134 research outputs found

    Concept of a laser-plasma based electron source for sub-10 fs electron diffraction

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    We propose a new concept of an electron source for ultrafast electron diffraction with sub-10~fs temporal resolution. Electrons are generated in a laser-plasma accelerator, able to deliver femtosecond electron bunches at 5 MeV energy with kHz repetition rate. The possibility of producing this electron source is demonstrated using Particle-In-Cell simulations. We then use particle tracking simulations to show that this electron beam can be transported and manipulated in a realistic beamline, in order to reach parameters suitable for electron diffraction. The beamline consists of realistic static magnetic optics and introduces no temporal jitter. We demonstrate numerically that electron bunches with 5~fs duration and containing 1.5~fC per bunch can be produced, with a transverse coherence length exceeding 2~nm, as required for electron diffraction

    Galaxy And Mass Assembly (GAMA): extended intragroup light in a group at <i>z</i> = 0.2 from deep Hyper Suprime-Cam images

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    We present a pilot study to assess the potential of Hyper Suprime-Cam Public Data Release 2 (HSC-PDR2) images for the analysis of extended faint structures within groups of galaxies. We examine the intragroup light (IGL) of the group 400138 (Mdyn = 1.3 ± 0.5 × 1013 M⊙, z ∼ 0.2) from the Galaxy And Mass Assembly (GAMA) survey using Hyper Suprime-Cam Subaru Strategic Program Public Data Release 2 (HSC-SSP PDR2) images in g, r, and i bands. We present the most extended IGL measurement to date, reaching down to μlimg=30.76 mag arcsec−2 (3σ; 10 × 10 arcsec2) at a semimajor axis of 275 kpc. The IGL shows mean colour values of g − i = 0.92, g − r = 0.60, and r − i = 0.32 (±0.01). The IGL stellar populations are younger (2–2.5 Gyr) and less metal rich ([Fe/H] ∼ −0.4) than those of the host group galaxies. We find a range of IGL fractions as a function of total group luminosity of ∼2−36 per cent depending on the definition of IGL, with larger fractions the bluer the observation wavelength. The early-type to late-type galaxy ratio suggests that 400138 is a more evolved group, dominated by early-type galaxies, and the IGL fraction agrees with that of other similarly evolved groups. These results are consistent with tidal stripping of the outer parts of Milky Way-like galaxies as the main driver of the IGL build-up. This is supported by the detection of substructure in the IGL towards the galaxy member 1660615 suggesting a recent interaction (<1 Gyr ago) of that galaxy with the core of the group

    Galaxy And Mass Assembly (GAMA): extended intragroup light in a group at z = 0.2 from deep Hyper Suprime-Cam images

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    We present a pilot study to assess the potential of Hyper Suprime-Cam Public Data Release 2 (HSC-PDR2) images for the analysis of extended faint structures within groups of galaxies. We examine the intragroup light (IGL) of the group 400138 (Mdyn = 1.3 ± 0.5 × 1013 M⊙, z ∼ 0.2) from the Galaxy And Mass Assembly (GAMA) survey using Hyper Suprime-Cam Subaru Strategic Program Public Data Release 2 (HSC-SSP PDR2) images in g, r, and i bands. We present the most extended IGL measurement to date, reaching down to μglim=30.76 role= presentation style= box-sizing: border-box; margin: 0px; padding: 0px; border: 0px; font-variant: inherit; font-stretch: inherit; line-height: normal; font-family: inherit; vertical-align: baseline; display: inline; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; position: relative; \u3eμlimg=30.76μglim=30.76 mag arcsec−2 (3σ; 10 × 10 arcsec2) at a semimajor axis of 275 kpc. The IGL shows mean colour values of g − i = 0.92, g − r = 0.60, and r − i = 0.32 (±0.01). The IGL stellar populations are younger (2–2.5 Gyr) and less metal rich ([Fe/H] ∼ −0.4) than those of the host group galaxies. We find a range of IGL fractions as a function of total group luminosity of ∼2−36 per cent role= presentation style= box-sizing: border-box; margin: 0px; padding: 0px; border: 0px; font-variant: inherit; font-stretch: inherit; line-height: normal; font-family: inherit; vertical-align: baseline; display: inline; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; position: relative; \u3e∼2−36 per cent∼2−36 per cent depending on the definition of IGL, with larger fractions the bluer the observation wavelength. The early-type to late-type galaxy ratio suggests that 400138 is a more evolved group, dominated by early-type galaxies, and the IGL fraction agrees with that of other similarly evolved groups. These results are consistent with tidal stripping of the outer parts of Milky Way-like galaxies as the main driver of the IGL build-up. This is supported by the detection of substructure in the IGL towards the galaxy member 1660615 suggesting a recent interaction (\u3c1 Gyr ago) of that galaxy with the core of the group

    Exploring glycopeptide-resistance in Staphylococcus aureus: a combined proteomics and transcriptomics approach for the identification of resistance-related markers

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    BACKGROUND: To unravel molecular targets involved in glycopeptide resistance, three isogenic strains of Staphylococcus aureus with different susceptibility levels to vancomycin or teicoplanin were subjected to whole-genome microarray-based transcription and quantitative proteomic profiling. Quantitative proteomics performed on membrane extracts showed exquisite inter-experimental reproducibility permitting the identification and relative quantification of >30% of the predicted S. aureus proteome. RESULTS: In the absence of antibiotic selection pressure, comparison of stable resistant and susceptible strains revealed 94 differentially expressed genes and 178 proteins. As expected, only partial correlation was obtained between transcriptomic and proteomic results during stationary-phase. Application of massively parallel methods identified one third of the complete proteome, a majority of which was only predicted based on genome sequencing, but never identified to date. Several over-expressed genes represent previously reported targets, while series of genes and proteins possibly involved in the glycopeptide resistance mechanism were discovered here, including regulators, global regulator attenuator, hyper-mutability factor or hypothetical proteins. Gene expression of these markers was confirmed in a collection of genetically unrelated strains showing altered susceptibility to glycopeptides. CONCLUSION: Our proteome and transcriptome analyses have been performed during stationary-phase of growth on isogenic strains showing susceptibility or intermediate level of resistance against glycopeptides. Altered susceptibility had emerged spontaneously after infection with a sensitive parental strain, thus not selected in vitro. This combined analysis allows the identification of hundreds of proteins considered, so far as hypothetical protein. In addition, this study provides not only a global picture of transcription and expression adaptations during a complex antibiotic resistance mechanism but also unravels potential drug targets or markers that are constitutively expressed by resistant strains regardless of their genetic background, amenable to be used as diagnostic targets

    Response of the photosynthetic apparatus to a flowering-inductive period by water stress in Citrus

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    The photosynthetic responses to a flowering-inductive water-stress period and recovery were studied and compared in two Citrus species. Under greenhouse conditions, Fino lemon and Owari satsuma trees were subjected to moderate (-2 MPa at predawn) and severe (-3 MPa) water stress levels and were re-watered after 60 days. Vegetative growth was inhibited during the stress assays, and strong defoliation levels were reported, especially in Fino lemon. In both species, bud sprouting was induced after re-watering. Flowers and vegetative shoots developed in Owari satsuma after a drought period, and the development was independent of the stress level. In Fino lemon, vegetative shoots and flowers were primarily formed after moderate and severe stress, respectively. The photosynthetic rate and stomatal conductance were reduced by water stress, and a marked increase in water-use efficiency at the moderate water deficit level was observed. Nevertheless, the photosynthetic apparatus was not damaged, since the maximum quantum yield, photosynthetic pigment concentrations and Rubisco level and activity did not change. Furthermore, the measured malonyldialdehyde (MDA) and peroxidase activity indicated that oxidative stress was not specifically triggered by water stress in our study. Therefore, the gas exchange, fluorescence and biochemical parameters suggested that diffusional limitations to photosynthesis predominated in both of the studied Citrus species, and explained the rapid recovery of the photosynthetic parameters after rehydration. The net CO 2 fixation rate and stomatal conductance were recovered within 24 h in Fino lemon, whereas 3 days were required in Owari satsuma. This suggests the presence of some metabolic limitations in the latter species. Furthermore, the sensibility of the defoliation rates, the accumulation of proline and the stomatal behaviour in response to water stress indicated a higher drought tolerance of Fino lemon, according to its better acclimation to hot climates. © 2011 Springer-Verlag.The authors thank Dr. J. Moreno and co-workers from the Departamento de Bioquimica of the Universidad de Valencia for his help and support in the Rubisco assays, and Dr. F. Fornes, Dr. A. Calatayud and Dr. E. Primo-Millo for the critical review of the manuscript. This work was funded by the Universitat Politecnica de Valencia, Spain (Ayudas para primeros proyectos de investigacion PAID06-06).Ávila Reséndiz, C.; Guardiola Barcena, JL.; González Nebauer, S. (2012). Response of the photosynthetic apparatus to a flowering-inductive period by water stress in Citrus. Trees - Structure and Function. 26(3):833-840. https://doi.org/10.1007/s00468-011-0657-4S833840263Addicott FT (1982) Abscission. 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    Ecological conditions, flora and vegetation of a large doline in the Mecsek Mountains (South Hungary)

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    Vegetation-environment relationships were investigated in a large doline of the Mecsek Mts (South Hungary). To reveal the vegetation pattern, we collected vegetation data and environmental variables along a 243 m long transect. Atotal of 144 vascular plant species and 4 vegetation types were identified in the doline.We found that both the species composition and the vegetation pattern are significantly influenced by air temperature, air humidity, soil moisture and altitude. Our results confirm the putative temperature and vegetation inversion in the doline
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