Generation of stable Drosophila cell lines using multicistronic vectors

Insect cell culture is becoming increasingly important for applications including recombinant protein production and cell-based screening with chemical or RNAi libraries. While stable mammalian cell lines expressing a protein of interest can be efficiently prepared using IRES-based vectors or viral-based approaches, options for stable insect cell lines are more limited. Here, we describe pAc5-STABLEs, new vectors for use in Drosophila cell culture to facilitate stable transformation. We show that viral-derived 2A-like (or "CHYSEL") peptides function in Drosophila cells and can mediate the multicistronic expression of two or three proteins of interest under control of the Actin5C constitutive promoter. The current vectors allow mCherry and/or GFP fusions to be generated for positive selection by G418 resistance in cells and should serve as a flexible platform for future applications

Dosimetric and radiobiological evaluation of four radiation techniques in preoperative rectal cancer radiotherapy

Purpose: To compare tumour dose distribution, conformality, homogeneity, normal tissue avoidance, tumour control probability (TCP) and normal tissue complication probability (NTCP) using 3D conformal radiation therapy (3DCRT), 3- and 4-field intensity-modulated radiation therapy (IMRT) and volumetric-modulated arc therapy (VMAT) in patients with locally advanced rectal cancer. Materials and methods: Twenty-four patients staged T 1-3 N + M 0 with locally advanced rectal cancer underwent neoadjuvant chemoradiation therapy. Four different radiotherapy plans were prepared for each patient: 3DCRT, 3- and 4-field IMRT and VMAT are evaluated for target distribution using CI and homogeneity index (HI), normal tissue avoidance using D max, V 45, V 40, V 50 and TCP and NTCP using the Lyman-Kutcher-Burman model. Results: VMAT has better HI (HI = 1·32) and 3DCRT exhibited better conformality (CI = 1·05) than the other radiotherapy techniques. With regard to normal tissue avoidance, all radiotherapy plans met the constraints. D max in the 3DCRT plans was statistically significant (p = 0·04) for bladder and no significant differences in V 40 and V 50. In the bowel bag, no significant differences in D max for any radiotherapy plan and V 40 was lower in 3DCRT than VMAT (p = 0·024). In the case of femoral heads, 3DCRT has a statistically significant lower dose on D max than 4-field IMRT (p = 0·00 « 0·05). VMAT has the biggest TCP (80·76%) than the other three radiotherapy plans. With regard to normal tissue complications, probabilities were shown to be very low, of the order of 10-14 and 10-41 for bowel bag and femoral heads respectively. Conclusions: It can be concluded that 3DCRT plan improves conformity and decreases radiation sparing in the organ at risks, but the VMAT plan exhibits better homogeneity and greater TCP. © 2021 Cambridge University Press. All rights reserved

Assessment of energy-efficient appliances: A review of the technologies and policies in India's residential sector

The promotion of energy efficiency has been at the forefront of the energy policy agenda. New technological inventions and increasing environmental concerns related to contemporary energy policy are the main drivers of the adoption of more energy‐efficient appliances in domestic sector. Additionally, the mandatory star labeling program and incentive design mechanisms are also raising awareness and motivation for their use, thus contributing to the reduction of energy consumption and greenhouse gas emissions. Sustainable energy policies generally pursue programs aiming for energy efficiency among domestic appliances. In India there are currently nine domestic electrical appliances/end‐uses certified with star labeling programs, in particular lighting sources, refrigerators, air conditioners, water heaters, televisions, computers, washing machines, ceiling fans, and water pumps. This study reviews main issues affecting selection of energy‐efficient technologies in India's domestic sector highlighting the main challenges impacting design of energy efficiency policies and programs in the country.FCT/Portugal, Grant/Award Number: SFRH/BD/52308/201

Applying a multicriteria satisfaction analysis to an international airport

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