39 research outputs found
Molecular identification of HIV-1 in the presence of hepatitis B virus and hepatitis C virus Co-infections
Bu çalışma, 14-17 Eylül 2016 tarihleri arasında Lizbon[Portekiz]'de düzenlenen 19. Meeting of the European Society for Clinical Virology'de bildiri olarak sunulmuştur.Background: Because of their similar modes of transmission, the simultaneous infection of viral hepatitis and human immunodeficiency virus are increasingly seen as a big problem related to human health.
Aims: To determine the drug mutations in hepatitis B virus and/or hepatitis C virus co-infected human immunodeficiency virus-1 patients in Turkey.
Study Design: Retrospective cross-sectional study.
Methods: The present study was conducted between 2010 and 2017. HBsAg, anti-hepatitis C virus, and anti-human immunodeficiency vim were tested with ELISA. All anti-human immunodeficiency virus positive results by ELISA were verified for anti-human immunodeficiency virus positivity by a Western blot test, and Antihuman immunodeficiency virus positive patients with HBsAg andior anti-hepatitis C virus positivity were included in the study. Subtyping and genotypic resistance analyses were performed by population sequencing of the viral protease and reverse transcriptase regions of the human immunodeficiency virus-1 pol gene.
Results: We detected 3896 human immunodeficiency virus-1 positive patients whose sera were sent from numerous hospitals across the country to our polymerase chain reaction unit for detection of drug resistance mutations and whose molecular laboratory tests were completed. Viral hepatitis co-infections were detected in 4.3% (n=170) of patients. Hepatitis B virus and hepatitis C virus co-infection were observed in 3.2% and 0.5% of all human immunodeficiency virus-I infected patients, respectively. The major human immunodeficiency virus-1 subtype detected was group M, subtype B (62.9%). However, 13.5% of drug resistance mutation motifs were found in human immunodeficiency virus-1 genomes of patients included in the study.
Conclusion: Due to similar transmission routes, HIV1 patients are at risk of hepatitis B and C virus co-infection. However, antiretroviral drug resistance mutation model is similar to patients with hepatitis negative.European Society for Clinical Virolog
Ni'meti divanı
TEZ6168Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 2007.Kaynakça (s.270-272) var.viii, 350 s. ; 29 cm.Bu çalışmada 17. yüzyıl şairlerinden olan Ni'metî'nin Divanı'nın çeviri yazılı metni hazırlanmış; divanın biçim, dil ve üslûp, içerik incelemesi yapılmış; şairin hayatı, edebî kişiliği, eserleri hakkında bilgi verilmiştir. Ni'metî, döneminin şairlerinden farklı olarak sade bir dil kullanmış, Divan Edebiyatının gelenekselleşmiş konularıyla birlikte, toplumsal eleştiri nitelikli şiirler yazmıştır. Divanlarda pek görülmeyen bahr-i tavil, manzum mektup gibi farklı şekilleri denemiştir. ªairin 17. yüzyılın sosyal yapısından etkilendiği ve bunu divanına yansıttığı görülmektedir. Türk Edebiyatında fazla tanınmamış olan Ni'metî'nin ve eserinin bu çalışmayla incelenmesine gayret edilmiştir.In this study, the translated text of the divan of Ni'metî, who is one of the Seventeenth century poets, is prepared; a study of divan's form,language and style, content is made; information on the poet's life, his literary identity and his works is given. Ni'metî, unlike to the poets of the period, used an austere language; together with the traditional topics of Divan Literature he wrote poems which carry a characteristic of social criticism. Bahr-i tavil, which isn't often seen in divans, tried different forms such as letter in verse. It seems that the poet was influenced by the social structure of the 17th century and that he reflected this on his divan. It was tried hard to examine Ni'metî, who isn't known much in Turkish Literature, and his work by means of this study.Bu çalışma Ç.Ü. Bilimsel Araştırma Projeleri Birimi Tarafından Desteklenmiştir. Proje No
Resistance to Penicillin and Erythromycin of 28 Streptococcus pneumoniae Strains, Isolated from Sterile Body Sites
Resistance of 28 Streptococcus pneumoniae strains isolated from sterile body sites to penicillin and erythromycin was studied with disk diffusion and E test methods. Intermediate penicillin resistance was found in 4 (%14.3) strains and erythromycin resistance was found in 2 strains (%7). High level penicillin-resistance was not detected
The Importance of Microbiological Diagnosis of Gas Gangrene
The direct Gram staining examinations of materials in gas gangrene quickly provide valuable information for the clinician in establishing the diagnosis when a rapid decision about therapy is necessary to be made. Here the importance of laboratory diagnosis of gas gangrene has been desired to be emphasized on the occasion of two materials examined in our laboratory at different times. Both materials were obtained in the operating room before surgery. Gram-stained smears were quickly examined and the operating room was informed at once that the microscopic apperances were well-proposed to Clostridium and supported the diagnosis of gas gangrene. Anaerobic jar was used for the anaerobic cultures. C. septicum and C. perfringens were biochemically identified in anaerobic culture of the first material. But mixed colonies were produced in the anaerobic culture of the second material and further identification of the second one could not be done because of inability to keep them alive. Consequently it has been emphasized that in a severe infection such as gas gangrene a simple gram staining provides a useful information for the physician to choose a treatment and the most common isolates can be identified by some simple tests which are able to be done in all laboratory conditions
Total Antioxidant Capacity in Patients with Chronic Viral Hepatitis
Total antioxidant capacity (TAOC) was investigated in 38 chronic hepatitis patients (25 hepatitis B, 13 hepatitis C). Control group included 22 healthy persons. Results of the colorimetric measurements of TAOC with Randox kit showed that TAOC was significantly lower in patients with chronic hepatitis compared to the controls (p= 0.0013). The findings of this study and those of some related studies, which are limited in number, suggest that antioxidant treatment may contribute to current treatments
The Investigation of Seroprevalance of Brucellosis and Related Factors with A Questionnaire in Mersin Province
Brucellosis seropositivity was searched using serum agglutination test on some selected groups in Mersin province between October and November 2003. In this study, Brucella abortus antigen provided from Pendik Veterinarian Research Institute was used. Sera with titers equal or more than 1/10 were accepted as positive. A questionnaire was applied to the subjects to reveal sociodemographic properties, animal occupation, consumption of milk and milk products and complaints about brucellosis. In a population of 600 persons, the seroprevalence rate of brucellosis was found as 13.2%. The relationships between agglutination test positivity and educational background, settlement place, occupation, animal breeding, consumption of milk and milk products, and complaints of loss of appetite and weight loss were found significant
An assessment of health and safety practices for employees at health care institutions within the city of Mersin
Objective: The aim of this research was to assess employee health and safety practices, in public and private healthcare institutions providing services in the city of Mersin. Methods: The data of this descriptive study was collected through questionnaires conducted in September 2015. Thirty-four healthcare institutions in total were included for this work. These institutions have been providing second and third tier health services for the city center, as well as the surrounding districts of Mersin. Results: When assessed in terms of the number of employees in occupational health and work safety services, only 10.1% of health workers were found to have satisfactory access to occupational physicians, whereas only 0.8% received enough support from occupational health and safety experts. When the access to both the occupational physicians and the occupational health and safety experts were evaluated together, only 0.8% of the health workers were found to be able to receive adequate contact time. In 2014, 52.9% of the employees were examined periodically and 94.1% of the institutions had established risk assessments. Conclusion: In Mersin, only 1% of the health workers are provided with occupational health and work safety services for a sufficient period of time by occupational physicians and occupational health and safety experts. This has been highlighted as a major shortcoming.</p
Antimicrobial Resistances of Escherichia coli Isolates Causing Bacteremia (2004-2009)
Introduction: Escherichia coli is the most frequently isolated microorganism causing nosocomial and community-onset bacteremia. The aim of the present study was to investigate antimicrobial resistance trends of E. coli isolates causing bacteremia. Materials and Methods: In this retrospective study conducted at the University of Mersin, antimicrobial resistances were evaluated in a total of 323 E. coli isolates causing bacteremia between January 2004 and January 2010. All E. coli species were isolated from the blood using the BACTEC 9240 system (Becton Dickinson, INC, Sparks, MD). Isolates were identified and antimicrobial susceptibility testing was performed using standard techniques. Extended-spectrum beta-lactamase production was assessed by the double-disk synergy test. Nosocomial bacteremia was defined as an infection not present or incubating at the time of hospital admission, with onset at least 48 hours after admission to the hospital. Antimicrobial resistance trends of E. coli isolates during two three-year periods (2004- 2006 and 2007-2009) were prospectively recorded, evaluated and compared. SPSS ver. 16 (Chicago, IL) statistical package program was used for statistical analysis. Results: Patients were analyzed; 58.2% were male, the mean age was 52.7 ± 24.7 (1-96) years, and duration of bacteremia was 7.9 ± 4.6 (1-23). Of these patients, 92.3% had nosocomial bacteremia due to E. coli. Overall mortality was 27.2%. Extended-spectrum beta-lactamase-producing strains were reported in 136 (42.1%) cases. During the six-year study period, resistance rates of E. coli isolates causing bacteremia increased from 42.6% to 67.9% for cefuroxime axetil (p=0.002), from 42.3% to 62.3% for trimethoprimsulfamethoxazole (p=0.003), from 37% to 57.9% for ciprofloxacin (p= 0.018), and from 29.6% to 53.6% for gentamicin (p=0.004). Resistance rates to imipenem remained very low and stable (from 1.1% to 1.9%). Quinolone resistance was significantly associated with extended- spectrum beta-lactamase-production (p=0.0001). Conclusion: We demonstrated a trend of increasing resistance among E. coli isolates causing bacteremia to four different classes of antimicrobials. Increasing resistance may have an impact on the choice of empirical antimicrobial therapy in patients with bacteremia