154 research outputs found
Specific heats of dilute neon inside long single-walled carbon nanotube and related problems
An elegant formula for coordinates of carbon atoms in a unit cell of a
single-walled nanotube (SWNT) is presented and the potential of neon (Ne)
inside an infinitely long SWNT is analytically derived out under the condition
of the Lennard-Jones potential between Ne and carbon atoms.
Specific heats of dilute Ne inside long (20, 20) SWNT are calculated at
different temperatures. It is found that Ne exhibits 3-dimensional (3D) gas
behavior at high temperature but behaves as 2D gas at low temperature.
Especially, at ultra low temperature, Ne inside (20, 20) nanotubes behaves as
lattice gas. A coarse method to determine the characteristic temperature
for low density gas in a potential is put forward. If
, we just need to use the classical statistical
mechanics without solving the Shr\"{o}dinger equation to consider the thermal
behavior of gas in the potential. But if , we
must solve the Shr\"{o}dinger equation. For Ne in (20,20) nanotube, we obtain
K.Comment: 14 pages, 7 figure
Cell-mediated exon skipping normalizes dystrophin expression and muscle function in a new mouse model of Duchenne Muscular Dystrophy
Cell therapy for muscular dystrophy has met with limited success, mainly due to the poor engraftment of donor cells, especially in fibrotic muscle at an advanced stage of the disease. We developed a cell-mediated exon skipping that exploits the multinucleated nature of myofibers to achieve cross-correction of resident, dystrophic nuclei by the U7 small nuclear RNA engineered to skip exon 51 of the dystrophin gene. We observed that co-culture of genetically corrected human DMD myogenic cells (but not of WT cells) with their dystrophic counterparts at a ratio of either 1:10 or 1:30 leads to dystrophin production at a level several folds higher than what predicted by simple dilution. This is due to diffusion of U7 snRNA to neighbouring dystrophic resident nuclei. When transplanted into NSG-mdx-Î51mice carrying a mutation of exon 51, genetically corrected human myogenic cells produce dystrophin at much higher level than WT cells, well in the therapeutic range, and lead to force recovery even with an engraftment of only 3-5%. This level of dystrophin production is an important step towards clinical efficacy for cell therapy
1H NMR-based metabolomics combined with HPLC-PDA-MS-SPE-NMR for investigation of standardized Ginkgo biloba preparations
Commercial preparations of Ginkgo biloba are very complex mixtures prepared from raw leaf extracts by a series of extraction and prepurification steps. The pharmacological activity is attributed to a number of flavonoid glycosides and unique terpene trilactones (TTLs), with largely uncharacterized pharmacological profiles on targets involved in neurological disorders. It is therefore important to complement existing targeted analytical methods for analysis of Ginkgo biloba preparations with alternative technology platforms for their comprehensive and global characterization. In this work, 1H NMR-based metabolomics and hyphenation of high-performance liquid chromatography, photo-diode array detection, mass spectrometry, solid-phase extraction, and nuclear magnetic resonance spectroscopy (HPLC-PDA-MS-SPE-NMR) were used for investigation of 16 commercially available preparations of Ginkgo biloba. The standardized extracts originated from Denmark, Italy, Sweden, and United Kingdom, and the results show that 1H NMR spectra allow simultaneous assessment of the content as well as identity of flavonoid glycosides and TTLs based on a very simple sample-preparation procedure consisting of extraction, evaporation and reconstitution in acetone-d6. Unexpected or unwanted extract constituents were also easily identified in the 1H NMR spectra, which contrasts traditional methods that depend on UV absorption or MS ionizability and usually require availability of reference standards. Automated integration of 1H NMR spectral segments (buckets or bins of 0.02Â ppm width) provides relative distribution plots of TTLs based on their H-12 resonances. The present study shows that 1H NMR-based metabolomics is an attractive method for non-selective and comprehensive analysis of Ginkgo extracts
Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: Comparisons with the normal articular cartilage
<p>Abstract</p> <p>Background</p> <p>We have recently found a phenomenon that spontaneous regeneration of a hyaline cartilage-like tissue can be induced in a large osteochondral defect by implanting a double-network (DN) hydrogel plug, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N, N'-Dimetyl acrylamide), at the bottom of the defect. The purpose of this study was to clarify gene expression profile of the regenerated tissue in comparison with that of the normal articular cartilage.</p> <p>Methods</p> <p>We created a cylindrical osteochondral defect in the rabbit femoral grooves. Then, we implanted the DN gel plug at the bottom of the defect. At 2 and 4 weeks after surgery, the regenerated tissue was analyzed using DNA microarray and immunohistochemical examinations.</p> <p>Results</p> <p>The gene expression profiles of the regenerated tissues were macroscopically similar to the normal cartilage, but showed some minor differences. The expression degree of COL2A1, COL1A2, COL10A1, DCN, FMOD, SPARC, FLOD2, CHAD, CTGF, and COMP genes was greater in the regenerated tissue than in the normal cartilage. The top 30 genes that expressed 5 times or more in the regenerated tissue as compared with the normal cartilage included type-2 collagen, type-10 collagen, FN, vimentin, COMP, EF1alpha, TFCP2, and GAPDH genes.</p> <p>Conclusions</p> <p>The tissue regenerated by using the DN gel was genetically similar but not completely identical to articular cartilage. The genetic data shown in this study are useful for future studies to identify specific genes involved in spontaneous cartilage regeneration.</p
Estrogen regulation of apoptosis: how can one hormone stimulate and inhibit?
The link between estrogen and the development and proliferation of breast cancer is well documented. Estrogen stimulates growth and inhibits apoptosis through estrogen receptor-mediated mechanisms in many cell types. Interestingly, there is strong evidence that estrogen induces apoptosis in breast cancer and other cell types. Forty years ago, before the development of tamoxifen, high-dose estrogen was used to induce tumor regression of hormone-dependent breast cancer in post-menopausal women. While the mechanisms by which estrogen induces apoptosis were not completely known, recent evidence from our laboratory and others demonstrates the involvement of the extrinsic (Fas/FasL) and the intrinsic (mitochondria) pathways in this process. We discuss the different apoptotic signaling pathways involved in E2 (17ÎČ-estradiol)-induced apoptosis, including the intrinsic and extrinsic apoptosis pathways, the NF-ÎșB (nuclear factor-kappa-B)-mediated survival pathway as well as the PI3K (phosphoinositide 3-kinase)/Akt signaling pathway. Breast cancer cells can also be sensitized to estrogen-induced apoptosis through suppression of glutathione by BSO (L-buthionine sulfoximine). This finding has implications for the control of breast cancer with low-dose estrogen and other targeted therapeutic drugs
Spectroscopic study of pentacene assembled in Langmuir-Blodgett film mixed with stearic acid
12-16<span style="font-size:
15.5pt;mso-bidi-font-size:8.5pt;font-family:" times="" new="" roman","serif""="">Langmuir
Blodgett (LB) films of non-amphiphilic pentacene mixed with stearic acid have
been prepared and its photo-physical properties are reported here. Surface
pressure versus area-per-molecule isotherms (Ï-A) at different compositions are
measured. Spectroscopic properties (UV-Visible absorption, emission and Scanning
Electron Micrograph) of pentacene in LB film have been reported. The
blue-shifted absorption and red shifted emission suggest the formation of H-type
of aggregates.
</span
Genesis of Enhanced Raman Bands in SERS Spectra of 2âMercaptoimidazole: FTIR, Raman, DFT, and SERS
The surface enhanced Raman scattering (SERS) spectra
of biologically
and industrially significant, 2-mercaptoimidazole (2-MI) molecule
have been investigated. The SERS spectra of the molecule at different
concentrations of the adsorbate are compared with its Fourier transform
infrared (FTIR) and normal Raman spectra (NRS) in varied environments.
The optimized molecular structures and vibrational wavenumbers of
the various forms (ca. cationic, neutral, ylidic, anionic) of the
molecule have been estimated from the density functional theory (DFT).
The vibrational signatures of the molecule have been assigned for
the first time from the potential energy distributions (PEDs). The
analyses of the Raman vibrational signatures reveal the coexistence
of all the different forms of the molecule in the solid state and
in aqueous solution. Concentration dependent SERS spectra of the molecule
at neutral pH of the medium together with the multivariate data analyses
techniques also suggest the concomitance of all the probable forms
of the molecule in the surface adsorbed state. The genesis of selective
enhancements of the Raman bands in the SERS spectra emanating from
the cationic, neutral, ylidic and anionic forms of the molecule have
been divulged from the view of the Albretchtâs âAâ
and HerzbergâTeller (HT) charge transfer (CT) contribution
Estimation of improvement in elastic moduli for functionalised defective graphene-based thermoplastic polyurethane nanocomposites: a molecular dynamics approach
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