Pengembangan Keterampilan Sosial dan Kewirausahaan pada Organisasi Pemuda Keagamaan di Depok

Penelitian ini mengkaji kelompok pemuda dalam organisasi Muhammadiyah sebagai Faith Based Organization (FBO) menjadi agen Perubah dalam pengembangan masyarakat lokal di Kelurahan Cinangka, Depok, Jawa Barat. Penelitian ini membahas tentang proses intervensi kelompok pemuda dengan mempergunakan strategi penelitian aksi (participatory action research). Tahapan penelitian aksi terdiri dari tiga tahap, yaitu mengidentifikasi kebutuhan dan potensi (look), merancang program intervensi (think), dan mengimplementasikan program intervensi (act). Berdasarkan identifikasi masalah dan kebutuhan pada tahap look, penelitian ini menemukan potensi masyarakat dalam upaya menyelesaian permasalahan lingkungan. Selain pemuda yang tergabung dalam FBO, ada juga kelompok ibu yang terlibat. Tahap look memperlihatkan bahwa potensi pemuda dari FBO dapat maksimal apabila mendapatkan dukungan dari elemen lain, terutama orang tua di dalam komunitas sasaran. Kemudian melalui proses perencanaan kegiatan dalam tahap think bersama komunitas sasaran, terdapat beberapa kegiatan yang diimplementasikan dalam penelitian ini, yaitu pengembangan keterampilan sosial dan kewirausahaan. Penelitian ini menunjukkan bahwa intervensi kelompok pemuda dalam pengembangan komunitas melalui pengembangan keterampilan kewirausahaan, tidak dapat menanggalkan urgensi keterampilan sosial guna memperkuat peran pemuda dari FBO di komunitas.Kata Kunci: pengembangan masyarakat, intervensi kelompok pemuda, pengelolaan lingkungan, Faith Based Organization, keterampilan mikro.This study examines the youth groups in the Muhammadiyah organization as an Faith Based Organization (FBO) as agents of change in community development at Cinangka Village, Depok, West Java. By using a Participatory Action Research, this study discusses the process of youth group intervention in three stages, which covers needs and potencies assessment (look), action plan (think) and implementation (act). Based on ‘look' phase, this study found people's potential at their community which are youth in FBO and groups women. In this phase has identified that youth's potencies in FBO could be maximized if supported by parents. In the next stage through planning process, there were some activities that are implemented in this study which are development of social skills and entrepreneurship. This study has found that youth group intervention should also recognize social skills on strengthen the role of youth in FBO

Proton Pump Inhibitors Exert Anti-Allergic Effects by Reducing TCTP Secretion

BACKGROUND:Extracellular translationally controlled tumor protein (TCTP) is known to play a role in human allergic responses. TCTP has been identified outside of macrophages, in activated mononuclear cells, and in biological fluids from allergic patients. Even TCTP devoid of signal sequences, is secreted to extracellular environment by an yet undefined mechanism. This study is aimed at understanding the mechanism of TCTP release and its regulation. A secondary goal is to see if inhibitors of TCTP release can serve as potential anti-allergic asthmatic drugs. METHODOLOGY/PRINCIPAL FINDINGS:Using Western blotting assay in HEK293 and U937 cells, we found that TCTP secretion is reduced by omeprazole and pantoprazole, both of which are proton pump inhibitors. We then transfected HEK293 cells with proton pump expression vectors to search for the effects of exogeneously overexpressed H(+)/K(+)-ATPase on the TCTP secretion. Based on these in vitro data we checked the in vivo effects of pantoprazole in a murine model of ovalbumin-induced allergy. Omeprazole and pantoprazole reduced TCTP secretion from HEK293 and U937 cells in a concentration-dependent fashion and the secretion of TCTP from HEK293 cells increased when they over-expressed H(+)/K(+)-ATPase. In a murine model of ovalbumin-induced allergy, pretreatment with pantoprazole reduced infiltration of inflammatory cells, increased goblet cells, and increased TCTP secretion induced by OVA challenge. CONCLUSION:Since Omeprazole and pantoprazole decrease the secretion of TCTP which is associated with the development of allergic reaction, they may have the potential to serve as anti-allergic (asthmatic) drugs

Pleiotropic effect of the proton pump inhibitor esomeprazole leading to suppression of lung inflammation and fibrosis

Background: The beneficial outcome associated with the use of proton pump inhibitors (PPIs) in idiopathic pulmonary fibrosis (IPF) has been reported in retrospective studies. To date, no prospective study has been conducted to confirm these outcomes. In addition, the potential mechanism by which PPIs improve measures of lung function and/or transplant-free survival in IPF has not been elucidated. Methods: Here, we used biochemical, cell biological and preclinical studies to evaluate regulation of markers associated with inflammation and fibrosis. In our in vitro studies, we exposed primary lung fibroblasts, epithelial and endothelial cells to ionizing radiation or bleomycin; stimuli typically used to induce inflammation and fibrosis. In addition, we cultured lung fibroblasts from IPF patients and studied the effect of esomeprazole on collagen release. Our preclinical study tested efficacy of esomeprazole in a rat model of bleomycin-induced lung injury. Furthermore, we performed retrospective analysis of interstitial lung disease (ILD) databases to examine the effect of PPIs on transplant-free survival. Results: The cell culture studies revealed that esomeprazole controls inflammation by suppressing the expression of pro-inflammatory molecules including vascular cell adhesion molecule-1, inducible nitric oxide synthase, tumor necrosis factor-alpha (TNF-alpha) and interleukins (IL-1 beta and IL-6). The antioxidant effect is associated with strong induction of the stress-inducible cytoprotective protein heme oxygenase-1 (HO1) and the antifibrotic effect is associated with potent inhibition of fibroblast proliferation as well as downregulation of profibrotic proteins including receptors for transforming growth factor beta (TGF beta), fibronectin and matrix metalloproteinases (MMPs). Furthermore, esomeprazole showed robust effect in mitigating the inflammatory and fibrotic responses in a murine model of acute lung injury. Finally, retrospective analysis of two ILD databases was performed to assess the effect of PPIs on transplant-free survival in IPF patients. Intriguingly, this data demonstrated that IPF patients on PPIs had prolonged survival over controls (median survival of 3.4 vs 2 years). Conclusions: Overall, these data indicate the possibility that PPIs may have protective function in IPF by directly modulating the disease process and suggest that they may have other clinical utility in the treatment of extra-intestinal diseases characterized by inflammatory and/or fibrotic phases.Stanford School of Medicine [1049528-149- KAVFB]; Tobacco-Related Disease Research Program of the University of California [20FT-0090]; National Institutes of Health National Heart, Lung, and Blood Institute [5K01HL118683, P01HL114470]; Houston Methodist Research Institute [25150001]; Stanford SPARK Translational Research ProgramSCI(E)[email protected]