IMMUNOFLUORESCENT STUDY OF ACUTE RESPIRATORY DISEASES (ARD) SPREADED AMONG THE POPULATION OF VARNA AND DEVNYA DURING THE PERIOD SEPTEMBER 1980 - MARCH 1981

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JMMUNOFLUORESCENT DIAGNOSIS OF INFLUENZA INFECTIONS

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SORPTION OF ADENOVIRUS TYPE 7 AND ECHO-VIRUS TYPE 19 ONTO A NATURAL MINERAL SORBENT

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ETIOLOGICAL AND EPIDEMIOLOGICAL INVESTIGATIONS OF INFLUENZA IN VARNA AND VARNA DISTRICT DURING THE EPIDEMIC WAVE IN 1974—1975

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Variable stars in the globular cluster M13

Results of a search for variable stars in the central region of the globular cluster M13 are presented. Prior to this study, 36 variable and suspected variable stars were known in this cluster (Osborn 2000; Clement et al. 2001). Of these stars, five were not observed by us. We find v3, v4, v10, v12, and v13 to be constant in light. Surprisingly, only two out of the ten variable star candidates of Kadla et al. (1980) appear to be variable. Both are RRc variables. Additionally, three RR Lyrae stars and one SX Phoenicis variable are discovered. Three close frequencies are detected for an RRc star v36. It appears that this variable is another multi-periodic RR Lyrae star pulsating in non-radial modes. Light curves of the three known BL Herculis stars and all known RR Lyrae stars are presented. The total number of known RR Lyrae stars in M13 is now nine. Only one is an RRab star. The mean period of RRc variables amounts to 0.36 +/- 0.05 d, suggesting that M13 should be included in the group of Oosterhoff type II globular clusters. Mean V magnitudes and ranges of variation are derived for seven RR Lyrae and three BL Herculis variables. Almost all observed bright giants show some degree of variability. In particular, we confirm the variability of two red giants announced to be variable by Osborn (2000) and in addition find five new cases.Comment: 10 pages, 9 figures, 5 tables, accepted for publication in AA

Silver Flowerlike Structures for Surface-Enhanced Raman Spectroscopy

Micro-and nanoflowers are a class of materials composed of particles with high surface-to-volume ratio. They have been extensively studied in the last decade due to simple preparation protocols and promising applications in biosensing, as drug delivery agents, for water purification, and so on. Flowerlike objects, due to their highly irregular surface, may act also as plasmonic materials, providing resonant coupling between optical waves and surface plasmon excitations. This fact allows us to infer the possibility to use micro-and nanoflowers as effective surface-enhanced Raman scattering (SERS) substrate materials. Here, we report on the design and Raman enhancement properties of silver flowerlike structures, deposited on aluminum surface. A simple and cost-effective fabrication method is described, which leads to SERS substrates of high developed surface area. The morphology of the silver flowers on a nanoscale is characterized by self-organized quasiperiodic stacks of nanosheets, which act as plasmonic cavity resonators. The substrates were tested against rhodamine-6G (R6G) water solutions of concentration varying between 10−3 M and 10−7 M. Optimal SERS enhancement factors of up to 105 were established at R6G concentrations in the 10−6–10−7 M range. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.This research was funded by the Bulgarian-Russian bilateral project through the contracts KP-06-15/27.09.2019 Russia with the Bulgarian National Fund of Scientific Research (G.G.T, N.D.T., S.C.R., and V.G.I.) and RFBR project No. 19-52-18008 Bulg (A.V.L.)

Variable Stars in Galactic Globular Clusters

Based on a search of the literature up to May 2001, the number of known variable stars in Galactic globular clusters is approximately 3000. Of these, more than 2200 have known periods and the majority (approximately 1800) are of the RR Lyrae type. In addition to the RR Lyrae population, there are approximately 100 eclipsing binaries, 120 SX Phe variables, 60 Cepheids (including population II Cepheids, anomalous Cepheids and RV Tauri) and 120 SR/red variables. The mean period of the fundamental mode RR Lyrae variables is 0.585, for the overtone variables it is 0.342 (0.349 for the first-overtone pulsators and 0.296 for the second-overtone pulsators) and approximately 30% are overtone pulsators. These numbers indicate that about 65% of RR Lyrae variables in Galactic globular clusters belong to Oosterhoff type I systems. The mean period of the RR Lyrae variables in the Oosterhoff type I clusters seems to be correlated with metal abundance in the sense that the periods are longer in the more metal poor clusters. Such a correlation does not exist for the Oosterhoff type II clusters. Most of the Cepheids are in clusters with blue horizontal branches.Comment: 45 pages, 10 figures, to be published in AJ November 200

Species History Masks the Effects of Human-Induced Range Loss – Unexpected Genetic Diversity in the Endangered Giant Mayfly Palingenia longicauda

Freshwater biodiversity has declined dramatically in Europe in recent decades. Because of massive habitat pollution and morphological degradation of water bodies, many once widespread species persist in small fractions of their original range. These range contractions are generally believed to be accompanied by loss of intraspecific genetic diversity, due to the reduction of effective population sizes and the extinction of regional genetic lineages. We aimed to assess the loss of genetic diversity and its significance for future potential reintroduction of the long-tailed mayfly Palingenia longicauda (Olivier), which experienced approximately 98% range loss during the past century. Analysis of 936 bp of mitochondrial DNA of 245 extant specimens across the current range revealed a surprisingly large number of haplotypes (87), and a high level of haplotype diversity (). In contrast, historic specimens (6) from the lost range (Rhine catchment) were not differentiated from the extant Rába population (, ), despite considerable geographic distance separating the two rivers. These observations can be explained by an overlap of the current with the historic (Pleistocene) refugia of the species. Most likely, the massive recent range loss mainly affected the range which was occupied by rapid post-glacial dispersal. We conclude that massive range losses do not necessarily coincide with genetic impoverishment and that a species' history must be considered when estimating loss of genetic diversity. The assessment of spatial genetic structures and prior phylogeographic information seems essential to conserve once widespread species