Effects of housing system on welfare and milk yield and quality of Girgentana goats

Effetti del sistema di stabulazione sul benessere e sulla produzione di latte di capre Girgentane \u2013 La stabulazione in poste individuali, in uso per la razza Girgentana, per evitare che le capre possano procurarsi lesioni con le corna, viene confrontata con la stabulazione libera, valutando il grado di benessere e la produttivit\ue0 di 40 capre. Le Girgentane tenute alla posta hanno fatto rilevare buone condizioni di benessere e un pi\uf9 elevato livello produttivo, collegati alla mancanza degli effetti stressanti della gerarchia sociale ed alla possibilit\ue0 dell\u2019integrazione individuale. Tuttavia, assicurando adeguata igiene e sufficiente spazio, le Girgentane hanno mostrato di poter adattarsi anche alla stabulazione libera, che ha offerto maggiori possibilit\ue0 di movimento e relazioni sociali, e ha indotto una migliore risposta immunitaria; la minore esigenza di manodopera compenserebbe il pi\uf9 basso livello produttivo registrato con tale sistema.In the past years, in Sicily, goats of the Girgentana breed have decreased so drastically in number that they risked extinction. Among the causes, particular blame can be laid at the traditional housing system, in which the goats, after coming back from pasture, were tethered in individual wooden stalls. Such a system is still considered necessary by breeders in order to avoid aggressive and harmful behaviour on the part of the animals, due to the presence of their long horns (Giaccone et al., 1994). This belief negatively interferes with the action recently initiated for the recovery and regeneration of the breed (Portolano et al., 2002); in fact, the tie stalls housing, increasing manpower, limits the increase in size in herds of Girgentana goats. On the other hand, the biological production method does not admit the tie stall housing, but imposes a housing system that ensures that animals have freedom of movements and can express normal social behaviour. The present study aims to contribute to the development of the Girgentana breed, evaluating its adaptation to free-housing. Goats tethered in stalls and free-housed in a straw-bedded pen were compared by assessing their behavioural, immunological, endocrine and productive responses

Fatty acid composition of salami made by meat from different commercial categories of indigenous dairy cattle

In autochthonous dairy cattle farms, the production of salami could represent an alternative commercial opportunity. Therefore, a study was carried out to investigate the fatty acid (FA) composition of salami made using the meat from grazing (GB) or housed (HB) young bulls and grazing adult cows (AC) of Cinisara breed. The products were manufactured by adding 20% of pork lard. Animal category influenced the FA composition, although the addition of lard mitigated the differences found in fresh meat. The salami from GB showed higher polyunsaturated FA content (p ≤0.01) and, in particular, a higher level of linoleic acid (p ≤0.05), than from other animal categories. Salami made from AC meat showed lower polyunsaturated/saturated FA ratio (p ≤0.05), but a better n-6/n-3 ratio compared to HB (p ≤0.05), due to the lower content of linoleic acid. Multivariate analysis showed an important influence of animal category on FA composition due to age, feeding system and meat fat content of animals, despite the addition of lard

Effects of Grazing Season on Physico-Chemical Characteristics and Fatty Acids of Nutritional Interest of Caciocavallo Palermitano Cheese

The aim of this work was to evaluate, in the different production seasons of the year, the physico-chemical quality of an artisanal cheese traditionally obtained from autochthonous grazing cows, with particular reference to fatty acids (FA) of nutritional interest that play an important role in the risk or prevention of some human pathologies. For this purpose, cheeses were sampled in 11 farms, repeating the samplings in 3 different periods of the year (summer, autumn–winter, and spring) when the productive conditions of the pastures varied. The cheeses produced in the spring period, when cows ingest a greater amount of grazed forage, resulted in a more adequate composition of the main FA, which are recognized as having a health effect, such as α-linolenic, trans-vaccenic, rumenic, docosapentaenoic (DPA) and docosahexaenoic (DHA) acids. Branched-chain FA were found in greater quantities in spring cheeses, as well as in summer ones. The FA composition of cheeses produced in the different seasons was reflected in some nutritional indexes that also resulted as more suitable in cheeses obtained in the spring period. The positive effects induced on the FA profile of cheeses are presumably linked to the diet of autochthonous cows, which is mainly based on forage from natural pastures. Therefore, the results obtained confirm the benefits of grazing, which is able to guarantee the production of healthier cheeses for consumers

Milk production and physiological traits of ewes and goats housed indoor or grazing at different daily timing in summer

During a 6 week trial in summer, 3 homogeneous groups, each consisting of 5 Comisana ewes and 5 Rossa Mediterranea goats, grazed watered forage resources during day (D) or night (N), or were housed indoor and supplied with mowed herbage(H). Maximum THI peaked at 94 at the end of July, and was almost constantly higher of 80. Milk yield was higher in N than in D and H goats, whereas N ewes produced more milk than H group, but their milk yield was higher than D ewes only in the period with the highest THI values. The lower urea in N goat milk, and the higher casein in N ewe milk, seem to indicate a better efficiency in dietary nitrogen utilization of night grazing animals. N ewes showed lower SCC in milk, and higher incidence of clotting milk samples, in comparison to other ewes. N goats and ewes showed lower rectal temperature and pulse rate in the afternoon and, among metabolic parameters, higher hematic level of sodium. Night grazing was confirmed to be a management practice for increasing heat tolerance, to which goats appeared to be more sensitive in terms of milk yield

Fatty acids as biomarkers of the production season of caciocavallo palermitano cheese

This experiment aims to evaluate the potential of fatty acids (FA) of Caciocavallo Palermitano cheese as biomarkers of production season and pasture-based diet. A total of 48 cheeses were made in the four seasons with milk from two farms that raised cows of Cinisara breed. The animals were fed on pasture with supplementation of wheat bran and wheat straw in the barn, and in summer also with Opuntia ficus-indica cladodes. The chemical composition and FA profile of cheese were influenced by the season and not by the farm. In particular, cheeses produced in spring were characterized by higher protein and lower fat, and showed higher contents in trans-vaccenic acid, α-linolenic acid, rumenic acid, n-3 polyunsaturated FA (PUFA), and total PUFA. In winter, the lower availability of grazing forage, requiring a higher level of feeding integration, was responsible for an increase of saturated FA (SFA). The multivariate analysis distinguished clearly the cheeses made in winter and spring, while those produced in autumn and summer showed some overlapping points. Further investigations should be carried out to evaluate the effects of type and level of feeding integration on the presence of FA more suitable to be used as biomarkers of period and diet

Effects of feeding green forage of sulla (Hedysarum coronarium L.) on lamb growth and carcass and meat quality.

The nutritional effects of sulla (Hedysarum coronarium L.) forage containing condensed tannins (CT) on growth of lambs, and carcass and meat quality were investigated. Thirty-two male Comisana lambs aged 100 ± 8 days weighing 19.0 ± 2.8 kg were fed fresh forage of sulla or CT-free annual ryegrass (Lolium multiflorum Lam. subsp. Wersterwoldicum) for 49 days until slaughter; in addition, each lamb was supplied with 200 g/days of concentrate. Eight lambs per diet had been previously treated with anthelmintic drugs to remove nematode parasites. Measurements of BW and feed intake, and counts of faecal nematode eggs were made. Carcass parameters were recorded after slaughter, and tissue components of the hind leg were determined. Longissimus dorsi meat was evaluated for pH, colour, thawing and cooking losses, Warner-Bratzler shear force, chemical composition and sensory properties based on triangle tests. Relative to ryegrass-fed lambs, sulla-fed lambs had significantly greater dry matter (DM) and protein intake, a more favourable feed conversion ratio, and superior growth rate, final BW at 150 days of age, carcass weight, yield and fatness. These results were attributed to the high protein and non-structural carbohydrate content of sulla, and also to the moderate CT content of sulla (16.7 and 20.3 g/kg of DM in offered and consumed sulla forage, respectively). Anthelmintic treatment did not affect lamb growth, as the level of parasitic infection (initial and final) was low. The physical, chemical and sensory properties of the lamb meat were not influenced by diet

Effects of short- and long-term fasting on the ovarian axis and reproductive performance of rabbits does

To assess the impact of acute nutritional challenges on reproductive performance, 116 rabbit does were randomly assigned to either \u201ccontrol\u201d or two \u201cfasting\u201d groups. In the control group, the does were fed ad libitum (AL) while in the treated groups the animals were fasted for 24 (STF) or 48 h (LTF) before artificial insemination (AI). In both treated groups, the rabbits were re-feed 2 hours before AI, which was performed at day 11 post partum (pp) in STF does and at day 32 or 55 pp in LTF does following GnRH injection to induce ovulation. In a parallel experiment, blood samples were collected from 10 rabbits/groups by catheterization of the central ear vein at 15 min intervals for 4 h prior and after GnRH, then at hourly intervals for other 4 h, and again 24 and 48 h later to measure LH, estradiol-17b? and leptin. The expression of estradiol-17b receptors (ER) was evaluated by immunohistochemistry in the hypothalamus and anterior pituitary obtained from both control and treated rabbits after 24 and 48 h of fasting. The 24-h long fasting reduced receptivity (55.8 vs. 70.9%), fertility rate (42.8 vs. 59.2%) especially in does with parity =4 (50 vs. 80%; P=0.05), and kits born alive (6.6 vs. 7.7). Also the does fasted for 48 h, showed lower fertility (31.2 vs. 52,6 %) and litter size at birth (8.7 vs. 9.6) than controls. Following GnRH injection, the LH peak was higher in AL and STF than in LTF does (Mean \ub1 SEM, 39.3\ub117.04 vs. 31.3\ub13.39 and 12.2\ub11.23 ng/ml, respectively). Estradiol-17b showed higher pulse frequency and amplitude in AL than in STF and LTF does. Toward the end of fasting, mean plasma leptin concentrations were lower (P\ua30.01) in both STF and LTF does than in control rabbits (0.82\ub10.19, 1.20\ub10.1, and 2.32\ub10.33 ng/ml, respectively), but then gradually increased in both treated groups following re-alimentation. Compared to controls or STF does, ER-like immunoreactivity decreased in the ventromedial nucleus of LTF rabbits, but increased in the medial preoptic area. ER expression was greatly reduced in the anterior pituitary of LTF does

Effect of the inclusion of fresh lemon pulp in the diet of lactating ewes on the properties of milk and cheese

This study investigated the effects of fresh lemon pulp (FLP), as a natural antioxidant in the diet, on the intake of feed and the production of milk and cheese of Valle del Belice lactating ewes during the hot summer in Sicily. A total of 15 second-lambing ewes, kept individually in 3  7 3 m pens, were divided into 3 homogeneous groups fed with 3 diets in a 3  7 3 Latin square design, with 3 experimental phases of 21 days each. The diets were: mixed hay ad libitum plus 600 g/day of concentrate (FLP0); mixed hay ad libitum plus 400 g/day of concentrate and 1 kg/day of FLP (FLP1); and mixed hay ad libitum plus 200 g/day of concentrate and 2 kg/day of FLP (FLP2). Nine experimental Pecorino cheeses were manufactured with bulk milk collected at the end of each phase from each group. The ewes of each group showed the same dry matter (DM) intake (2 kg/day/head), but the FLP2 group received lower (P = 0.001) net energy for lactation (NEL) than other groups (2.13, 2.36, and 2.31 Mcal/day per head for FLP2, FLP0, and FLP1, respectively). The FLP constituted 9% and 16% of the total DM intake in the FLP1 and FLP2 groups, respectively. In general, the daily milk yield was low, reflecting the effect of the high environmental temperatures, and was lower (P = 0.001) in the FLP2 group than in the other groups (323, 355, and 369 g/day for FLP2, FLP1, and FLP0, respectively), probably due to the lower daily energy intake. Milk protein (P = 0.046) and casein (P = 0.033) percentages were higher in the FLP2 group than in the FLP1 group; the FLP-fed groups had higher levels of (P = 0.011) milk urea than the FLP0 group, due to a higher (P = 0.001) CP/NELratio in the ingested diet (96.4, 95.8, and 95.3 g/Mcal for FLP2, FLP1, and FLP0, respectively). The fatty acid composition of milk from FLP2-fed ewes was higher in vaccenic (10.6 vs. 7.96 mg/g fat; P = 0.031) and rumenic acids (6.21 vs. 5.30 mg/g fat; P = 0.048) than that in milk from FLP0 ewes. The characteristics of the cheeses were not influenced by the diet, with the exception of the total content of phenolic compounds (P = 0.011) and antioxidant activity (P = 0.051), both of which were higher in cheeses made with milk from FLP-fed ewes

Neuroendocrine responses in neonatal mother-deprived rabbits

To study both short- and long-term adaptation mechanisms activated by rabbits which were separated from their mothers (DLS) for 48 h between postnatal days 9 and 11, we examined plasma corticosterone concentrations before, during, and after DLS as well as the expression of glucocorticoid receptor (GR) in the hippocampus and the adrenal axis responses to a mild stress stimuli or exogenous ACTH injection. At postnatal day 10, plasma corticosterone concentration of DLS rabbits was similar to that of controls, but rose two-fold at day 11 (17.7 ± 1.3 vs. 9.3 ± 1.2 μg/dl, P < 0.01) and then decreased at day 12, when suckling was allowed again, to match those of controls with no difference thereafter. At postnatal day 14, both control and DLS rabbits had similar basal corticosterone concentrations (2.2 ± 0.4 vs. 2.3 ± 1.1 μg/dl, respectively) as well as at day 120 (8.8 ± 3.2 vs. 9.7 ± 2.8 μg/dl, respectively). After the standardized stress stimulus, plasma corticosterone concentrations were lower in DLS rabbits than controls at postnatal days 14 (P < 0.01) and 120 (P < 0.05). At day 120, corticosterone levels rose similarly seven-fold (P < 0.01) within 30 min after ACTH administration and remained sustained thereafter in both control and DLS rabbits. Positive immunoreactivity for GR was detected in the hippocampus and in the dorsal medial hypothalamic region at postnatal day 14. The present data suggest that 48 h DLS from postnatal days 9 to 11 results in a modified hypothalamic–pituitary–adrenal axis reactivity later in life