Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novel ornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl(-1) with NAA ( 0.0, 0.5, 1.0 mgl(-1)) and BA (0.0, 0.5, 1.0, 2.0 mgl(-1)) with IAA ( 0.0, 0.5, 1.0, mgl(-1)) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl(-1) BA and 1.0 mgl(-1) NAA (70%) followed by 1.0 mgl(-1) BA and 0.5 mgl(-1) NAA (50%), 1.0 mgl(-1) BA and 1.0 mgl(-1) NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl(-1) BA and 0.5 mgl(-1) IAA (43%) followed by 0.5 mgl(-1) BA and 1.0 mgl(-1) IAA (23%)

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novelornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-¹ with NAA (0.0, 0.5, 1.0 mgl-¹) and BA (0.0, 0.5, 1.0, 2.0 mgl-¹) with IAA (0.0, 0.5, 1.0, mgl-¹) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-¹ BA and 1.0 mgl-¹ NAA (70%) followed by 1.0 mgl-¹ BA and 0.5 mgl-¹ NAA (50%), 1.0 mgl-¹ BA and 1.0 mgl-¹ NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-¹ BA and 0.5 mgl-¹ IAA (43%) followed by 0.5 mgl-¹ BA and 1.0 mgl-¹ IAA (23%)

Soluble CD40 ligand and prolactin levels in migraine patients during interictal period

The relationship of migraine with cardiovascular diseases has been clarified by many studies, and currently, migraine is suggested to be a systematic vasculopathy. Inflammation, thrombosis and impaired vascular reactivity are the underlying pathophysiological mechanisms of the vasculopathy. In the present study, we aimed to investigate the relationship between prolactin levels and subclinical atherosclerosis risk factors such as soluble CD40 ligand (sCD40L) and high-sensitivity CRP (hsCRP) in migraine patients during interictal period. Fifty female migraine patients and age-matched 25 female control cases were enrolled in the study. Migraine diagnosis was settled according to the ICHD-II diagnostic criteria. A questionnaire was completed about the existence of vascular risk factors. Serum samples were used to measure sCD40L, hsCRP and prolactin levels. No difference was found between the prolactin levels of the migraine patients and the controls. The sCD40L levels were significantly higher in migraine patients (p < 0.001). High-sensitivity CRP levels showed no difference between the groups. There was no correlation between prolactin, sCD40L, and hs-CRP levels in migraine patients. We consider that the migraine patients are prone to subclinical atherosclerosis, but this tendency is independent of prolactin levels

A new optical waveguide for telecommunication application

Conducting polymer is a physical mixture of non-conducting polymer with electrically conducting material. Polyaniline is a class of conducting polymers that exists in four different forms depending on protonation and doping of the base. Starting with aniline, polyaniline is synthesized and doped with two different metals namely iron and aluminum separately. Using these films optical waveguides are fabricated. Their characteristics viz. Refractive index, propagation loss, number of modes etc., are studied using prism coupling technique. The results are discussed

Effects of human placental amnion derived mesenchymal stem cells on proliferation and apoptosis mechanisms in chronic kidney disease in the rat

Background and Objectives: The feature of chronic kidney failure (CKF) is loss of kidney functions due to erosion of healthy tissue and fibrosis. Recent studies showed that Mesenchymal stem cells (MSCs) differentiated into tubular epithelial cells thus renal function and structures renewed.Furthermore, MSCs protect renal function in CKF. Therefore, we aimed to investigate whether human amnion-derived mesenchymal stem cells (hAMSCs) can repair fibrosis and determine the effects on proliferation and apoptosis mechanisms in chronic kidney failure. Methods and Results: In this study, rat model of CKF was constituted by applying Aristolochic acid (AA). hAMSCs were isolated from term placenta amnion membrane and transplanted into tail vein of rats. At the end of 30 days and 60 days of recovery period, we examined expressions of PCNA, p57 and Parp-1 by western blotting. Immunoreactivity of PCNA, Ki67, IL-6 and Collagen type I were detected by immunohistochemistry. Besides, apoptosis was detected by TUNEL. Serum creatinine and urea were measured. Expressions of PCNA and Ki67 increased in hAMSC groups compared with AA group. Furthermore, expressions of PARP-1 apoptosis marker and p57 cell cycle inhibitory protein increased in AA group significantly according to control, hAMSC groups and sham groups. IL-6 proinflammatory cytokine increased in AA group significantly according to control, hAMSCs groups and sham groups. Expressions of Collagen type I protein reduced in hAMSCs groups compared to AA group. After hAMSC treatment, serum creatinine and urea levels significantly decreased compared to AA group. After injection of hAMSC to rats, Masson's Trichrome and Sirius Red staining showed fibrosis reduction in kidney. Conclusions: According to our results hAMSCs can be ameliorate renal failure. © 2019 by the Korean Society for Stem Cell Research.TYL-2014-131This study was supported by Research Foundation of Akdeniz University, Turkey (project number: TYL-2014-131)

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novel ornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-1 with NAA (0.0, 0.5, 1.0 mgl-1) and BA (0.0, 0.5, 1.0, 2.0 mgl-1) with IAA (0.0, 0.5, 1.0, mgl-1) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-1 BA and 1.0 mgl-1 NAA (70%) followed by 1.0 mgl-1 BA and 0.5 mgl-1 NAA (50%), 1.0 mgl-1 BA and 1.0 mgl-1 NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-1 BA and 0.5 mgl-1 IAA (43%) followed by 0.5 mgl-1 BA and 1.0 mgl-1 IAA (23%). © 2009 Academic Journals

Regeneration of begonia plantlets by direct organogenesis

The economic importance of ornamentals worldwide suggests a bright future for ornamental breeding. Rapid progress in plant molecular biology has great potentials to contribute to the breeding of novelornamental plants utilizing recombinant DNA technology. The plant cell, tissue or organ culture of many ornamental species and their regeneration are essential for providing the material and systems for their genetic manipulation, and this is therefore the first requirement of genetic engineering. In this research, different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-¹ with NAA (0.0, 0.5, 1.0 mgl-¹) and BA (0.0, 0.5, 1.0, 2.0 mgl-¹) with IAA (0.0, 0.5, 1.0, mgl-¹) were investigated to optimize regeneration of Begonia elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing 2.0 mgl-¹ BA and 1.0 mgl-¹ NAA (70%) followed by 1.0 mgl-¹ BA and 0.5 mgl-¹ NAA (50%), 1.0 mgl-¹ BA and 1.0 mgl-¹ NAA (20%) in BA - NAA combination. The media with BA - IAA combination showed that the best regeneration was 0.5 mgl-¹ BA and 0.5 mgl-¹ IAA (43%) followed by 0.5 mgl-¹ BA and 1.0 mgl-¹ IAA (23%)