Investigations on Anti-Diabetic Medicinal Plants Used by Tribal Inhabitants of Nalamankadai, Chitteri Reserve Forest, Dharmapuri, India

An ethnobotanical survey was conducted to collect information about medicinal plants used for the treatment of diabetics and associated complications by tribals in Nalamankadai village of Chitteri Hills, Dharmapuri District. The indigenous knowledge of local traditional healers and native plants used for the treatment of diabetics related health disorders were collected through questionnaire and personal interviews. A total of 10 informants with in the age group of 50 to 68 were interviewed, among them two were tribal practitioners. The investigation revealed that, the traditional healers and the inhabitants use 29 species of plants distributed in 28 genera belonging to 22 families to treat diabetics and related complications. Results depict that fresh plant materials were invariably preferred for the treatment of long term complications associated with diabetics. Anti-diabetic medicinal plants used by Malayali’s in Chitteri have been listed along with plant parts used

Extraction and partial purification of secondary metabolites from endophytic actinomycetes of marine green algae Caulerpa racemosa against multi drug resistant uropathogens

The aim of this study is to identify potential antibacterial compounds of endophytic actinomycetes (EA) from marine algae Caulerpa racemosa for inhibit the multi drug resistant (MDR) Gram negative bacteria (GNB) including Eschericia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Proteus mirabilis (P. mirabilis), Klebsiella pnumonia (K. pnumoniae) and Enterobacter sp., which were confirmed by UTI panel antibiotic discs. Among the 100 EA, five strains were showed better antogonistic activity against all the selected uropathogens. In the ethyl acetate extract, the NMS 5 strains of EA showed excellent anti-bacterial activity against all the MDR uropathogen. Further, the NMS 5 extract was partially purified from thin layar chramatogaraphy (TLC) and spot 3 of TLC crude compound showed 14, 12mm zone of inhibition against both the uropathogens. In addition, the minimum inhibition concentration (MIC) of crude exhibited the inhibition percentage was 87 and 84 at 120 μg/ mL concentration. The MIC was confirmed by minimum bactericidal concentration (MBC) and it was indicated same as MIC. The treated NMS 5 crude compound of P. aeruginosa and K. pneumonia cells were showed more dead cells, intracellular damage and morphological damage with disaggregated cells by confocal laser scanning electron microscope (CLSM) and scanning electron microscope (SEM). Hence, the present study was revealed that the algae derived EA have the potential anti-bacterial metabolites for inhibition of MDR bacteria and it can be applied for various other infections after further purification