The aromatic nature of residue 66 of the 11-kDa subunit of ubiquinol-cytochrome <i>c</i> oxidoreductase of the yeast <i>Saccharomyces cerevisiae</i> is important for the assembly of a functional enzyme

AbstractTransformation of multi- and single-copy plasmids carrying a mutated version (LTN2, region 66-YWYWW-70 replaced by SASAA) of QCR8, the gene encoding the 11-kDa subunit of ubiquinol-cytochrome c oxidoreductase of Saccharomyces cerevisiae, to a QCR80 strain indicated the importance of this aromatic region for the assembly of a functional enzyme. Sequencing of plasmids giving spontaneous restoration of growth to some colonies among the single-copy LTN2 transformants showed that changing the sequence SASAA into the sequence FASAA could, to a large extent, overcome the observed assembly defect, indicating the importance of the aromatic nature of residue 66

Japanese investment on the Gold Coast : the interface of globalization and locality

This study explored the interface between the forces of globalization and a given place, at a given time, the Gold Coast during the 1980s. The global economic boom of the 1980s was one in which the role of Japan was particularly important. In less than half a decade capital flows from Japan surged to make it the world\u27s largest investor. Locations in the Pacific Basin were favoured destinations for Japanese investment, one of the most significant was the Gold Coast. Japanese capital and tourism helped transform its urban area from a national resort to an international tourist destination and resort centre, The surge of capital arriving to the Gold Coast was a function of economic conditions in Japan, as was its steep reduction after November 1989, Thus the Gold Coast became integrated into global capital flows and so dependent on decisions made in Tokyo, one of the main financial centres of the world. However this study has also sought to explore a more complex reality; namely, that this place also became the interface of complex cultural forces and perceptions. The wealth of the Japanese investors on the Gold Coast enabled them to realize their dream of developing projects in the most fashionable global styles. These styles were essentially Western, and it was onto these that their Japanese owners ascribed their own meanings; meanings that reflected the cultural baggage that they had brought from Japan, and through which were filtered the economic and environmental realities of the Gold Coast. The Gold Coast as locality also included residents. Hence it became an interface between two different groups of people, the Japanese and the strongly Anglo-Celtic local community. Some in the local community perceived the Japanese presence as a threat to their perception of the Gold Coast, in fact, a threat to their perception of Australia\u27s national identity. A campaign based on the politics of memory of the Japanese developed on the Gold Coast. Within weeks it became a national debate in which isolationalist, if not xenophobic traditionalists, concentrated on the Gold Coast challenged the economic rationalism and multicultural tolerance of the self-interested and ideologically convinced advocates of globalization. Governments at all levels sought to arbitrate, to legitimize standpoints, but more often than not were seen to move into positions of ineffectual flexibility. The forces of globalization on the Gold Coast were catalysts for change that in turn provoked local opposition which rapidly became a debate about national identity and direction. It is in the exploration of the complex and contradictory economic, cultural and political forces engendered by globalization that this study has sought to make a distinctive contribution

Eclipse timing variation analysis of OGLE-IV eclipsing binaries towards the Galactic Bulge – I. Hierarchical triple system candidates

We report a study of the eclipse timing variation (ETV) of short period ($P_1\le 6^d$) eclipsing binaries (EB) monitored during the photometric survey OGLE-IV. From the 425\,193 EBs we selected approximately 80\,000 binaries that we found suitable for further examination. Among them we identified 992 potential hierarchical triple (or multiple ) system candidates exhibiting light-travel-time effect (LTTE). Besides, we obtained the orbital parameters of these systems and carried out statistical analyses on the properties of these candidates. We found that (i) there is a significant lack of triple systems where the outer period is less than 500 days; (ii) the distribution of the outer eccentricities has a maximum around $e_2\approx0.3$; (iii) the outer mass ratio calculated from an estimated minimum mass of the third component is lower than $q_2\sim 0.5$ for the majority of the sample. We also present some systems that deserve special attention. (i) There are four candidates that show double periodic ETV, which we explain by the presence of a fourth companion. (ii) For two systems the perturbations of the third component are also found to be significant therefore we give a combined dynamical and LTTE ETV solution. (iii) For one system the third component is found to be probably in the substellar mass domain.Comment: Accepted for publication in MNRA

Overtone and multi-mode RR Lyrae stars in the globular cluster M3

The overtone and multi-mode RR Lyrae stars in the globular cluster M3 are studied using a 200-d long, $B,V$ and $I_{\mathrm C}$ time-series photometry obtained in 2012. 70\% of the 52 overtone variables observed show some kind of multi-periodicity (additional frequency at ${f_{0.61}}={f_{\mathrm {1O}}}/0.61$ frequency ratio, Blazhko effect, double/multi-mode pulsation, period doubling). A signal at 0.587 frequency ratio to the fundamental-mode frequency is detected in the double-mode star, V13, which may be identified as the second radial overtone mode. If this mode-identification is correct, than V13 is the first RR Lyrae star showing triple-mode pulsation of the first three radial modes. Either the Blazhko effect or the ${f_{0.61}}$ frequency (or both of these phenomena) appear in 7 double-mode stars. The $P_{\mathrm{1O}}/P_{\mathrm{F}}$ period ratio of RRd stars showing the Blazhko effect are anomalous. A displacement of the main frequency component at the fundamental-mode with the value of modulation frequency (or its half) is detected in three Blazhko RRd stars parallel with the appearance of the overtone-mode pulsation. The ${f_{0.61}}$ frequency appears in RRc stars that lie at the blue side of the double-mode region and in RRd stars, raising the suspicion that its occurrence may be connected to double-mode pulsation. The changes of the Blazhko and double-mode properties of the stars are also reviewed using the recent and archive photometric data.Comment: accepted for publication in ApJ Suppl. 26 pages, 25 figure

Electron spin-orbit splitting in InGaAs/InP quantum well studied by means of the weak antilocalization and spin-zero effects in tilted magnetic fields

The coupling between Zeeman spin splitting and Rashba spin-orbit terms has been studied experimentally in a gated InGaAs/InP quantum well structure by means of simultaneous measurements of the weak antilocalization (WAL) effect and beating in the SdH oscillations. The strength of the Zeeman splitting was regulated by tilting the magnetic field with the spin-zeros in the SdH oscillations, which are not always present, being enhanced by the tilt. In tilted fields the spin-orbit and Zeeman splittings are not additive, and a simple expression is given for the energy levels. The Rashba parameter and the electron g-factor were extracted from the position of the spin zeros in tilted fields. A good agreement is obtained for the spin-orbit coupling strength from the spin-zeros and weak antilocalization measurements.Comment: Accepted for publication in Semiconductors Science and Technolog