Functional Microbial Features Driving Community Assembly During Seed Germination and Emergence

Microbial interactions occurring on and around seeds are especially important for plant fitness since seed-borne microorganisms are the initial source of inoculum for the plant microbiota. In this study, we analyze structural and functional changes occurring within the plant microbiota at these early stages of the plant cycle, namely germination and emergence. To this purpose, we performed shotgun DNA sequencing of microbial assemblages associated to seeds, germinating seeds and seedlings of two plant species: bean and radish. We observed an enrichment of and during emergence and a set of functional traits linked to copiotrophy that could be responsible for this selection as a result of an increase of nutrient availability after germination. Representative bacterial isolates of taxa that are selected in seedlings showed indeed faster bacterial growth rate in comparison to seed-associated bacteria isolates. Finally, binning of metagenomics contigs results in the reconstruction of population genomes of the major bacterial taxa associated to the samples. Together, our results demonstrate that, although seed microbiota varied across plant species, nutrient availability during germination elicits changes of the composition of microbial communities by potentially selecting microbial groups with functional traits linked to copiotrophy. The data presented here represents the first attempts to empirically assess changes in the microbial community during plant emergence and moves us toward a more holistic understanding of the plant microbiome

Lac Blanc Pass: a natural wind-tunnel for studying drifting snow at 2700ma.s.l

International audienceThe investigation of the spatial variability of snow depth in high alpine areas is an important topic in snow hydrology, glacier and avalanche research and the transport of snow by wind is an important process for the distribution of snow in mountainous regions. That's why, for 25 years IRSTEA (previously Cemagref) and Météo France (Centre for the Study of Snow) have joined together in studying drifting snow at Col du Lac Blanc 2700 m a.s.l. near the Alpe d'Huez ski resort in the French Alps. Initially, the site was mainly equipped with conventional meteorological stations and a network of snow poles, in order to test numerical models of drifting snow Sytron (CEN) and NEMO (Cemagref). These models are complementary in terms of spatial and temporal scales: outputs of Sytron model will form the inputs of NEMO model. Then new sensors and technologies appeared which allow to develop new knowledge dealing with thresholds velocity according to morphological features of snow grains, snow flux profiles including parameters such as fall velocity and Schmidt number, histograms of particle widths, aerodynamic roughness, gust factors. More recently, the coupled snowpack/ atmosphere model Meso-NH/Crocus has been evaluated at the experimental site. At the same time, some tested sensors have been deployed in Adelie Land in Antarctica, where blowing snow accounts for a major component of the surface mass balance. Japanese and Austrian research teams have been accomodated at Lac Blanc Pass and new foreign teams are welcome. Initial observations continue. That's why Lac Blanc Pass is also a climatological reference for 25 years at 2700 m. Data are available

Characterization of the boundary layer at Dome C (East Antarctica) during the OPALE summer campaign

The regional climate model MAR was run for the region of Dome C located on the East Antarctic plateau, during Antarctic summer 2011–2012, in order to refine our understanding of meteorological conditions during the OPALE observation campaign. A very high vertical resolution is set up in the lower troposphere, with a grid spacing of roughly 2 m. Comparisons are made with observed temperatures and winds near the surface and from a 45 m high tower as well as sodar and radiation data. MAR is generally in very good agreement with the observations but sometimes underestimates cloud formation, leading to an underestimation of the simulated downward long-wave radiation. Absorbed short-wave radiation may also be slightly overestimated due to an underestimation of the snow albedo and this influences the surface energy budget and atmospheric turbulence. Nevertheless the model provides sufficiently reliable information that represent key parameters when discussing the representativeness of chemical measurements made nearby the ground surface during field campaigns conducted at the Concordia site located at Dome C (3233 m a.s.l.)

An examination of the precipitation delivery mechanisms for Dolleman Island, eastern Antarctic Peninsula

Copyright @ 2004 Wiley-BlackwellThe variability of size and source of significant precipitation events were studied at an Antarctic ice core drilling site: Dolleman Island (DI), located on the eastern coast of the Antarctic Peninsula. Significant precipitation events that occur at DI were temporally located in the European Centre for Medium-Range Weather Forecasting (ECMWF) reanalysis data set, ERA-40. The annual and summer precipitation totals from ERA-40 at DI both show significant increases over the reanalysis period. Three-dimensional backwards air parcel trajectories were then run for 5 d using the ECMWF ERA-15 wind fields. Cluster analyses were performed on two sets of these backwards trajectories: all days in the range 1979–1992 (the climatological time-scale) and a subset of days when a significant precipitation event occurred. The principal air mass sources and delivery mechanisms were found to be the Weddell Sea via lee cyclogenesis, the South Atlantic when there was a weak circumpolar trough (CPT) and the South Pacific when the CPT was deep. The occurrence of precipitation bearing air masses arriving via a strong CPT was found to have a significant correlation with the southern annular mode (SAM); however, the arrival of air masses from the same region over the climatological time-scale showed no such correlation. Despite the dominance in both groups of back trajectories of the westerly circulation around Antarctica, some other key patterns were identified. Most notably there was a higher frequency of lee cyclogenesis events in the significant precipitation trajectories compared to the climatological time-scale. There was also a tendency for precipitation trajectories to come from more northerly latitudes, mostly from 50–70°S. The El Niño Southern Oscillation (ENSO) was found to have a strong influence on the mechanism by which the precipitation was delivered; the frequency of occurrence of precipitation from the east (west) of DI increased during El Niño (La Niña) events

How a plantar pressure-based, tongue-placed tactile biofeedback modifies postural control mechanisms during quiet standing

The purpose of the present study was to determine the effects of a plantar pressure-based, tongue-placed tactile biofeedback on postural control mechanisms during quiet standing. To this aim, sixteen young healthy adults were asked to stand as immobile as possible with their eyes closed in two conditions of No-biofeedback and Biofeedback. Centre of foot pressure (CoP) displacements, recorded using a force platform, were used to compute the horizontal displacements of the vertical projection the centre of gravity (CoGh) and those of the difference between the CoP and the vertical projection of the CoG (CoP-CoGv). Altogether, the present findings suggest that the main way the plantar pressure-based, tongue-placed tactile biofeedback improves postural control during quiet standing is via both a reduction of the correction thresholds and an increased efficiency of the corrective mechanism involving the CoGh displacements

Оценка осадков в прибрежных районах Антарктики в глобальной модели атмосферы LMDZ6 с использованием наземных радиолокационных наблюдений

In the current context of climate change in the poles, one of the objectives of the APRES3 (Antarctic Precipitation Remote Sensing from Surface and Space) project was to characterize the vertical structure of precipitation in order to better simulate it. Precipitation simulated by models in Antarctica is currently very widespread and it overestimates the data. Sensitivity studies have been conducted using a global climate model and compared to the observations obtained at the Dumont d’Urville coast station, obtained by a Micro Rain Radar (MRR). The LMDz/IPSL general circulation model, with zoomed configuration over Dumont d’Urville, has been considered for this study. A sensitivity study was conducted on the physical and numerical parameters of the LMDz model with the aim of estimating their contribution to the precipitation simulation. Sensitivity experiments revealed that changes in the sedimentation and sublimation parameters do not significantly impact precipitation rate. However, dissipation of the LMDz model, which is a numerical process that dissipates spatially excessive energy and keeps the model stable, impacts precipitation indirectly but very strongly. A suitable adjustment of the dissipation reduces significantly precipitation over Antarctic peripheral area, thus providing a simulated profile in better agreement with the MRR observations.В текущем тренде изменения климата на полюсах одна из задач проекта APRES3 (Дистанционное зондирование осадков в Антарктике с поверхности и из космоса) заключается в том, чтобы уточнить вертикальную структуру осадков и повысить качество их прогноза. Известные результаты моделирования осадков в Антарктиде базируются на данных с высокой степенью неопределенности и сильно разнятся. Исследование избирательной чувствительности расчета осадков проводилось на основе глобальной климатической модели и сопоставлялось с наблюдениями, полученными с помощью метеорадара (MRR) на береговой станции Дюмон-д’Юрвиль. Использовалась LMDz/IPSL-модель общей циркуляции с повышенной детализацией в районе станции Дюмон-д’Юрвиль. Была выполнена оценка вклада физических и численных параметров данной модели в расчет осадков. Вычислительные эксперименты показали, что изменения параметров седиментации и сублимации не влияют существенно на прогнозируемую скорость выпадения осадков. Однако диссипация, возникающая в модели LMDz в процессе вычислений, рассеивая пространственно избыточную энергию и обеспечивая устойчивость модели, хотя и косвенно, но очень сильно влияет на рассчитываемую величину осадков. Адекватная подгонка уровня рассеивания при моделировании значительно снижает количество осадков в периферийных районах Антарктики, обеспечивая таким образом лучшее согласование моделируемого профиля с данными метеорадарных наблюдений

Characterizing the limited use of point-of-care ultrasound in Colombian emergency medicine residencies

Background: Emergency medicine (EM) is a growing specialty in Colombia with five residency programs in the country. EM leadership is interested in incorporating point-of-care (POC) ultrasound into a standardized national EM residency curriculum. This study is a nationwide survey of Colombian EM residents designed to explore the current state of POC ultrasound use within EM residencies and examine specific barriers preventing its expansion. Methods: We conducted a mix-methodology study of all available current EM residents in the five EM residencies in Colombia. The quantitative survey assessed previous ultrasound experience, current use of various applications, desire for further training, and perceived barriers to expanded use. Focus group discussions (FGDs) were conducted with current EM residents to gather additional qualitative insight into their practice patterns and perceived barriers to clinician-performed ultrasound. Results: Sixty-nine EM residents completed the quantitative survey, a response rate of 85% of all current EM residents in Colombia; 52% of resident respondents had previously used ultrasound during their training. Of these, 58% indicated that they had performed 40 scans. The most frequently used applications indicated by respondents were trauma, obstetrics, and procedures including vascular access. A quarter indicated they had previously received some ultrasound training, but almost all expressed an interest in learning more. Significant barriers included lack of trained teachers (indicated by 78% of respondents), absence of machines (57%), and limited time (41%). In FGDs, the barriers identified were inter-specialty conflicts over the control of ultrasonography, both institutionally and nationally, and program-specific curriculum decisions to include POC ultrasound. Conclusion: While currently limited in their access, EM residents in Colombia have a strong interest in integrating POC ultrasound into their training. Current barriers to expanded use include traditional barriers such as a lack of equipment seen in many developing countries, as well as inter-specialty conflicts typical of developed countries. Further collaboration is underway to help overcome these obstacles and integrate POC ultrasound into Colombian EM residency training

Characterizing the limited use of point-of-care ultrasound in Colombian emergency medicine residencies

Background: Emergency medicine (EM) is a growing specialty in Colombia with five residency programs in the country. EM leadership is interested in incorporating point-of-care (POC) ultrasound into a standardized national EM residency curriculum. This study is a nationwide survey of Colombian EM residents designed to explore the current state of POC ultrasound use within EM residencies and examine specific barriers preventing its expansion. Methods: We conducted a mix-methodology study of all available current EM residents in the five EM residencies in Colombia. The quantitative survey assessed previous ultrasound experience, current use of various applications, desire for further training, and perceived barriers to expanded use. Focus group discussions (FGDs) were conducted with current EM residents to gather additional qualitative insight into their practice patterns and perceived barriers to clinician-performed ultrasound. Results: Sixty-nine EM residents completed the quantitative survey, a response rate of 85% of all current EM residents in Colombia; 52% of resident respondents had previously used ultrasound during their training. Of these, 58% indicated that they had performed <10 scans and 17% reported >40 scans. The most frequently used applications indicated by respondents were trauma, obstetrics, and procedures including vascular access. A quarter indicated they had previously received some ultrasound training, but almost all expressed an interest in learning more. Significant barriers included lack of trained teachers (indicated by 78% of respondents), absence of machines (57%), and limited time (41%). In FGDs, the barriers identified were inter-specialty conflicts over the control of ultrasonography, both institutionally and nationally, and program-specific curriculum decisions to include POC ultrasound. Conclusion: While currently limited in their access, EM residents in Colombia have a strong interest in integrating POC ultrasound into their training. Current barriers to expanded use include traditional barriers such as a lack of equipment seen in many developing countries, as well as inter-specialty conflicts typical of developed countries. Further collaboration is underway to help overcome these obstacles and integrate POC ultrasound into Colombian EM residency training. © 2014 Henwood et al.; licensee Springer