Evaluatıon Of The Regeneratıve Capacıty Of Trıphasıc Prototype Membranes Constructed For The Perıodontal Tıssue Engıneerıng Applıances In Vıtro

Son yıllarda gelişen periodontal doku mühendisliği yaklaşımı ile yönlendirilmiş doku rejenerasyonu prensipleri birleştirilerek yeni bir prototip membran tasarlandı ve üretildi. Bu çalışmanın hedefi polikaprolakton (PCL), nano hidroksiapatit (nHA), kollajen (COL) ve rhBMP-7 içeren ve hiyerarşik olarak görev görmesi planlanan çok katlı membranın MC3T3-E1 (fare osteojenik) hücrelerinde etkisini araştırmak, nHA nın kemik rejenerasyonuna katkısını ve rhBMP-7 yüklü membranın salım kinetiklerini ve hücre fonksiyonlarını değerlendirmektir. Membranların fiziksel özelliklerini incelemek amacıyla SEM, EDX, FTIR-ATR ve salım çalışmaları gerçekleştirildi. Çalışmalar sonucunda nHA ve kollajenin yapıya katıldığı ve çok katlı membranın SEM görüntülerinde çekirdek tabakasının por genişliğinin osteoblastlar için uygun büyüklükte olduğu (337 nm) bulundu. rhBMP-7 içeren membrandaki rhBMP-7 nin %42 sini ilk 4 saat içinde, kalanının ise 3 gün boyunca salım gösterdiği bulgulandı. Yapılan in vitro çalışmadan elde edilen SEM görüntülerinde ise, membranın dış tabakasına hücrelerin tutunduğu, yer yer içerisine girdiği ve ekstrasellüler matriksi oluşturduğu bulgulandı. Tüm membranlarda hücrelerin canlı olduğu ve en çok rhBMP-7 varlığında çoğalma olduğu MTT analizi ile gözlendi. Hücrelerin osteoblastik farklılaşmasını gösteren belirteçler ALP analizi ile, osteojenik gen sonuçları gerçek zamanlı RT-qPCR ile belirlendi. rhBMP-7 li grupta ALP aktivitesi ve COL-1, Runx2, OCN, OPN gen düzeyleri anlamlı olarak yüksek bulundu. Bu çalışma üç farklı içerikte hazırlanmış membranlardan rhBMP-7 ile yüklenmiş PCL, nHA, COL içeren üç fazlı prototip membranın periodontal doku rejenerasyonda etkin bir membran olabileceğini göstermiştir.A novel prototype membrane has been designed and fabricated with a graded structure (polycaprolactone- PCL, collagen- COL, nanohydroxyapatite- nHA) by combining recently emerging periodontal tissue engineering field with guided tissue regeneration principles. The purpose of thesis study is to research effect of the triphasic membranes, which are planned to serve hierarchically on MC3T3-E1 cells, to evaluate nHA for bone regeneration and to release kinetics of the membrane loaded with rhBMP-7. SEM, EDX, FTIR-ATR and release studies of the membranes which defines membranes physical properties have been performed. As a result of these studies, it has been determined nHA and collagen joined the membrane successfully, the core layer had a suitable size for osteoblasts (337 nm). It was also discovered that 42% of rhBMP-7 was released during the first 4 hours and the remainder was released during the next three days. In SEM images, cells adhere to even at same places penetrate into the inner layer of the membrane and ECM formed. It was observed through MTT analysis that vitality of the cells within the membranes as well as greatest rate of proliferation occured in presence of rhBMP-7. Osteoblastic differentiation markers have been determined through ALP analysis and osteogenic gene results through real time RT-qPCR. ALP activity COL-I, Runx2, OCN, OPN gene levels have been found significantly higher in BMP-7 group. In conclusion, the data we obtained in three different prototype membranes, the membrane laden with rhBMP-7 and containing PCL, nHA, COL can be an effective membrane in periodontal tissue regeneration

Effects of interactive power toothbrush with or without application assistance on the plaque, gingivitis, and gingival abrasion among dental students: a randomized controlled clinical trial

Objective The purpose of this randomized controlled study is to determine if an interactive power toothbrush (PTB) with the application (app) promotes additional benefits on dental plaque, gingivitis, and gingival abrasion to that of a manual toothbrush (MTB).Materials and methods Ninety participants (dental students) diagnosed with gingivitis were randomly assigned, into threegroups, 1:1:1 ratio, given an app-assisted interactive PTB (PTB-A) (Oral-B Genius® Rechargeable 8000 PTB with CrossAction brush head) or the same PTB without app-assistance (PTB-NA) or a regular MTB for home-use. Plaque was assessedusing the Turesky modification of the Quigley and Hein Plaque Index (QHPI-TM), and gingivitis was evaluated using theBleeding on Marginal Probing Index (BOMP) at baseline (T0), week 2 (T1), and week 8 (T2). Gingival abrasions wererecorded at T1 and T2. An exit survey was used to assess brushing time and visual-analog-scale (VAS) scores.Results PTB improved QHPI-TM significantly in terms of time-dependent changes, irrespective of app assistance. BOMPwas observed to be statistically lower in PTB-A than in MTB only at T2. Brushing times were longer in PTB-A than inMTB. Gingival abrasion was less in both PTB and MTB. PTB-A had better results than MTB in cleaning ability accordingto subject-reported outcomes.Conclusions Both PTBs were more effective than MTB; however, the app-assisted interactive PTB was similar to the nonassisted one in plaque removal efficacy, gingivitis reduction, and incidence of gingival abrasion, yet better than MTB incleaning ability.</p

Evaluation of Mid-buccal Gingival Recessions and Occlusal Interferences

Background: Dental malocclusions may cause disruption in occlusal harmony, and de-structive interferences during mandibular functional movements can be seen. Ideal occlusal contacts during dynamic mandibular movements may be crucial for prevent-ing the occurrence of mid- buccal gingival recession (mbGR). While determining mbGR risk factors in young adults, the effect of occlusal interferences on mbGR has not yet been focused on. Based on this gap, this field needs to be clarified with new studies.Objective: The aim of this case–control study was to evaluate the relationships be-tween the presence, extent and severity of mbGRs to dental malocclusions, occlusal interferences in anterior (AG) and lateral guidance (LG) and to determine the potential risk indicators in a young population.Methods: A total of 149 dental students were comprised and 70 of them presented mbGR(s) and 79 did not (18–25 years, 4553 teeth). Periodontal status was assessed with full mouth bleeding (FMBS) and plaque score (FMPS), probing depth, clinical at-tachment level, recession depth and keratinised tissue width (KTW) by a periodontist. Malocclusions and occlusal interferences were evaluated by an orthodontist. Logistic regression analyses provided data on the effect of occlusal interferences and the other indicators towards mbGR.Results: The mean of the number of teeth with mbGR(s) per subject was 4.3. The mean of the overall extent of teeth with mbGR(s) was 14.2%. FMBS, decreased KTW, self-reported bruxism, group function occlusion, increased contact number of all teeth and only premolars/molars in AG or LG and Class III malocclusions were signifi-cantly associated with the presence of mbGR. Decreased KTW presenting mbGR in the mandible and non-carious cervical lesion adjunct to mbGR significantly increased the odds of the severity of mbGR. Group function occlusion revealed higher mbGRs in premolar/molars than canine guided occlusion.Conclusion: Increase in the occlusal interferences in premolars/molars during lateral and anterior guidance may have an effect on the presence and severity of mbGR. Further studies should be designed to confirm these findings.</p