500 research outputs found
Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation
A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on Percoll gradient step. The intactness and purity of the chloroplasts isolated from leaves of low irradiance-acclimated plants by using this protocol (confirmed by phase contrast microscopy as well as enzymatic and immunological approaches) allows plausible studies on low irradiance-dependent Rubisco regulation
Twisted equivariant K-theory, groupoids and proper actions
In this paper we define twisted equivariant K-theory for actions of Lie
groupoids. For a Bredon-compatible Lie groupoid, this defines a periodic
cohomology theory on the category of finite CW-complexes with equivariant
stable projective bundles. A classification of these bundles is shown. We also
obtain a completion theorem and apply these results to proper actions of
groups.Comment: 26 page
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In vivo fiber tractography of the right and left ventricles using diffusion tensor MRI of the entire human heart
From qualitative to quantitative tractography: a novel method to measure variation and error in diffusion mr tractography datasets of the myocardium
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Myocardial infarct delineation in vivo using diffusion tensor MRI and the tractographic propagation angle
Assessing Music Perception in Young Children: Evidence for and Psychometric Features of the M-Factor
Given the relationship between language acquisition and music processing, musical perception (MP) skills have been proposed as a tool for early diagnosis of speech and language difficulties; therefore, a psychometric instrument is needed to assess music perception in children under 10 years of age, a crucial period in neurodevelopment. We created a set of 80 musical stimuli encompassing seven domains of music perception to inform perception of tonal, atonal, and modal stimuli, in a random sample of 1006 children, 6–13 years of age, equally distributed from first to fifth grades, from 14 schools (38% private schools) in So Paulo State. The underlying model was tested using confirmatory factor analysis. A model encompassing seven orthogonal specific domains (contour, loudness, scale, timbre, duration, pitch, and meter) and one general music perception factor, the “m-factor,” showed excellent fit indices. The m-factor, previously hypothesized in the literature but never formally tested, explains 93% of the reliable variance in measurement, while only 3.9% of the reliable variance could be attributed to the multidimensionality caused by the specific domains. The 80 items showed no differential item functioning based on sex, age, or enrolment in public vs. private school, demonstrating the important psychometric feature of invariance. Like Charles Spearman's g-factor of intelligence, the m-factor is robust and reliable. It provides a convenient measure of auditory stimulus apprehension that does not rely on verbal information, offering a new opportunity to probe biological and psychological relationships with music perception phenomena and the etiologies of speech and language disorders
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Correlation of DTI tractography with electroanatomic mapping in normal and infarcted myocardium
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Accelerated free-breathing diffusion tensor MRI of the entire human heart using spatiotemporal registration and retrospective image selection
Left ventricular remodeling following myocardial infarction revealed with a quantitative diffusion MRI tractography framework
A cardiac-tailored framework for 3D Diffusion Tensor MRI tractography is developed and used to characterize myofiber architecture in normal and remodeled myocardium. We show that myofibers in the subepicardium of the remote infarct zone become less oblique (more circumferential) as the heart dilates and remodels. This fiber realignment may play an important role in the loss of contractile function in the remote zone over time
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