Isolation, Screening and Characterization of Exopolysaccharide Producing Pseudomonas Sp. from The Soil Sample

  Exopolysaccharides (EPS) are vital metabolites that specific environmental microorganisms produce and expel. The goal of the current investigation is to identify bacteria that produce exopolysaccharides in soil samples from the Buttenath Dam region, At. Chanai, Tq. Ambajogai, Dist. Beed situated in the Marathwada region of Maharashtra, India. Two methods, such as gravimetric analysis of exopolysaccharide dry weight and quantification as, instance, for total carbohydrate content using phenol sulphuric acid technique, were used to screen the EPS-producing capacities of the chosen isolates. Post preliminary screening by selecting thick, ropy-like colony formers on agar medium, the results showed that thirteen (13) distinct colonies were marked to manufacture EPS. Tentative identification of these isolates was done based on morphological and biochemical tests. Three (03) isolates, VJ001, VJ003 and VJ010, were identified through secondary screening as the most effective EPS producers (producing precipitates over 1.46 mg/L of total dry weight and 8). This was in comparison to other bacterial colonies that were also isolated. Out of these screened isolates, VJ003 was the isolate with the highest exopolysaccharide produced and this isolated bacterium was identified using 16S rRNA gene sequence and phylogeny study as Pseudomonas aeruginosa VJ003 with NCBI GeneBank accession no. OR196093. The Pseudomonas aeruginosa VJ003 strain can be used in biotechnological sectors because of the ability it has to create significant levels of exopolysaccharides, according to the results of the current study

Prospective study comparing early functional outcome and gait analysis in femoral neck fracture treated by cemented hemiarthroplasty using modified Hardinge approach and conventional posterior approach

Background: A prospective study was done to compare the outcomes of management of fracture neck of femur by cemented hemiarthroplasty using modified Hardinge approach and conventional posterior approachMethods: The 2019 to 2022 50 patients underwent bipolar hemiarthroplasty for femoral neck fracture. Group A (Femoral neck fracture treated using conventional posterior approach) and group B (Femoral neck fracture treated using modified Hardinge approach) with 25 patients in each group. Outcomes were evaluated based on mean surgical time and Harris hip score and SF-36.Results: Mean duration of surgery in minutes was more for modified Hardinge approach. Harris hip score with standard deviation for modified Hardinge Approach for follow-ups was better and statistically significant than posterior approach. Similarly, quality of life after surgery, in terms of mean SF-36 score with standard deviation for modified Hardinge approach was better and statistically significant than posterior approach. Modified Hardinge approach has fewer complications in comparison to the posterior approach. With the advantages comes a longer learning curve to operate without complications. Hence, with proper surgical technique, and proper tight closure, we prefer the modified Hardinge approach over other approaches as it had nil dislocations and abductor lurch.Conclusions: Modified Hardinge approach for hip arthroplasty in elderly people with femoral neck fracture provide significant benefit in the early post operative period when compared to conventional posterior approach in terms of post operative pain, time of recovery, dislocation rate and quality of life

Prospective study of management of unstable intertrochantric femur fractures in elderly: hemiarthroplasty vs. osteosynthesis by proximal femoral nail

Background: A prospective study was done to compare the outcomes of management of unstable intertrochantric femur fractures in elderly by hemiarthroplasty and osteosynthesis by proximal femoral nail.Methods: This was an interventional prospective study. The study was carried out over 3 years from 2019 to 2022 at PCMC’s PGI YCMH, Pimpri, Pune, Maharashtra, India. The study was carried out over 3 years from 2019 to 2022;50 Patients were randomly divided into two groups; Group A (unstable intertrochanteric femur fracture patients treated by bipolar hemi-arthroplasty) and Group B (unstable intertrochanteric femur fracture patients treated by proximal femoral nailing) with 25 patients in each group. Outcomes were evaluated based on functional outcomes, mean surgical time, mean blood loss during surgery, post-operative length of stay, post-operative complications. Harris hip Score was used to assess clinical functional outcomes.Results: The PFN group showed a better Harris Hip score at 1, 3, and 6 months follow ups. Mean surgical time was more in bipolar hemiarthroplasty group. Mean blood loss during surgery was more in bipolar hemiarthroplasty group. Post-operative length of stay was more in bipolar hemiarthroplasty group. Post-operative complications like infections, limb length discrepencies were more in bipolar hemiarthroplasty group, whereas 2 implant failures occurred in PFN group compared to a single one in bipolar hemiarthroplasty group.Conclusions: Both PFN and bipolar-hemiarthroplasty appear to produce satisfactory outcomes in surgically treated unstable IT fractures, bipolar-hemiarthroplasty has the advantages of early mobilization and early weight bearing, no risk of non-union but PFN group is superior when it comes to functional outcomes, mean surgical time, Mean blood loss during surgery, post-operative length of stay, post-operative complications

ELECTRICAL ENERGY CONSERVATION IN A DISTILLERY PLANT- A CASE STUDY

Energy is a main function for each kind of process. It is the central force behind our productivity, our leisure and our environment. Energy Audit is the integral part of Energy Management. The energy audit can unearth huge profits to the industry. It quantifies the energy uses according to its various functions. It attempts to balance the total energy inputs with the output or the uses. The energy conservation and maximization strategies for a process industry like distillery plant are cost effective, which conserve the environment automatically. The electrical energy audit of a distillery plant has wide scope of energy conservation. The audit has been successfully completed and concluded with the saving of the 18500 kWh per year of energy. The most of the electrical energy is utilized to drive electrical motors used for various processes. Energy will be saving in case of motors with the help of the variable frequency drives, which reduces the speed of the motors as well as energy. The distillery has the beneficial of Rs. 1, 20,910 by implementing given recommendations

Site Catchment Analysis of Mahalena Cave of Rajagala, Sri Lanka: With Special Reference to the Prehistoric and Anuradhapura Phase

The systematic archaeological survey was carried out around the 0-10 km radius of Mahalena cave site. Documentation of natural resources available within the estimated radius. A few satellite settlements or supportive settlements were identified within the radius of Mahalena cave. The chief aim of this research is to understand the suitability of the landscape and natural resources available within the vicinity of this archaeological site. Mahalena cave site was subjected to large-scale excavation for several seasons by Sri Lankan and Indian archaeologists. A Few seasons of detailed excavations have provided us with sufficient data to study the resource exploitation pattern around the Mahalena cave. The study of the resource exploitation pattern or site catchment study is one of the important tools to reconstruct the economy of ancient settlers of any particular region. Resources lying within the economic range of individual archaeological sites support ancient inhabitants for their day-to-day living. The current research will be helpful in identifying suitable factors which lead the Prehistoric and Early Historic inhabitants of the Mahalena cave to choose this particular location for their settlement.  DOI: http://doi.org/10.31357/fhss/vjhss.v08i02.1

Aryl esters and arylamides of 2-hydroxy-3-naphthoic acid and 1-bromo-2-hydroxy-3-naphthoic acid

This article does not have an abstract

Oceanographic parameters and their relationship to fish catch estimation: a case study in coastal waters north of Cochin during 1981

Coastal waters in the North of Cochin was selected for the present study. This area is well known for the occurrence and abundance of fish schools of oil sardine (Sardinella Iongiceps) and mackerel (Rastrelliger Kanagurta) allowing the period after SW monsoon in the Indian Ocea

Remote sensing of ocean colour and targeting of fish schools from airborne sensors

Studying the well known pelagic school of oil sardine and mackerel in the Arabian sea of the Indian Ocean. A high productivity is reported in the oceanic waters of Cochin preceding SW monsoon. A remote sensing experiment was carried out in the oceanic waters over North of Cochin coast involving vessel-based sea truth data collection synchronous to aircraft overflights during October, November and December 1981

Implementation of Decoy Deception based Detection System for Ransomware Attack

Ransomware poses a dangerous threat to  cybersecurity. Data as well as rights owned by the user are  adversely impacted. The situation has become considerably  more critical as a result of the emergence of new ransomware  varieties and Ransomware-as-a-Service. In this paper, we  presented a novel deception-based and behaviour-based  method for real-time ransomware detection. In order to avoid  any loss before ransomware is discovered, we build pretend  files and directories for nefarious behaviours. We conducted a  pilot study using Locky, and the results demonstrate the  effectiveness of our strategy with little system resource usage  and geographical cost.&nbsp

Callosbruchus maculatus Resistance in Some Wild Relatives and Interspecific Derivatives of Pigeonpea

The present study of Screening of some wild relatives of pigeonpea against bruchid revealed that amongst the 4 Cajanus species, comprising of seven accessions, bruchid damage ranged between 14 and 30%, compared to 78% in the commercially cultivated pigeonpea variety ICPL 85010. Wild species Cajanus scarabaeoides accession ICPW 130 and C. platycarpus accession ICPW 66 had lowest damage (14% and 16% respectively). The larval/pupal period was prolonged in wild accessions (42 – 55days) compared to 33 days on the susceptible control ICPL 85010 indicating the antibiosis mechanism of resistance in the wild species. Though there was high oviposition on the seeds of interspecific derivative of C. platycarpus A 4-10-7-19, it had the least damage (10%). In the rest of the derivatives the damage rating ranged between 10 and 55% compared to 80% damage in the susceptible control, reflecting the potential of utilizing these wild species derivatives in pigeonpea crop improvement to overcome the Bruchid damage