Howard University in the War

https://dh.howard.edu/hu_pub/1002/thumbnail.jp

Flock testing : weighing, sampling and measurement procedures for fleece measurement

An outline of shearing-shed procedure for the collection of greasy fleece weight records and sampling of the fleece for detailed measurement by the Department of Agriculture Flock Testing Service for Merino stud breeders

The role of fleece measurement in selecting merino sheep

MANY things determine how much wool a sheep can grow. These include the amount and quality of pasture available or how the sheep are fed, the stocking rate, the general health and thrift of the sheep and other management considerations such as cropping and the time of lambing

Genomic integration of transgenes using UV irradiation in Pristionchus pacificus

Transgenes are widely used throughout molecular biology for numerous applications. In Caenorhabditis elegans, stable transgenes are usually generated by microinjection into the germline establishing extrachromosomal arrays. Furthermore, numerous technologies exist to integrate transgenes into the C. elegans genome. In the nematode Pristionchus pacificus, transgenes are possible, however, their establishment is less efficient and dependent on the formation of complex arrays containing the transgene of interest and host carrier DNA. Additionally, genomic integration has only been reported via biolistic methods. Here we describe a simple technique using UV irradiation to facilitate the integration of transgenes into the P. pacificus genome

Effects of ultrasonic treatments on the polyphenol and antioxidant content of spinach extracts

The objective was to test ultrasound treatments on spinach leaves during extraction, and conventional extraction was used as a control. The effects of different combinations of the ultrasonic water bath factors tested on phenolic compound yields included frequency (37 and 80 kHz), exposure time (5, 10, 15, 20, 25 and 30 min), temperature (30, 40, and 50 C), and ultrasonic power (30%, 50%, and 70%). The best conditions for extraction yields were ultrasonic frequency of 37 kHz, extraction time of 30 min, reaction temperature of 40 C, and ultrasonic power of 50%. The mean yield (mg/100 g), total phenol (mg gallic acid/g DW), flavonoids (mg/g DW), % DPPH free-radical scavenging activity, and % ferric reducing antioxidant power were all high (64.88 ± 21.84, 33.96 ± 11.30, 27.37 ± 11.85, 64.18 ± 16.69 and 70.25 ± 9.68). Treatments were significantly different. The interaction among the ultrasonic parameters was significant. Temperature and power had significant effects on all other dependent variables

Gamification for servitization

The study described in this paper has set out to explore gamification from a servitization perspective to understand the opportunities and research challenges. A systematic literature review has been conducted to capture key characteristics of differing forms of gamification processes in a servitization context. The findings of our study focus on two area: (1) structural, organization’s physical bricksand-mortar attribute and (2) infrastructural, policies and practices in how structural aspects of servitization are to be managed. Six key findings are presented and collectively these contribute to our understanding of the broader gamification technologies that can help to transform servitization. The contribution of our research is twofold. First it captures a set of theoretical framework for analysing gamification in servitization context. Second, it provide an in-depth roadmap in how gamification can be applied to target major challenges in servitization

Gamification:using gaming mechanics to promote a business

The use of digital games and gamification has demonstrable potential to improve many aspects of how businesses provide training to staff, operate, and communicate with consumers. However, a need still exists for the benefits and potential of adopting games and gamification be effectively communicated to decision-makers across sectors. This article provides a structured review of existing literature on the use of games in the business sector, seeking to consolidate findings to address research questions regarding their perception, proven efficacy, and identify key areas for future work. The findings consolidate evidence showing serious games can have a positive and valuable impact in multiple areas of a business, including training, decision-support, and consumer outreach. They also highlight the challenges and pitfalls of applying serious games and gamification principles within a business context, and discuss the implications of development and evaluation methodologies on the success of a game-based solution

Simultaneous extraction, optimization, and analysis of flavonoids and polyphenols from peach and pumpkin extracts using a TLC-densitometric method

Background: The use of medicinal plants has been reported throughout human history. In the fight against illnesses, medicinal plants represent the primary health care system for 60 % of the world’s population. Flavonoids are polyphenolic compounds with active anti-microbial properties; they are produced in plants as pigments. Quercetin, myricetin, and rutin are among the most well-known and prevalent flavonoids in plants, with an antioxidant activity capable of decreasing the oxidation of low density lipoproteins [LDLs]. To date, this research is the first of its kind to employ a coupled thin-layer chromatography (TLC) and a densitometric quantification method with a Box-Behnken design (BBD) response surface methodology (RSM) for optimization of ultrasonic-assisted extraction and determination of rutin and quercetin from peach and ellagic acid and myricetin from pumpkin fruits. Results: The effect of process variables (extraction temperature (°C), extraction power (%) and extraction time (min)) on ultrasound-assisted extraction (UAE) were examined by using BBD and RSM. TLC followed by Quantity-One™ (BioRad) image analysis as a simple and rapid method was used for identification and quantification of the compounds in complex mixtures. The results were consistent under optimal conditions among the experimental values and their predicted values. A mass spectrometry (MALDI-TOF MS) technique was also used to confirm the identity of the natural products in the TLC spots resolved. Conclusion: The results show that the coupled TLC-densitometric methods & BBD can be a very powerful approach to qualitative and quantitative analysis of; rutin and quercetin from peach extracts; and ellagic acid and myricetin contents from pumpkin extracts

Protein Fingerprinting: A Domain-Free Approach to Protein Analysis

An alternative method for analyzing proteins is proposed. Currently, protein search engines available on the internet utilize domains (predefined sequences of amino acids) to align proteins. The method presented converts a protein sequence with the use of 1200 numeric codes that represent a unique three—amino-acid protein sequence. Each numeric code starts with one of three specific amino acids, followed by any two additional amino acids. With the use of the FPC (FingerPrinted Contig) program, the total protein database (including “redundant” records) from the National Center for Biotechnology Information (NCBI) has been processed and placed into “bins/contigs” based on associations of these triplet codes. When analyzed with FPC, proteins are “contigged” together based on the number of shared fragments, regardless of order. These associations were supported by additional analysis with the standard BLASTP utility from NCBI. Within the created contig sets, there are numerous examples of proteins (allotypes and orthotypes) that have evolved into different, seemingly unrelated proteins. The power of this domain-free technique has yet to be explored; however, the ability to bin proteins together with no a priori knowledge of domains may prove a powerful tool in the characterization of the hundreds of thousands of available, yet undescribed expressed protein and open reading frame sequences

Effect of drip irrigation circuits design and lateral line length on: II-flow velocity and velocity head

The objectives of the work were to study the effect of drip irrigation circuits (DIC) and lateral lines lengths (LLL) on: Flow velocity (FV) and velocity head (VH). Laboratory tests were con- ducted at Irrigation Devices and Equipments Tests Laboratory, Agricultural Engineering Research Institute, Agriculture Research Center, Giza, Egypt. The experimental design of laboratory experiments was split in randomized complete block design with three replicates. Laboratory tests carried out on three irrigation lateral lines 40, 60, 80 m (LLL1, LLL2; LLL3) under the following three drip irrigation circuits (DIC): a) one manifold for lateral lines or closed circuits with one manifold of drip irrigation system (CM1DIS); b) closed circuits with two manifolds for lateral lines (CM2DIS), and c) traditional drip irrigation system (TDIS) as a control. Concerning FV values, DIC and LLL treatments could state in the following ascending orders: TDIS \u3c CM1DIS \u3c CM2DIS and LLL1 \u3c LLL2 \u3c LLL3, respectively. FV varied from 0.593 m·sec–1 to 1.376 m·sec–1. i.e. FV \u3c 5 ft·sec–1 and this is necessary to avoid the effect of water hammer in the main and sub-main lines, but in lateral line, it can cause silt and clay precipitation problems. The differences in FV among DIC and LLL were significant at the 1% level. The effect of interaction: DIC X LLL on FV values, were significant at the 1% level. The maximum and minimum values of FV were noticed in these interactions: CM2DIS X LLL3 and TDIS X LLL1, respectively. The following ascending orders TDIS \u3c CM1DIS \u3c CM2DIS and LLL1 \u3c LLL2 \u3c LLL3 expressed their effects on VH respectively. Differences in VH among DIC and/or LLL were significant at the 1% with few exceptions. The effects of interactions: DIC X LLL on VH were significant at the 1% level in some cases. The maximum and minimum values of VH were found in the interactions: CM2DIS X LLL3 and TDIS X LLL1, respectively