Trace and heavy metals analysis of Phyllanthus amarus and Phyllanthus fraternus in Ghana

Ethno-pharmacological uses and information about Phyllanthus species have prompted this work. The aim was to investigate the presence and levels of aluminum (Al), magnesium (Mg), iron (Fe), manganese (Mn) lead (Pb), cadmium (Cd), copper (Cu), zinc (Zn), chromium (Cr) and nickel (Ni) in Phyllanthus amarus (PA) and Phyllanthus fraternus (PF) in Ghana. Three different extracts (hot aqueous, hot ethanol and cold ethanol) were prepared from dried powdered samples of these plants. These extracts, including the dried samples of the plants were analyzed for the presence and levels of Al, Mg, Fe, Mn, Pb, Cd, Cu, Zn, Cr, and Ni using Atomic Absorption Spectrophotometry (AAS). The levels of Ni (0.43±0.24 ppm), Cr (0.35±0.04 ppm) and Cd (0.18±0.10 ppm) in both plant species were found to be very low in the dried samples plants and below the FAO/WHO maximum limits for vegetables, but were below the detectable limits of our AAS in the extract. Pb was not detected in the dried plant samples and in the extracts of the PA and PF; hence the plants may not pose serious health threat to consumers. PA and PF contain appreciable amounts of trace metals though they were all below the FAO/WHO maximum permissible limits in vegetables. The level of Fe in PA and PF (145.11±11.69 ppm and 179.94±14.60 ppm respectively) was found be to relatively high compared to the other elements analyzed. This finding makes the two plants suitable candidates for use in formulating effective remedies against iron deficiency diseases besides conferring some nutritive value to the patients.Keywords: Ethno-pharmacological, phytoextration, concentration, medicinal valu

Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology

Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p lt 0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p lt 0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum

Anti-Typhoid Properties of Phyllanthus Amarus Extracts

Phyllanthus amarus is a medicinal plant belonging to the family Euphorbiaceae and commonly known as ‘carry-me-seed’ or quinine weed. The whole plant was subjected to solvent extractions using petroleum ether and ethanol. Both crude extracts were tested for antimicrobial activity against Salmonella typhi using agar well-diffusion method of sensitivity testing. The crude etha-nolic extract showed good inhibitory effect against the bacteria but the petroleum ether extract showed no activity. The crude ethanol extract was subjected to column chromatographic separa-tion using dichloromethane: ethyl acetate (DCM/EA) solvent system. The column was finally eluted with methanol. The fractions eluted from the column were tested against the Salmonella typhi. The organism was sensitive to the methanol fractions at different concentrations (4.37mg/ml, 8.75mg/ml, 17.50mg/ml, 35.00mg/ml and 70.00mg/ml) with a zone of inhibition of 8mm, 12mm, 16mm, 20mm, and 22mm respectively. The Salmonella typhi was insensitive to the DCM/EA fractions. Phytochemical screening tests performed on the crude ethanolic extract revealed the presence of alkaloids, steroids, saponins, lignans, tannins and flavonoids