1,140 research outputs found
Apoptosis in mouse fetal and neonatal oocytes during meiotic prophase one
Background
The vast majority of oocytes formed in the fetal ovary do not survive beyond birth.
Possible reasons for their loss include the elimination of non-viable genetic
constitutions arising through meiosis, however, the precise relationship between
meiotic stages and prenatal apoptosis of oocytes remains elusive. We studied oocytes
in mouse fetal and neonatal ovaries, 14.5–21 days post coitum, to examine the
relationship between oocyte development and programmed cell death during meiotic
prophase I.
Results
Microspreads of fetal and neonatal ovarian cells underwent immunocytochemistry for
meiosis- and apoptosis-related markers. COR-1 (meiosis-specific) highlighted axial
elements of the synaptonemal complex and allowed definitive identification of the
stages of meiotic prophase I. Labelling for cleaved poly-(ADP-ribose) polymerase
(PARP-1), an inactivated DNA repair protein, indicated apoptosis. The same oocytes
were then labelled for DNA double strand breaks (DSBs) using TUNEL. 1960
oocytes produced analysable results. .
Oocytes at all stages of meiotic prophase I stained for cleaved PARP-1 and/or TUNEL, or neither. Oocytes with fragmented (19.8%) or compressed (21.2%) axial
elements showed slight but significant differences in staining for cleaved PARP-1 and
TUNEL to those with intact elements. However, fragmentation of axial elements
alone was not a good indicator of cell demise. Cleaved PARP-1 and TUNEL staining
were not necessarily coincident, showing that TUNEL is not a reliable marker of apoptosis in oocytes.
Conclusions
Our data indicate that apoptosis can occur throughout meiotic prophase I in mouse
fetal and early postnatal oocytes, with greatest incidence at the diplotene stage.
Careful selection of appropriate markers for oocyte apoptosis is essential
A homological interpretation of the transverse quiver Grassmannians
In recent articles, the investigation of atomic bases in cluster algebras
associated to affine quivers led the second-named author to introduce a variety
called transverse quiver Grassmannian and the first-named and third-named
authors to consider the smooth loci of quiver Grassmannians. In this paper, we
prove that, for any affine quiver Q, the transverse quiver Grassmannian of an
indecomposable representation M is the set of points N in the quiver
Grassmannian of M such that Ext^1(N,M/N)=0. As a corollary we prove that the
transverse quiver Grassmannian coincides with the smooth locus of the
irreducible components of minimal dimension in the quiver Grassmannian.Comment: final version, 7 pages, corollary 1.2 has been modifie
Chronicles of Oklahoma
Article describes life in territorial Skullyville, Oklahoma, a town that was the original location of the first agency for the Choctaws in Indian Territory. G. E. Hartshorne, M.D. uses personal recollections of the town to describe its layout and people
Log canonical thresholds of Del Pezzo Surfaces in characteristic p
The global log canonical threshold of each non-singular complex del Pezzo
surface was computed by Cheltsov. The proof used Koll\'ar-Shokurov's
connectedness principle and other results relying on vanishing theorems of
Kodaira type, not known to be true in finite characteristic.
We compute the global log canonical threshold of non-singular del Pezzo
surfaces over an algebraically closed field. We give algebraic proofs of
results previously known only in characteristic . Instead of using of the
connectedness principle we introduce a new technique based on a classification
of curves of low degree. As an application we conclude that non-singular del
Pezzo surfaces in finite characteristic of degree lower or equal than are
K-semistable.Comment: 21 pages. Thorough rewrite following referee's suggestions. To be
published in Manuscripta Mathematic
A simple remark on a flat projective morphism with a Calabi-Yau fiber
If a K3 surface is a fiber of a flat projective morphisms over a connected
noetherian scheme over the complex number field, then any smooth connected
fiber is also a K3 surface. Observing this, Professor Nam-Hoon Lee asked if the
same is true for higher dimensional Calabi-Yau fibers. We shall give an
explicit negative answer to his question as well as a proof of his initial
observation.Comment: 8 pages, main theorem is generalized, one more remark is added,
mis-calculation and typos are corrected etc
The hidden costs of dietary restriction: Implications for its evolutionary and mechanistic origins
Dietary restriction (DR) extends life span across taxa. Despite considerable research, universal mechanisms of DR have not been identified, limiting its translational potential. Guided by the conviction that DR evolved as an adaptive, pro-longevity physiological response to food scarcity, biomedical science has interpreted DR as an activator of pro-longevity molecular pathways. Current evolutionary theory predicts that organisms invest in their soma during DR, and thus when resource availability improves, should outcompete rich-fed controls in survival and/or reproduction. Testing this prediction in Drosophila melanogaster (N > 66,000 across 11 genotypes), our experiments revealed substantial, unexpected mortality costs when flies returned to a rich diet following DR. The physiological effects of DR should therefore not be interpreted as intrinsically pro-longevity, acting via somatic maintenance. We suggest DR could alternatively be considered an escape from costs incurred under nutrient-rich conditions, in addition to costs associated with DR
On the classification of Kahler-Ricci solitons on Gorenstein del Pezzo surfaces
We give a classification of all pairs (X,v) of Gorenstein del Pezzo surfaces
X and vector fields v which are K-stable in the sense of Berman-Nystrom and
therefore are expected to admit a Kahler-Ricci solition. Moreover, we provide
some new examples of Fano threefolds admitting a Kahler-Ricci soliton.Comment: 21 pages, ancillary files containing calculations in SageMath; minor
correction
A Product Formula for the Normalized Volume of Free Sums of Lattice Polytopes
The free sum is a basic geometric operation among convex polytopes. This note
focuses on the relationship between the normalized volume of the free sum and
that of the summands. In particular, we show that the normalized volume of the
free sum of full dimensional polytopes is precisely the product of the
normalized volumes of the summands.Comment: Published in the proceedings of 2017 Southern Regional Algebra
Conferenc
Affine T-varieties of complexity one and locally nilpotent derivations
Let X=spec A be a normal affine variety over an algebraically closed field k
of characteristic 0 endowed with an effective action of a torus T of dimension
n. Let also D be a homogeneous locally nilpotent derivation on the normal
affine Z^n-graded domain A, so that D generates a k_+-action on X that is
normalized by the T-action. We provide a complete classification of pairs (X,D)
in two cases: for toric varieties (n=\dim X) and in the case where n=\dim X-1.
This generalizes previously known results for surfaces due to Flenner and
Zaidenberg. As an application we compute the homogeneous Makar-Limanov
invariant of such varieties. In particular we exhibit a family of non-rational
varieties with trivial Makar-Limanov invariant.Comment: 31 pages. Minor changes in the structure. Fixed some typo
Small bound for birational automorphism groups of algebraic varieties (with an Appendix by Yujiro Kawamata)
We give an effective upper bound of |Bir(X)| for the birational automorphism
group of an irregular n-fold (with n = 3) of general type in terms of the
volume V = V(X) under an ''albanese smoothness and simplicity'' condition. To
be precise, |Bir(X)| < d_3 V^{10}. An optimum linear bound |Bir(X)|-1 <
(1/3)(42)^3 V is obtained for those 3-folds with non-maximal albanese
dimension. For all n > 2, a bound |Bir(X)| < d_n V^{10} is obtained when alb_X
is generically finite, alb(X) is smooth and Alb(X) is simple.Comment: Mathematische Annalen, to appea
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