4,428 research outputs found
Einstein a Barcelona: ciència societat a la Catalunya d'entreguerres
Una anàlisi rigorosa de l'impacte del pensament d'Einstein a Cataluny
Michigan\u27s Binding Summary Jury Trial: Reward or Punishment - Farleigh v. Amalgamated Transit Union, Local 1251
In 1988, the Michigan Supreme Court added the summary jury trial to its arsenal of settlement devices available to trial judges.\u27 Unfortunately, the summary jury trial employed in Farleigh v. Amalgamated Transit Union, Local 1251 failed to meet its goal, and no settlement was reached by the parties.6 Nevertheless, the Michigan Court of Appeals chose to enforce the summary jury verdict,7 thereby drawing into question not only the ability of the summary jury trial to meet the preliminary goal of promoting settlement, but also the larger goal of the accomplishment of justic
Mycorrhizal-Bacterial Amelioration of Plant Abiotic and Biotic Stress
Soil microbiota plays an important role in the sustainable production of the different types of agrosystems. Among the members of the plant microbiota, mycorrhizal fungi (MF) and plant growth-promoting bacteria (PGPB) interact in rhizospheric environments leading to additive and/or synergistic effects on plant growth and heath. In this manuscript, the main mechanisms used by MF and PGPB to facilitate plant growth are reviewed, including the improvement of nutrient uptake, and the reduction of ethylene levels or biocontrol of potential pathogens, under both normal and stressful conditions due to abiotic or biotic factors. Finally, it is necessary to expand both research and field use of bioinoculants based on these components and take advantage of their beneficial interactions with plants to alleviate plant stress and improve plant growth and production to satisfy the demand for food for an ever-increasing human population
Pale Moon : An Indian Love Song
https://digitalcommons.library.umaine.edu/mmb-vp/3349/thumbnail.jp
36 degree step size of proton-driven c-ring rotation in FoF1-ATP synthase
Synthesis of the biological "energy currency molecule" adenosine triphosphate
ATP is accomplished by FoF1-ATP synthase. In the plasma membrane of Escherichia
coli, proton-driven rotation of a ring of 10 c subunits in the Fo motor powers
catalysis in the F1 motor. While F1 uses 120 degree stepping, Fo models predict
a step-by-step rotation of c subunits 36 degree at a time, which is here
demonstrated by single-molecule fluorescence resonance energy transfer.Comment: 8 pages, 1 figur
Sampling Plans for Control-Inspection Schemes Under Independent and Dependent Sampling Designs With Applications to Photovoltaics
The evaluation of produced items at the time of delivery is, in practice,
usually amended by at least one inspection at later time points. We extend the
methodology of acceptance sampling for variables for arbitrary unknown
distributions when additional sampling infor- mation is available to such
settings. Based on appropriate approximations of the operating characteristic,
we derive new acceptance sampling plans that control the overall operating
characteristic. The results cover the case of independent sampling as well as
the case of dependent sampling. In particular, we study a modified panel
sampling design and the case of spatial batch sampling. The latter is advisable
in photovoltaic field monitoring studies, since it allows to detect and analyze
local clusters of degraded or damaged modules. Some finite sample properties
are examined by a simulation study, focusing on the accuracy of estimation
Distinct functions of S. pombe Rec12 (Spo11) protein and Rec12-dependent crossover recombination (chiasmata) in meiosis I; and a requirement for Rec12 in meiosis II
BACKGROUND: In most organisms proper reductional chromosome segregation during meiosis I is strongly correlated with the presence of crossover recombination structures (chiasmata); recombination deficient mutants lack crossovers and suffer meiosis I nondisjunction. We report that these functions are separable in the fission yeast Schizosaccharomyces pombe. RESULTS: Intron mapping and expression studies confirmed that Rec12 is a member of the Spo11/Top6A topoisomerase family required for the formation of meiotic dsDNA breaks and recombination. rec12-117, rec12-D15 (null), and rec12-Y98F (active site) mutants lacked most crossover recombination and chromosomes segregated abnormally to generate aneuploid meiotic products. Since S. pombe contains only three chromosome pairs, many of those aneuploid products were viable. The types of aberrant chromosome segregation were inferred from the inheritance patterns of centromere linked markers in diploid meiotic products. The rec12-117 and rec12-D15 mutants manifest segregation errors during both meiosis I and meiosis II. Remarkably, the rec12-Y98F (active site) mutant exhibited essentially normal meiosis I segregation patterns, but still exhibited meiosis II segregation errors. CONCLUSIONS: Rec12 is a 345 amino acid protein required for most crossover recombination and for chiasmatic segregation of chromosomes during meiosis I. Rec12 also participates in a backup distributive (achiasmatic) system of chromosome segregation during meiosis I. In addition, catalytically-active Rec12 mediates some signal that is required for faithful equational segregation of chromosomes during meiosis II
Screening of bacterial endophytes able to promote plant growth and increase salinity tolerance
Bacterial endophytes can colonize plant tissues without harming the plant. Instead, they are often able to increase plant growth and tolerance to environmental stresses. In this work, new strains of bacterial endophytes were isolated from three economically important crop plants (sorghum, cucumber and tomato) grown in three different regions in soils with different management. All bacterial strains were identified by 16S rRNA sequencing and characterized for plant beneficial traits. Based on physiological activities, we selected eight strains that were further tested for their antibiotic resistance profile and for the ability to efficiently colonize the interior of sorghum plants. According to the results of the re-inoculation test, five strains were used to inoculate sorghum seeds. Then, plant growth promotion activity was assessed on sorghum plants exposed to salinity stress. Only two bacterial endophytes increased plant biomass, but three of them delayed or reduced plant salinity stress symptoms. These five strains were then characterized for the ability to produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which is involved in the increase of stress tolerance. Pseudomonas brassicacearum SVB6R1 was the only strain that was able to produce this enzyme, suggesting that ACC deaminase is not the only physiological trait involved in conferring plant tolerance to salt stress in these bacterial strains
Monitoring the rotary motors of single FoF1-ATP synthase by synchronized multi channel TCSPC
Confocal time resolved single-molecule spectroscopy using pulsed laser
excitation and synchronized multi channel time correlated single photon
counting (TCSPC) provides detailed information about the conformational changes
of a biological motor in real time. We studied the formation of adenosine
triphosphate, ATP, from ADP and phosphate by FoF1-ATP synthase. The reaction is
performed by a stepwise internal rotation of subunits of the lipid
membrane-embedded enzyme. Using fluorescence resonance energy transfer, FRET,
we detected rotation of this biological motor by sequential changes of
intramolecular distances within a single FoF1-ATP synthase. Prolonged
observation times of single enzymes were achieved by functional immobilization
to the glass surface. The stepwise rotary subunit movements were identified by
Hidden Markov Models (HMM) which were trained with single-molecule FRET
trajectories. To improve the accuracy of the HMM analysis we included the
single-molecule fluorescence lifetime of the FRET donor and used alternating
laser excitation to co-localize the FRET acceptor independently within a photon
burst. The HMM analysis yielded the orientations and dwell times of rotary
subunits during stepwise rotation. In addition, the action mode of bactericidal
drugs, i.e. inhibitors of FoF1-ATP synthase like aurovertin, could be
investigated by the time resolved single-molecule FRET approach.Comment: 12 pages, 9 figure
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