4,732 research outputs found
Studies on the mechanism of retinoid-induced pattern duplications in the early chick limb bud: temporal and spatial aspects.
All-trans-retinoic acid causes striking digit pattern changes when it is continuously released from a bead implanted in the anterior margin of an early chick wing bud. In addition to the normal set of digits (234), extra digits form in a mirror-symmetrical arrangement, creating digit patterns such as a 432234. These retinoic acid-induced pattern duplications closely mimic those found after grafts of polarizing region cells to the same positions with regard to dose-response, timing, and positional effects. To elucidate the mechanism by which retinoic acid induces these pattern duplications, we have studied the temporal and spatial distribution of all-trans-retinoic acid and its potent analogue TTNPB in these limb buds. We find that the induction process is biphasic: there is an 8-h lag phase followed by a 6-h duplication phase, during which additional digits are irreversibly specified in the sequence digit 2, digit 3, digit 4. On average, formation of each digit seems to require between 1 and 2 h. The tissue concentrations, metabolic pattern, and spatial distribution of all-trans-retinoic acid and TTNPB in the limb rapidly reach a steady state, in which the continuous release of the retinoid is balanced by loss from metabolism and blood circulation. Pulse-chase experiments reveal that the half-time of clearance from the bud is 20 min for all-trans-retinoic acid and 80 min for TTNPB. Manipulations that change the experimentally induced steep concentration gradient of TTNPB suggest that a graded distribution of retinoid concentrations across the limb is required during the duplication phase to induce changes in the digit pattern. The extensive similarities between results obtained with retinoids and with polarizing region grafts raise the possibility that retinoic acid serves as a natural "morphogen" in the limb
Shocks in asymmetric simple exclusion processes of interacting particles
In this paper, we study shocks and related transitions in asymmetric simple
exclusion processes of particles with nearest neighbor interactions. We
consider two kinds of inter-particle interactions. In one case, the
particle-hole symmetry is broken due to the interaction. In the other case,
particles have an effective repulsion due to which the particle-current-density
drops down near the half filling. These interacting particles move on a one
dimensional lattice which is open at both the ends with injection of particles
at one end and withdrawal of particles at the other. In addition to this, there
are possibilities of attachments or detachments of particles to or from the
lattice with certain rates. The hydrodynamic equation that involves the exact
particle current-density of the particle conserving system and additional terms
taking care of the attachment-detachment kinetics is studied using the
techniques of boundary layer analysis.Comment: 10 pages, 8 figure
Competition and norms: a self-defeating combination?
This paper investigates the effects of information feedback mechanisms on electricity and heating usage at a student hall of residence in London. In a randomised control trial, we formulate different treatments such as feedback information and norms, as well as prize competition among subjects. We show that information and norms lead to a sharp – more than 20% - reduction in overall energy consumption. Because participants do not pay for their energy consumption this response cannot be driven by cost saving incentives. Interestingly, when combining feedback and norms with a prize competition for achieving low energy consumption, the reduction effect – while present initially – disappears in the long run. This could suggest that external rewards reduce and even destroy intrinsic motivation to change behaviour
Particle Dispersion on Rapidly Folding Random Hetero-Polymers
We investigate the dynamics of a particle moving randomly along a disordered
hetero-polymer subjected to rapid conformational changes which induce
superdiffusive motion in chemical coordinates. We study the antagonistic
interplay between the enhanced diffusion and the quenched disorder. The
dispersion speed exhibits universal behavior independent of the folding
statistics. On the other hand it is strongly affected by the structure of the
disordered potential. The results may serve as a reference point for a number
of translocation phenomena observed in biological cells, such as protein
dynamics on DNA strands.Comment: 4 pages, 4 figure
Are stress-free membranes really 'tensionless'?
In recent years it has been argued that the tension parameter driving the
fluctuations of fluid membranes, differs from the imposed lateral stress, the
'frame tension'. In particular, stress-free membranes were predicted to have a
residual fluctuation tension. In the present paper, this argument is
reconsidered and shown to be inherently inconsistent -- in the sense that a
linearized theory, the Monge model, is used to predict a nonlinear effect.
Furthermore, numerical simulations of one-dimensional stiff membranes are
presented which clearly demonstrate, first, that the internal 'intrinsic'
stress in membranes indeed differs from the frame tension as conjectured, but
second, that the fluctuations are nevertheless driven by the frame tension.
With this assumption, the predictions of the Monge model agree excellently with
the simulation data for stiffness and tension values spanning several orders of
magnitude
Fluctuation spectrum of quasispherical membranes with force-dipole activity
The fluctuation spectrum of a quasi-spherical vesicle with active membrane
proteins is calculated. The activity of the proteins is modeled as the proteins
pushing on their surroundings giving rise to non-local force distributions.
Both the contributions from the thermal fluctuations of the active protein
densities and the temporal noise in the individual active force distributions
of the proteins are taken into account. The noise in the individual force
distributions is found to become significant at short wavelengths.Comment: 9 pages, 2 figures, minor changes and addition
The Green's function for the radial Schramm-Loewner evolution
We prove the existence of the Green's function for radial SLE(k) for k<8.
Unlike the chordal case where an explicit formula for the Green's function is
known for all values of k<8, we give an explicit formula only for k=4. For
other values of k, we give a formula in terms of an expectation with respect to
SLE conditioned to go through a point.Comment: v1: 16 pages, 0 figure
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