows for the calculation of its acoustic absorption. Impedance can also be linked to structural and physical proprieties of materials. However, while the impedance of pavement samples in laboratory conditions can usually be measured with high accuracy using devices such as the impedance tube, complete in-situ evaluation results are less accurate than the laboratory results and is so time consuming that a full scale implementation of in-situ evaluations is practically impossible. Such a system could provide information on the homogeneity and the correct laying of an installation, which is proven to be directly linked to its acoustic emission properties. The present work studies the development of a measurement instrument which can be fastened through holding elements to a moving laboratory (i.e., a vehicle). This device overcomes the issues that afflict traditional in-situ measurements, such as the impossibility to perform a continuous spatial characterization of a given pavement in order to yield a direct evaluation of the surface’s quality. The instrumentation has been uncoupled from the vehicle’s frame with a system including a Proportional Integral Derivative (PID) controller, studied to maintain the system at a fixed distance from the ground and to reduce damping. The stabilization of this device and the measurement system itself are evaluated and compared to the traditional one

Alkaline phosphatase survey in pecorino siciliano PDO cheese

The determination of alkaline phosphatase (ALP) in cheeses has become an official method for controlling cheeses with a protected designation of origin (PDO), all of which use raw milk. PDO cheeses, characterized by high craftsmanship, usually have an uneven quality. However, for these cheeses, it is necessary to establish ALP values so that they can be defined as a raw milk product. In this study, a dataset with Pecorino Siciliano PDO samples was analyzed to determine ALP both at the core and under the rind. The results showed that there was no significant difference between the different zones in Pecorino cheese. A second dataset of 100 pecorino cheese samples determined that ALP was only at the core of the cheese. Moreover, there was a statistically significant difference between the ALP values of cheeses produced with raw milk and those produced with pasteurized milk. Furthermore, according to the temperatures, a wide variability of ALP values was observed in the Pecorino Siciliano PDO samples from the core of the cheeses. This was a result of several under scotta whey cooking methodologies adopted by cheesemakers, which do not permit a clear range. Therefore, further investigation is desirable