Species- and organ-specificity of secretory proteins derived from human prostate and seminal vesicles

Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal antibody against β‐MSP were used for immunocytochemical detection of the respective antigens in different organs from different species. SG immunoreactivity was detected in the epithelium of the pubertal and adult human and in monkey seminal vesicle, ampulla of the vas deferens, and ejaculatory duct. PAP, β‐MSP, and PSA immunoreactivities were detected in the pubertal and adult human prostate and the cranial and caudal monkey prostate. With the exception of a weak PSA immunoreactivity in the proximal portions of the ejaculatory duct, none of the latter antisera reacted with seminal vesicle, ampullary, and ejaculatory duct epithelium. Among the non‐primate species studied (dog, bull, rat, guinea pig) only the canine prostatic epithelium displayed a definite immunoreactivity with the PAP antibody and a moderate reaction with the PSA antibody. No immunoreaction was seen in bull and rat seminal vesicle and canine ampulla of the vas deferens with the SG antibody. The same was true for the (ventral) prostate of rat, bull, and dog for β‐MSP. The epithelium of the rat dorsal prostate showed a slight cross‐reactivity with the monoclonal antibody against β‐MSP and one polyclonal antibody against PSA. The findings indicate a rather strict species‐dependent expression of human seminal proteins which show some similarities in primates, but only marginal relationship to species with different physiology of seminal fluid

The high temperature erosion-corrosion behaviour of industrial thermal sprayed coatings

Describes the high temperature erosion-corrosion behaviour of industrial thermal sprayed coatings

Meerjarige wortelonkruiden : onderzoeksplan

Er zijn op akkerbouwbedrijven, vooral op biologisch, problemen met de bestrijding van meerjarige wortelonkruiden. Het probleem wordt ook steeds groter. Door middel van biologische grond ontsmetting (BGO) wordt het aantal meerjarige wortelonkruiden verminderd, maar hoeveel en hoe sterk dat is, is niet bekend. Het effect van BGO werd voornamelijk onderzocht op het onkruid akkermelkdistel. Dit onderzoek werd uitgevoerd door PPO-AGV te Lelystad geholpen door leerlingen van de CAH Dronten. Het onderzoek is een echte proef van PPO en geeft studenten de kans om te leren hoe het in de praktijk gaat met uitvoering en verwerking van gegevens in het onderzoek

The erosion of functionally graded coatings under fluidized bed conditions

Details the erosion of functionally graded coatings under fluidized bed conditions

CRINOIDS FROM THE NADA MEMBER OF THE BORDEN FORMATION (LOWER MISSISSIPPIAN) IN EASTERN KENTUCKY

ABSTRACT—Thirty-four crinoid species, including four new species, are reported from the Nada Member of the Borden Formation in eastern Kentucky. The dominant crinoid group is monobathrids (18 species), but diplobathrids, disparids, cladids, and flexibles are also present. The four new species are the camerates Blairocrinus protuberatus, Uperocrinus acuminatus, and Aorocrinus nodulus, and the cladid Atelestocrinus kentuckyensis. The majority of the species in this fauna were previously known from what has traditionally been considered the ‘‘upper part’ ’ of the Burlington Limestone in the Mississippi River Valley and what is now recognized as the Burlington Pelmatozoan Assemblage III. The current study confirms the conclusion of Lane and DuBar (1983) that the Nada is middle Osagean in age, being deposited earlier than the well-documented Borden delta crinoid assemblages of north-central Kentucky and Indiana. The middle Osagean age of the fauna indicates a previously unrecognized unconformity between the Nada and the overlying Meramecian-age Renfro Member of the Slade Formation. In addition, this is the first well-documented middle Osagean fauna from a siliciclastic facies in North America. The dominance by camerate crinoids is enigmatic, but may be related to either a low rate of sedimentation or greater larval dispersal abilities

Optimized Layout and Roadway support planning with integrated intelligent software

With declining ore grades, exhaustion of near surface reserves and rising labour costs the underground coal and metal mining industry in the European Union requires efficient planning tools that will improve its performance since mechanisation has almost approached 100% so that machine development will only give marginal improvements in performance. Major improvements can only be achieved by the re-development of the whole production process through careful and precise mine planning that will utilise simulation and production system's analysis