THE ANTI-INFLAMMATORY EFFECT OF GARDI PATCH IN CARRAGEENAN-INDUCED PAW EDEMA OF RATS

Gardi -5 has been used in traditional Mongolian medicine as an analgesic and antibacterial medicine. The drug has been studied in chemical-pharmacological research. We have a new natural patch from the prepared Gardi-5. The present work was undertaken to evaluate the traditional drug Gardi patch for its anti-inflammatory activity.The method of Miho Sekiquichi was used to study acute inflammation. Rats in groups of five each were treated with vehicle, Gardi patch and Ketoprofen patch one hour prior to Carrageenan injection. 0.1 ml of 1% Carrageenan was injected into the sub plantar tissue of left hind paw of each rat. Swelling of carrageenan injected foot was measured at 0, 30, 60, 120, 180, 240, 300 mins using Plethysmometer (UGO Basile, Italy). The right hind paw was injected with 0.1 ml of vehicle.The Gardi patch significantly (p=0.000) inhibited carrageenan induced rat paw edema as compared to control group. In assay data, the TNF-α, PGE2 secretion in serum were highly elevated by carrageenan induction but administration of Gardi patch significantly reduced serum secretion of inflammatory mediators as compared to vehicle group. Gardi patch has an anti-inflammatory properties

SOME RESULT OF QUALITY PARAMETERS OF “GARDI” TRANSDERMAL PATCH

The aim of this study was to conduct standardization study and determine quality parameters of “Gardi” transdermal patch. We used “Gardi” transdermal patch consists of extract of radix Aconitum Kusnezoffii, extract of Terminalia chebula, extract of Saussurea lappa L., extract of Acorus calamus L. and Musk of Musk deer. We used some biologically active compounds of the transdermal patch were revealed by TLC. And main biologically active compounds content of the transdermal patch were determined by spectrophotometric methods. The moisture, average weight and parameters of microbiology were determined by Mongolian National First Pharmacopoeia methods. The transdermal patch was light brown colour, unusual smell and rectangular shape. We determined aconitine, costunolide and gallic acid in “Gardi” transdermal patch by TLC. The retention time of aconitine was 63.04±0.2 minutes and amount was 0.169±0.009% by HPLC.The polyphenolic compounds by spectrophotometer method using Folin-Chiocalto reagent as 0.567±0.043%. Quality and safety parameters of “Gardi” transdermal patch determined as: moisture 27.28±0.65 %, average weight 3.969±0.196 g, thickness 0.83±0.2 mm, total bacteria 1х103, mould, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Staphylococcus aureus and Enterobacter were not detected in “Gardi” transdermal patch

Granites of the southern Mongolia Carboniferous arc : new geochronological and geochemical constraints

The crust in southern Mongolia is part of the Central Asian Orogenic Belt, a vast accretionary orogen that records the opening and closure of the Palaeo-asian Ocean in the late Proterozoic to Palaeozoic. The crustal evolution of the region is revealed in basement inliers that also contain intrusion-related porphyry ore bodies that are important mineral exploration targets. The Saykhandulaan inlier in Southeast Mongolia is a Devonian–Carboniferous segment of island-arc crust, which is dominantly composed of extrusive and sedimentary lithologies, but which also contains the Oyut Ulaan I-type quartz-monzonite intrusion. A U–Pb zircon age for the Oyut Ulaan monzonite indicates emplacement at 330.0 ± 0.5 Ma. To the east of the Saykhandulaan inlier, intrusive complexes dominate the neighbouring Mandakh inlier. New ages are presented for four of these plutons; the Bronze Fox granodiorite (333.6 ± 0.6 Ma); the Narin Hudag monzonite (333.2 ± 0.6 Ma); the Shuteen quartz monzonite (325.5 ± 1.0 Ma); and the North Mandakh granite (292.3 ± 0.5). The intrusive bodies of the Saykhandulaan and Mandakh inliers have two distinct geochronological and geochemical associations: 1) mid-Carboniferous I-type monzonites that constitute the most easterly intrusive expression of the Southern Mongolia Carboniferous Arc and, 2) Early Permian A-type and peralkaline granites that represent a post-orogenic phase of voluminous granite emplacement. Both groups are significantly younger than the nearby Oyu Tolgoi and Tsagaan Suvarga Cu-porphyry ore bodies, which have previously been dated as early- and late-Devonian respectively. The new data presented here provide constraints on the timing of the transition from island-arc magmatism to post-collisional extension-related magmatism in the region and possible controls on fertile and infertile granitoid intrusions with respect to Cu–Au mineralisation