Microservices Validation: Methodology and Implementation

Due to the wide spread of cloud computing, arises actual question about architecture, design and implementation of cloud applications. The microservice model describes the design and development of loosely coupled cloud applications when computing resources are provided on the basis of automated IaaS and PaaS cloud platforms. Such applications consist of hundreds and thousands of service instances, so automated validation and testing of cloud applications developed on the basis of microservice model is a pressing issue. There are constantly developing new methods of testing both individual microservices and cloud applications at a whole. This article presents our vision of a framework for the validation of the microservice cloud applications, providing an integrated approach for the implementation of various testing methods of such applications, from basic unit tests to continuous stability testing

Strain- and Adsorption-Dependent Electronic States and Transport or Localization in Graphene

The chapter generalizes results on influence of uniaxial strain and adsorption on the electron states and charge transport or localization in graphene with different configurations of imperfections (point defects): resonant (neutral) adsorbed atoms either oxygen- or hydrogen-containing molecules or functional groups, vacancies or substitutional atoms, charged impurity atoms or molecules, and distortions. To observe electronic properties of graphene-admolecules system, we applied electron paramagnetic resonance technique in a broad temperature range for graphene oxides as a good basis for understanding the electrotransport properties of other active carbons. Applied technique allowed observation of possible metal-insulator transition and sorption pumping effect as well as discussion of results in relation to the granular metal model. The electronic and transport properties are calculated within the framework of the tight-binding model along with the Kubo-Greenwood quantum-mechanical formalism. Depending on electron density and type of the sites, the conductivity for correlated and ordered adsorbates is found to be enhanced in dozens of times as compared to the cases of their random distribution. In case of the uniaxially strained graphene, the presence of point defects counteracts against or contributes to the band-gap opening according to their configurations. The band-gap behaviour is found to be nonmonotonic with strain in case of a simultaneous action of defect ordering and zigzag deformation. The amount of localized charge carriers (spins) is found to be correlated with the content of adsorbed centres responsible for the formation of potential barriers and, in turn, for the localization effects. Physical and chemical states of graphene edges, especially at a uniaxial strain along one of them, play a crucial role in electrical transport phenomena in graphene-based materials.Comment: 16 pages, 10 figure

Combined Matrix-Isolation Infrared and Theoretical DFT and ab Initio Study of the Nonionized Valine Conformers

We present results of the first experimental observation of the nonionized natural amino acid valine. The study has employed the matrix-isolation IR spectroscopy and the density functional theory (DFT) and ab initio calculations. In the calculations geometries of nine low-energy valine conformers were optimized using the DFT method with the B3LYP parametrization and the 6-31++G** basis set. Additionally, the relative energies of the conformers were calculated at the MP2/6-31++G** level. The harmonic frequencies and IR intensities were calculated for all the minima found. These data were used to separate and assign the bands of the valine conformers in the experimental spectra. We found that two valine conformers are present in the Ar matrix: one with a bifurcated NH 2 ‚‚‚OdC H-bond (conformer Ia) and one with a N‚‚‚H-O H-bond (conformer IIa). A trace amount of a third valine conformer with NH 2 ‚‚‚O-C H-bond (conformer IIIb) was also detected. The conformational composition of the matrix-deposited nonionized valine was determined on the basis of observed and predicted IR intensities of the bands of different conformers. The composition is ∼94% of conformer Ia, ∼5% of conformer IIa, and less than 2% of conformer IIIb. The presence of three valine conformers in the Ar matrix results in broadening and/or in multiplex structures of some bands in the valine IR spectrum. Common features in the IR spectra of some nonionized natural aliphatic amino acids are discussed

Extrapolation of Multiplicity distribution in p+p(\bar(p)) collisions to LHC energies

The multiplicity (N_ch) and pseudorapidity distribution (dN_ch/d\eta) of primary charged particles in p+p collisions at Large Hadron Collider (LHC) energies of \sqrt(s) = 10 and 14 TeV are obtained from extrapolation of existing measurements at lower \sqrt(s). These distributions are then compared to calculations from PYTHIA and PHOJET models. The existing \sqrt(s) measurements are unable to distinguish between a logarithmic and power law dependence of the average charged particle multiplicity () on \sqrt(s), and their extrapolation to energies accessible at LHC give very different values. Assuming a reasonably good description of inclusive charged particle multiplicity distributions by Negative Binomial Distributions (NBD) at lower \sqrt(s) to hold for LHC energies, we observe that the logarithmic \sqrt(s) dependence of are favored by the models at midrapidity. The dN_ch/d\eta versus \eta for the existing measurements are found to be reasonably well described by a function with three parameters which accounts for the basic features of the distribution, height at midrapidity, central rapidity plateau and the higher rapidity fall-off. Extrapolation of these parameters as a function of \sqrt(s) is used to predict the pseudorapidity distributions of charged particles at LHC energies. dN_ch/d\eta calculations from PYTHIA and PHOJET models are found to be lower compared to those obtained from the extrapolated dN_ch/d\eta versus \eta distributions for a broad \eta range.Comment: 11 pages and 13 figures. Substantially revised and accepted for publication in Journal of Physics

Interleukin-32 in systemic sclerosis, a potential new biomarker for pulmonary arterial hypertension

Background: Pulmonary arterial hypertension (PAH) is a severe complication of systemic sclerosis (SSc), associated with a progressive elevation in pulmonary vascular resistance and subsequent right heart failure and death. Due to unspecific symptoms, the diagnosis of PAH is often delayed. On this basis, it is of great value to improve current diagnostic methods and develop new strategies for evaluating patients with suspected PAH. Interleukin-32 (IL-32) is a proinflammatory cytokine expressed in damaged vascular cells, and the present study aimed to assess if this cytokine could be a new biomarker of PAH during SSc. Methods: The IL-32 expression was evaluated in the sera and skin samples of 18 SSc-PAH patients, 21 SSc patients without PAH, 15 patients with idiopathic PAH (iPAH) and 14 healthy controls (HCs), by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC). Receiver-operating characteristic (ROC) curves were performed to evaluate the cut-off of IL-32 in identifying patients with PAH. Furthermore, in SSc patients, correlation analyses were performed between IL-32 sera levels and mean pulmonary artery pressure (mPAP) evaluated by right heart catheterization (RHC) and systolic pulmonary artery pressure (sPAP), obtained by echocardiography. Additionally, the number of skin IL-32+ cells was correlated with modified Rodnan skin score (mRSS). Results: In SSc-PAH patients, IL-32 sera levels were significantly higher when compared with SSc patients without PAH and patients affected by iPAH. The analysis of ROC curve showed that IL-32 sera levels above 11.12 pg/ml were able to predict patients with PAH (sensitivity = 90%, specificity = 100%). Furthermore, the IL-32 sera levels of patients with SSc correlated with both mPAP and sPAP. In the skin derived from SSc-PAH patients, the number of IL-32+ cells was significantly increased when compared with the skin derived from SSc patients without PAH, correlating with the mRSS. Conclusion: Our study suggested that sera determination of IL-32 may be a promising approach to evaluate the presence of PAH in SSc patients and together with longitudinal future studies could help to increase the understanding how these biomarkers mirror the vascular changes and the inflammatory process during SSc

Charged-Particle Multiplicity in Proton-Proton Collisions

This article summarizes and critically reviews measurements of charged-particle multiplicity distributions and pseudorapidity densities in p+p(pbar) collisions between sqrt(s) = 23.6 GeV and sqrt(s) = 1.8 TeV. Related theoretical concepts are briefly introduced. Moments of multiplicity distributions are presented as a function of sqrt(s). Feynman scaling, KNO scaling, as well as the description of multiplicity distributions with a single negative binomial distribution and with combinations of two or more negative binomial distributions are discussed. Moreover, similarities between the energy dependence of charged-particle multiplicities in p+p(pbar) and e+e- collisions are studied. Finally, various predictions for pseudorapidity densities, average multiplicities in full phase space, and multiplicity distributions of charged particles in p+p(pbar) collisions at the LHC energies of sqrt(s) = 7 TeV, 10 TeV, and 14 TeV are summarized and compared.Comment: Invited review for Journal of Physics G -- version 2: version after referee's comment

Matrix-Isolation Infrared and Theoretical Studies of the Glycine Conformers

The IR spectra of nonionized glycine and its deuterated derivatives isolated in the low-temperature argon matrices have been measured, and for the first time the infrared spectral characteristics of the three most stable conformers have been determined and assigned. Correlated level ab initio and density functional theory (DFT) calculations of IR frequencies and intensities with extended basis sets were performed and their results were employed to separate the bands of the glycine conformers in the experimental spectra and to assist the assignment. The intramolecular interconversion, conformer III w conformer I, which is observed in the matrices at temperatures higher than 13 K, was found to cause a significant decrease of the band intensities of conformer III in the spectra. This phenomenon was used to distinguish the vibrational bands of this conformer from the bands of the other conformers. The reliability of the Møller-Plesset second-order perturbation theory (MP2) method and the DFT method with the three-parameter density functional, Becke3LYP, in the prediction of the IR spectra of the nonionized glycine conformers was examined. We found that the DFT/Becke3LYP method with aug-cc-pVDZ basis set yields vibrational frequencies of the glycine conformers very similar to the MP2 results. Both DFT and MP2 results are in the excellent agreement with the experimental data

Blogging the Virtual: New Geographies of Domination and Resistance In and Beyond Russia

Russia’s accelerating authoritarian turn has not ignored the internet, and in recent years, the Russian state has clamped down on internet activities that diverge from the statist line, employing a variety of strategies to dominate online spaces. Nevertheless, oppositional voices flourish on the Russian internet, taking shape in independent blogs and videos. This paper explores three political bloggers through surveillant and resistance assemblages, making sense of this contestation through an interpretation of the Deleuzian virtual that underscores the emancipatory potential of online activities for producing more egalitarian configurations, but also taking stock of the ways that these technologies have increased domination. Encompassing the blurriness between digital and corporeal spaces, the paper contributes by revealing new geographies of contestation against state strategies to dominate the Russian internet. Overlapping with but not corresponding to Russian territorial boundaries, these dynamics highlight shifting spaces of power and resistance in the increasingly illiberal world

Mechanism of Disruption of the Amt-GlnK Complex by PII-Mediated Sensing of 2-Oxoglutarate

GlnK proteins regulate the active uptake of ammonium by Amt transport proteins by inserting their regulatory T-loops into the transport channels of the Amt trimer and physically blocking substrate passage. They sense the cellular nitrogen status through 2-oxoglutarate, and the energy level of the cell by binding both ATP and ADP with different affinities. The hyperthermophilic euryarchaeon Archaeoglobus fulgidus possesses three Amt proteins, each encoded in an operon with a GlnK ortholog. One of these proteins, GlnK2 was recently found to be incapable of binding 2-OG, and in order to understand the implications of this finding we conducted a detailed structural and functional analysis of a second GlnK protein from A. fulgidus, GlnK3. Contrary to Af-GlnK2 this protein was able to bind both ATP/2-OG and ADP to yield inactive and functional states, respectively. Due to the thermostable nature of the protein we could observe the exact positioning of the notoriously flexible T-loops and explain the binding behavior of GlnK proteins to their interaction partner, the Amt proteins. A thermodynamic analysis of these binding events using microcalorimetry evaluated by microstate modeling revealed significant differences in binding cooperativity compared to other characterized PII proteins, underlining the diversity and adaptability of this class of regulatory signaling proteins