Detection of HIV-1 infection in dried blood spots from a 12-year-old ABO bedside test card

Background and Objectives: We tested dried blood from an ABO bedside test card which had been stored at room temperature for 12 years, to prove that a patient with HIV-1 infection had been infected by blood transfusion. Materials and Methods: Immunoblots for HIV-1 antibodies and threefold PCRs with half-nested primers for the HIV-1 integrase gene were done with eluates from the dried blood spots. Results: HIV-1 antibodies and HIV-1 DNA could be detected in the sample from one unit of blood, but not from the two other units or from the recipient before transfusion. Conclusion: Further studies should be done on the validity of stored dried blood as an alternative to the storage of frozen donor serum for several years for `look-back' studies

Magnetic properties of HO2 thin films

We report on the magnetic and transport studies of hafnium oxide thin films grown by pulsed-laser deposition on sapphire substrates under different oxygen pressures, ranging from 10-7 to 10-1 mbar. Some physical properties of these thin films appear to depend on the oxygen pressure during growth: the film grown at low oxygen pressure (P ~= 10-7 mbar) has a metallic aspect and is conducting, with a positive Hall signal, while those grown under higher oxygen pressures (7 x 10-5 <= P <= 0.4 mbar) are insulating. However, no intrinsic ferromagnetic signal could be attributed to the HfO2 films, irrespective of the oxygen pressure during the deposition.Comment: 1

Hybrid protoneutron stars with the MIT bag model

We study the hadron-quark phase transition in the interior of protoneutron stars. For the hadronic sector, we use a microscopic equation of state involving nucleons and hyperons derived within the finite-temperature Brueckner-Bethe-Goldstone many-body theory, with realistic two-body and three-body forces. For the description of quark matter, we employ the MIT bag model both with a constant and a density-dependent bag parameter. We calculate the structure of protostars with the equation of state comprising both phases and find maximum masses below 1.6 solar masses. Metastable heavy hybrid protostars are not found.Comment: 12 pages, 9 figures submitted to Phys. Rev.

Io: IUE observations of its atmosphere and the plasma torus

Two of the main components of the atmosphere of Io, neutral oxygen and sulfur, were detected with the IUE. Four observations yield brightnesses that are similar, regardless of whether the upstream or the downstream sides of the torus plasma flow around Io is observed. A simple model requires the emissions to be produced by the interaction of O and S columns in the exospheric range with 2 eV electrons. Cooling of the 5 eV torus electrons is required prior to their interaction with the atmosphere of Io. Inconsistencies in the characteristics of the spectra that cannot be accounted for in this model require further analysis with improved atomic data. The Io plasma torus was monitored with the IUE. The long-term stability of the warm torus is established. The observed brightnesses were analyzed using a model of the torus, and variations of less than 30 percent in the composition are observed, the quantitative results being model dependent

Survey for Transiting Extrasolar Planets in Stellar Systems. II. Spectrophotometry and Metallicities of Open Clusters

We present metallicity estimates for seven open clusters based on spectrophotometric indices from moderate-resolution spectroscopy. Observations of field giants of known metallicity provide a correlation between the spectroscopic indices and the metallicity of open cluster giants. We use \chi^2 analysis to fit the relation of spectrophotometric indices to metallicity in field giants. The resulting function allows an estimate of the target-cluster giants' metallicities with an error in the method of \pm0.08 dex. We derive the following metallicities for the seven open clusters: NGC 1245, [m/H]=-0.14\pm0.04; NGC 2099, [m/H]=+0.05\pm0.05; NGC 2324, [m/H]=-0.06\pm0.04; NGC 2539, [m/H]=-0.04\pm0.03; NGC 2682 (M67), [m/H]=-0.05\pm0.02; NGC 6705, [m/H]=+0.14\pm0.08; NGC 6819, [m/H]=-0.07\pm0.12. These metallicity estimates will be useful in planning future extra-solar planet transit searches since planets may form more readily in metal-rich environments.Comment: 38 pages, including 12 figures. Accepted for publication in A

First High Contrast Imaging Using a Gaussian Aperture Pupil Mask

Placing a pupil mask with a gaussian aperture into the optical train of current telescopes represents a way to attain high contrast imaging that potentially improves contrast by orders of magnitude compared to current techniques. We present here the first observations ever using a gaussian aperture pupil mask (GAPM) on the Penn State near-IR Imager and Spectrograph (PIRIS) at the Mt. Wilson 100$^{\prime\prime}$ telescope. Two nearby stars were observed, $\epsilon$ Eridani and $\mu$ Her A. A faint companion was detected around $\mu$ Her A, confirming it as a proper motion companion. Furthermore, the observed H and K magnitudes of the companion were used to constrain its nature. No companions or faint structure were observed for $\epsilon$ Eridani. We found that our observations with the GAPM achieved contrast levels similar to our coronographic images, without blocking light from the central star. The mask's performance also nearly reached sensitivities reported for other ground based adaptive optics coronographs and deep HST images, but did not reach theoretically predicted contrast levels. We outline ways that could improve the performance of the GAPM by an order of magnitude or more.Comment: 8 pages, 4 figures, accepted by ApJ letter

Library of medium-resolution fiber optic echelle spectra of F, G, K, and M field dwarfs to giants stars

We present a library of Penn State Fiber Optic Echelle (FOE) observations of a sample of field stars with spectral types F to M and luminosity classes V to I. The spectral coverage is from 3800 AA to 10000 AA with nominal a resolving power 12000. These spectra include many of the spectral lines most widely used as optical and near-infrared indicators of chromospheric activity such as the Balmer lines (H_alpha, H_beta), Ca II H & K, Mg I b triplet, Na I D_{1} and D_{2}, He I D_{3}, and Ca II IRT lines. There are also a large number of photospheric lines, which can also be affected by chromospheric activity, and temperature sensitive photospheric features such as TiO bands. The spectra have been compiled with the goal of providing a set of standards observed at medium resolution. We have extensively used such data for the study of active chromosphere stars by applying a spectral subtraction technique. However, the data set presented here can also be utilized in a wide variety of ways ranging from radial velocity templates to study of variable stars and stellar population synthesis. This library can also be used for spectral classification purposes and determination of atmospheric parameters (T_eff, log{g}, [Fe/H]). A digital version of all the fully reduced spectra is available via ftp and the World Wide Web (WWW) in FITS format.Comment: Latex file with 17 pages, 4 figures. Full postscript (text and figures) available at http://www.ucm.es/info/Astrof/fgkmsl/FOEfgkmsl.html To be published in ApJ

HST/STIS Ultraviolet Imaging of Polar Aurora on Ganymede

We report new observations of the spectrum of Ganymede in the spectral range 1160 - 1720 A made with the Space Telescope Imaging Spectrograph (STIS) on HST on 1998 October 30. The observations were undertaken to locate the regions of the atomic oxygen emissions at 1304 and 1356 A, previously observed with the GHRS on HST, that Hall et al. (1998) claimed indicated the presence of polar aurorae on Ganymede. The use of the 2" wide STIS slit, slightly wider than the disk diameter of Ganymede, produced objective spectra with images of the two oxygen emissions clearly separated. The OI emissions appear in both hemispheres, at latitudes above 40 degrees, in accordance with recent Galileo magnetometer data that indicate the presence of an intrinsic magnetic field such that Jovian magnetic field lines are linked to the surface of Ganymede only at high latitudes. Both the brightness and relative north-south intensity of the emissions varied considerably over the four contiguous orbits (5.5 hours) of observation, presumably due to the changing Jovian plasma environment at Ganymede. However, the observed longitudinal non-uniformity in the emission brightness at high latitudes, particularly in the southern hemisphere, and the lack of pronounced limb brightening near the poles are difficult to understand with current models. In addition to observed solar HI Lyman-alpha reflected from the disk, extended Lyman-alpha emission resonantly scattered from a hydrogen exosphere is detected out to beyond two Ganymede radii from the limb, and its brightness is consistent with the Galileo UVS measurements of Barth et al. (1997).Comment: 7 pages, 4 figures, accepted for publication in ApJ, June 1, 200

Identification of Fibroblasts as a Major Site of Albumin Catabolism in Peripheral Tissues

Rat serum albumin has been labeledw ith dilactitollZ5I- tyramine,( 12‘I-DLT) a radioactive tracer which remains entrappedw ithin lysosomes following cellular uptake and degradation of the carrier protein. Similar kinetics of clearance from the rat circulation were observed for albumin labeled conventionally with lZsI or 12‘I-DLT-albumin, both proteinhsa ving circulating half-lives of -2.2 days. In contrast, the recovery of whole body radioactivity had half-lives of -2.2 and 5.1 days, respectively, for the two protein preparations, indicating substantial retention of degradation products derived from catabolism of ”‘I-DLT-albumin. Measurement of total and acid-soluble radioactivity in tissues 2 or 4 days after injection of ”‘1-DLTalbumin revealed that skin and muscle accounted for the largest fraction( 50-60%)o f degradation products in the body. Fibroblaswtse re identified by autoradiography as the major cell type containing radioactive degradation products in skin and muscle. Fibroblasts were isolated from skin by collagenase digestion, followed by density gradient centrifugation. The amount of acid-soluble radioactivity recovered in these cells was in excellent agreement with that predicted based on acid precipitation of solubilized wholsek in preparations. These studies demonstrate for the first time that fibroblasts are a major cell type involved in the degradation of albuminin vivo