Microbial Community Analysis of a Coastal Salt Marsh Affected by the Deepwater Horizon Oil Spill

Conceived and designed the experiments: MJB RJM BM PAS. Performed the experiments: MJB RJM SR JP YMP LMT JDVN. Analyzed the data: MJB RJM YMP LMT GLA TCH JDVN JZ PAS. Contributed reagents/materials/analysis tools: GLA TCH JZ BM PAS. Wrote the paper: MJB RJM PAS.Coastal salt marshes are highly sensitive wetland ecosystems that can sustain long-term impacts from anthropogenic events such as oil spills. In this study, we examined the microbial communities of a Gulf of Mexico coastal salt marsh during and after the influx of petroleum hydrocarbons following the Deepwater Horizon oil spill. Total hydrocarbon concentrations in salt marsh sediments were highest in June and July 2010 and decreased in September 2010. Coupled PhyloChip and GeoChip microarray analyses demonstrated that the microbial community structure and function of the extant salt marsh hydrocarbon-degrading microbial populations changed significantly during the study. The relative richness and abundance of phyla containing previously described hydrocarbon-degrading bacteria (Proteobacteria, Bacteroidetes, and Actinobacteria) increased in hydrocarbon-contaminated sediments and then decreased once hydrocarbons were below detection. Firmicutes, however, continued to increase in relative richness and abundance after hydrocarbon concentrations were below detection. Functional genes involved in hydrocarbon degradation were enriched in hydrocarbon-contaminated sediments then declined significantly (p<0.05) once hydrocarbon concentrations decreased. A greater decrease in hydrocarbon concentrations among marsh grass sediments compared to inlet sediments (lacking marsh grass) suggests that the marsh rhizosphere microbial communities could also be contributing to hydrocarbon degradation. The results of this study provide a comprehensive view of microbial community structural and functional dynamics within perturbed salt marsh ecosystems.Yeshttp://www.plosone.org/static/editorial#pee

Surgery for emphysema

[No abstract available

Repertorio delle continuazioni italiane ai romanzi cavallereschi spagnoli. Ciclo di Amadis di Gaula

Repertorio delle continuazioni italiane al ciclo di Amadis di Gaula con studio introduttivo, censimento bibliografico, sommari, indice dei personaggi. Di Anna Bognolo le parti seguenti: Premessa pp. 11-24Introduzione: Cap. 1 - Mambrino Roseo da Fabriano: vita provvisoria di uno scrittore pp. 25-76Cap. 4 - Il ciclo italiano di Amadis di Gaula pp. 149-161Repertorio: Cap. 1 - Sommari pp. 261-270.Di Giovanni Cara le parti seguenti: Repertorio: Cap. 1 - Sommari pp. 271-380.Di Stefano Neri le parti seguenti: Introduzione: Cap. 2 - Libros de caballerias pp. 77-84Cap. 3 - Il romanzo cavalleresco spagnolo in Italia pp. 85-140Cap. 4 - Il ciclo italiano di Amadis di Gaula pp. 141-148 e 162-176Cap. 5 - Tabelle pp. 177-198Cap. 6 - Censimento bibliografico del ciclo italiano di Amadis di Gaula pp. 199-260Repertorio: Cap. 1 - Sommari pp. 381-508Cap. 2 - Indice dei personaggi pp. 509-66

Early coronary artery bypass graft thrombosis in a patient with protein S deficiency

A 59-year-old white mall underwent multiple coronary artery bypass grafts (CABGs) on an emergency basis for severe stenosis of the left main, and occlusion of the right coronary, artery, One month after operation recurrence of angina prompted a new diagnostic evaluation, Occlusion of the grafts was detect ecl at angiography. Accurate hematological screening before reoperation showed decreased levels of protein S. One year after his second operation, the patient is asymptomatic on oral anticoagulant therapy. Tallium-201 scintigraphy shows normal myocardial perfusion

Early coronary artery bypass graft thrombosis in a patient with protein S deficiency

A 59-year-old white mall underwent multiple coronary artery bypass grafts (CABGs) on an emergency basis for severe stenosis of the left main, and occlusion of the right coronary, artery, One month after operation recurrence of angina prompted a new diagnostic evaluation, Occlusion of the grafts was detect ecl at angiography. Accurate hematological screening before reoperation showed decreased levels of protein S. One year after his second operation, the patient is asymptomatic on oral anticoagulant therapy. Tallium-201 scintigraphy shows normal myocardial perfusion

PROTECTION AND REVASCULARIZATION OF BRONCHIAL ANASTOMOSES BY THE INTERCOSTAL PEDICLE FLAP

We used an improved method for preparation of the intercostal pedicle flap for encircling bronchial anastomoses, and we studied its vascular supply after the operation. The flap was used in 56 patients undergoing various types of sleeve resection and in three patients undergoing single lung transplantation. The technique is simple, fast, and causes neither extra surgical trauma nor complications. It allows satisfactory isolation and sealing of the bronchial anastomosis. Even if complete anastomotic dehiscence occurs (one case), the flap preserves the continuity of the airway, thus avoiding bronchopleural fistulas or other complications. The postoperative arteriographic study of the intercostal artery supplying the flap (performed in 14 patients) demonstrated the full patency of the vessel in all cases. It also showed that a fine vascular network develops around the anastomosis early in the postoperative period