Novel African trypanocidal agents: membrane rigidifying peptides

The bloodstream developmental forms of pathogenic African trypanosomes are uniquely susceptible to killing by small hydrophobic peptides. Trypanocidal activity is conferred by peptide hydrophobicity and charge distribution and results from increased rigidity of the plasma membrane. Structural analysis of lipid-associated peptide suggests a mechanism of phospholipid clamping in which an internal hydrophobic bulge anchors the peptide in the membrane and positively charged moieties at the termini coordinate phosphates of the polar lipid headgroups. This mechanism reveals a necessary phenotype in bloodstream form African trypanosomes, high membrane fluidity, and we suggest that targeting the plasma membrane lipid bilayer as a whole may be a novel strategy for the development of new pharmaceutical agents. Additionally, the peptides we have described may be valuable tools for probing the biosynthetic machinery responsible for the unique composition and characteristics of African trypanosome plasma membranes

Expression site attenuation mechanistically links antigenic variation and development in Trypanosoma brucei

We have discovered a new mechanism of monoallelic gene expression that links antigenic variation, cell cycle, and development in the model parasite Trypanosoma brucei. African trypanosomes possess hundreds of variant surface glycoprotein (VSG) genes, but only one is expressed from a telomeric expression site (ES) at any given time. We found that the expression of a second VSG alone is sufficient to silence the active VSG gene and directionally attenuate the ES by disruptor of telomeric silencing-1B (DOT1B)-mediated histone methylation. Three conserved expression-site-associated genes (ESAGs) appear to serve as signal for ES attenuation. Their depletion causes G1-phase dormancy and reversible initiation of the slender-to-stumpy differentiation pathway. ES-attenuated slender bloodstream trypanosomes gain full developmental competence for transformation to the tsetse fly stage. This surprising connection between antigenic variation and developmental progression provides an unexpected point of attack against the deadly sleeping sickness

Fusion rate enhancement due to energy spread of colliding nuclei

Experimental results for sub-barrier nuclear fusion reactions show cross section enhancements with respect to bare nuclei which are generally larger than those expected according to electron screening calculations. We point out that energy spread of target or projectile nuclei is a mechanism which generally provides fusion enhancement. We present a general formula for calculating the enhancement factor and we provide quantitative estimate for effects due to thermal motion, vibrations inside atomic, molecular or crystal system, and due to finite beam energy width. All these effects are marginal at the energies which are presently measurable, however they have to be considered in future experiments at still lower energies. This study allows to exclude several effects as possible explanation of the observed anomalous fusion enhancements, which remain a mistery.Comment: 17 pages with 3 ps figure included. Revtex styl

Macroscopic Self-Evolution of Dynamic Hydrogels to Create Hollow Interiors

A solid-to-hollow evolution in macroscopic structures is challenging in synthetic materials. A fundamentally new strategy is reported for guiding macroscopic, unidirectional shape evolution of materials without compromising the material's integrity. This strategy is based on the creation of a field with a “swelling pole” and a “shrinking pole” to drive polymers to disassemble, migrate, and resettle in the targeted region. This concept is demonstrated using dynamic hydrogels containing anchored acrylic ligands and hydrophobic long alkyl chains. Adding water molecules and ferric ions (Fe3+) to induce a swelling–shrinking field transforms the hydrogels from solid to hollow. The strategy is versatile in the generation of various closed hollow objects (for example, spheres, helix tubes, and cubes with different diameters) for different applications

Astrophysical factors:Zero energy vs. Most effective energy

Effective astrophysical factors for non-resonant astrophysical nuclear reaction are invariably calculated with respect to a zero energy limit. In the present work that limit is shown to be very disadvantageous compared to the more natural effective energy limit. The latter is used in order to modify the thermonuclear reaction rate formula so that it takes into account both plasma and laboratory screening effects.Comment: 7 RevTex pages. Accepted for publication in Phys.Rev.

Radiation correction to astrophysical fusion reactions and the electron screening problem

We discuss the effect of electromagnetic environment on laboratory measurements of the nuclear fusion reactions of astrophysical interest. The radiation field is eliminated using the path integral formalism in order to obtain the influence functional, which we evaluate in the semi-classical approximation. We show that enhancement of the tunneling probability due to the radiation correction is extremely small and does not resolve the longstanding problem that the observed electron screening effect is significantly larger than theoretical predictions.Comment: 9 pages, 1 eps figure

Screening of Nuclear Reactions in the Sun and Solar Neutrinos

We quantitatively determine the effect and the uncertainty on solar neutrino production arising from the screening process. We present predictions for the solar neutrino fluxes and signals obtained with different screening models available in the literature and by using our stellar evolution code. We explain these numerical results in terms of simple laws relating the screening factors with the neutrino fluxes. Futhermore we explore a wider range of models for screening, obtained from the Mitler model by introducing and varying two phenomenological parameters, taking into account effects not included in the Mitler prescription. Screening implies, with respect to a no-screening case, a central temperat reduction of 0.5%, a 2% (8%) increase of Beryllium (Boron)-neutrino flux and a 2% (12%) increase of the Gallium (Chlorine) signal. We also find that uncertainties due to the screening effect ar at the level of 1% for the predicted Beryllium-neutrino flux and Gallium signal, not exceeding 3% for the Boron-neutrino flux and the Chlorine signal.Comment: postscript file 11 pages + 4 figures compressed and uuencoded we have replaced the previous paper with a uuencoded file (the text is the same) for any problem please write to [email protected]

Atomic effects in astrophysical nuclear reactions

Two models are presented for the description of the electron screening effects that appear in laboratory nuclear reactions at astrophysical energies. The two-electron screening energy of the first model agrees very well with the recent LUNA experimental result for the break-up reaction $He3(He3,2p)He^{4}$, which so far defies all available theoretical models. Moreover, multi-electron effects that enhance laboratory reactions of the CNO cycle and other advanced nuclear burning stages, are also studied by means of the Thomas-Fermi model, deriving analytical formulae that establish a lower and upper limit for the associated screening energy. The results of the second model, which show a very satisfactory compatibility with the adiabatic approximation ones, are expected to be particularly useful in future experiments for a more accurate determination of the CNO astrophysical factors.Comment: 14 RevTex pages + 2 ps (revised) figures. Phys.Rev.C (in production

Disciplining Skepticism Through Kant’s Critique, Fichte’s Idealism, and Hegel’s Negations

This chapter considers the encounter of skepticism with the Kantian and post-Kantian philosophical enterprise and focuses on the intriguing feature whereby it is assimilated into this enterprise. In this period, skepticism becomes interchangeable with its other, which helps understand the proliferation of many kinds of views under its name and which forms the background for transforming skepticism into an anonymous, routine practice of raising objections and counter-objections to one’s own view. German philosophers of this era counterpose skepticism to dogmatism and criticism, ancient to modern skepticism, and, importantly, conceptualize the transitions from one form to another, which forms the conceptual matrix in which new disciplinary forms, such as psychology, anthropology, and historicism contend for cultural-intellectual standing beside philosophy. I present this assimilationist trajectory by reviewing three well-known moments of this encounter of skepticism and idealism: (1) Kant’s idealization of skepticism as a floating position amidst various philosophical positions through the dialectic, polemics, systematics, and history of pure reason; (2) Fichte’s schematic conception of skepticism as a dispute of systems in the early Wissenschaftslehre following his review of the skeptic G. E. Schulze’s attacks on Critical philosophy; (3) Hegel’s historicizing conception of skepticism in the context of differences between subjective idealism and speculative thought and his early Jena review of another work by the same skeptic Schulze

Nuclear Reaction Network for Primordial Nucleosynthesis: a detailed analysis of rates, uncertainties and light nuclei yields

We analyze in details the standard Primordial Nucleosynthesis scenario. In particular we discuss the key theoretical issues which are involved in a detailed prediction of light nuclide abundances, as the weak reaction rates, neutrino decoupling and nuclear rate modeling. We also perform a new analysis of available data on the main nuclear processes entering the nucleosynthesis reaction network, with particular stress on their uncertainties as well as on their role in determining the corresponding uncertainties on light nuclide theoretical estimates. The current status of theoretical versus experimental results for 2H, 3He, 4He and 7Li is then discussed using the determination of the baryon density as obtained from Cosmic Microwave Background anisotropies.Comment: LaTeX, 83 pages, 30 .pdf figures. Some typos in the units of R-functions in appendix D and relative plots fixe