Atmospheric refraction effects on the Norris and Keck multiobject spectrographs

Large field spectrographs are severely influenced by atmospheric refraction. We discuss the effect of differential refraction across the field as well as the chromatic effects for the Norris spectrograph under construction for use at Palomar Observatory and for a similar spectrograph for the Keck telescope on Mauna Kea

Atmospheric refraction effects on the Norris and Keck multiobject spectrographs

Large field spectrographs are severely influenced by atmospheric refraction. We discuss the effect of differential refraction across the field as well as the chromatic effects for the Norris spectrograph under construction for use at Palomar Observatory and for a similar spectrograph for the Keck telescope on Mauna Kea

Elucidation of the substrate binding site of Siah ubiquitin ligase

The Siah family of RING proteins function as ubiquitin ligase components, contributing to the degradation of multiple targets involved in cell growth, differentiation, angiogenesis, oncogenesis, and inflammation. Previously, a binding motif (degron) was recognized in many of the Siah degradation targets, suggesting that Siah itself may facilitate substrate recognition. We report the crystal structure of the Siah in complex with a peptide containing the degron motif. Binding is within a groove formed in part by the zinc fingers and the first two ß strands of the TRAF-C domain of Siah. We show that residues in the degron, previously described to facilitate binding to Siah, interact with the protein. Mutagenesis of Siah at sites of interaction also abrogates both in vitro peptide binding and destabilization of a known Siah target

Effect of pediatric influenza vaccination on antibiotic resistance in England and Wales

Vaccines against viral infections have been proposed to reduce prescribing of antibiotics and thereby help control resistant bacterial infections. However, by combining published data sources, we predict that pediatric live attenuated influenza vaccination in England and Wales will not substantially reduce antibiotic consumption or adverse health outcomes associated with antibiotic resistance

The Keck Low-Resolution Imaging Spectrometer

The Low Resolution Imaging Spectrometer (LRIS) for the Cassegrain focus of the Keck 10-m telescope on Mauna Kea is described. It has an imaging mode so it can also be used for taking direct images. The field of view in both spectrographic and imaging modes is 6 by 7.8 arcmin. It can be used with both conventional slits and custom-punched slit masks. The optical quality of the spectrograph is good enough to take full advantage of the excellent imaging properties of the telescope itself. The detector is a cooled back-illuminated Tektronics Inc. 2048 X 2048 CCD which gives a sampling rate of 4.685 pixels per arcsec. In the spectrographic mode the spectrograph has a maximum efficiency at the peak of the grating blaze of 32%-34% for the two lowest resolution gratings and 28% for the 1200 g mm^(-1) grating. This efficiency includes the detector but not the telescope or the atmosphere

The Software for the LRIS on the Keck 10-Meter Telescope

We discuss the software for the Low Resolution and Imaging Spectrograph, one of the first light instruments built for the Keck 10-meter telescope. Details of the CCD detector readout scheme, the motor control system, the user interface, the astrometric preparatory software, etc., are given

The Software for the LRIS on the Keck 10-Meter Telescope

We discuss the software for the Low Resolution and Imaging Spectrograph, one of the first light instruments built for the Keck 10-meter telescope. Details of the CCD detector readout scheme, the motor control system, the user interface, the astrometric preparatory software, etc., are given

Structure of the Janus Protein Human CLIC2

Chloride intracellular channel (CLIC) proteins possess the remarkable property of being able to convert from a water-soluble state to a membrane channel state. We determined the three-dimensional structure of human CLIC2 in its water-soluble form by X-ray crystallography at 1.8-Å resolution from two crystal forms. In contrast to the previously characterized CLIC1 protein, which forms a possibly functionally important disulfide-induced dimer under oxidizing conditions, we show that CLIC2 possesses an intramolecular disulfide and that the protein remains monomeric irrespective of redox conditions. Site-directed mutagenesis studies show that removal of the intramolecular disulfide or introduction of cysteine residues in CLIC2, equivalent to those that form the intramolecular disulfide in CLIC1, does not cause dimer formation under oxidizing conditions.We also show that CLIC2 forms pH-dependent chloride channels in vitro with higher channel activity at low pH levels and that the channels are subject to redox regulation. In both crystal forms, we observed an extended loop region from the C-terminal domain, called the foot loop, inserting itself into an interdomain crevice of a neighboring molecule. The equivalent region in the structurally related glutathione transferase superfamily corresponds to the active site. This so-called foot-in-mouth interaction suggests that CLIC2 might recognize other proteins such as the ryanodine receptor through a similar interaction

A Close Companion Search Around L Dwarfs Using Aperture Masking Interferometry and Palomar Laser Guide Star Adaptive Optics

We present a close companion search around 16 known early L dwarfs using aperture masking interferometry with Palomar laser guide star adaptive optics (LGS AO). The use of aperture masking allows the detection of close binaries, corresponding to projected physical separations of 0.6-10.0 AU for the targets of our survey. This survey achieved median contrast limits of ΔK ~ 2.3 for separations between 1.2λ/D-4λ/D and ΔK ~ 1.4 at 2/3λ/D. We present four candidate binaries detected with moderate-to-high confidence (90%-98%). Two have projected physical separations less than 1.5 AU. This may indicate that tight-separation binaries contribute more significantly to the binary fraction than currently assumed, consistent with spectroscopic and photometric overluminosity studies. Ten targets of this survey have previously been observed with the Hubble Space Telescope as part of companion searches. We use the increased resolution of aperture masking to search for close or dim companions that would be obscured by full aperture imaging, finding two candidate binaries. This survey is the first application of aperture masking with LGS AO at Palomar. Several new techniques for the analysis of aperture masking data in the low signal-to-noise regime are explored

Power tests of a string of magnets comprising a full cell of the Superconducting Super Collider

In this paper we describe the operation and testing of a string of magnets comprising a full cell of the Superconducting Super Collider (SSC). The full cell configuration composed of ten dipoles, two quadrupoles, and three spool pieces is the longest SSC magnet string ever tested. Although the tests of the full cell were undertaken after the SSC project was marked for termination, their completion was deemed necessary and useful to future efforts at other accelerator laboratories utilizing Superconducting magnets. The focus of this work is on the electrical and cryogenic performance of the string components and the quench protection system with an emphasis on solving some of the questions concerning electrical performance raised during the previous two experimental runs involving a half cell configuration