Contamination par le virus de l'hépatite C lors de scléroses des varices des membres inférieurs (enquête sur une épidémie)

BORDEAUX2-BU Santé (330632101) / SudocPARIS-BIUM (751062103) / SudocPARIS-Bib. Serv.Santé Armées (751055204) / SudocSudocFranceF

Progenitor “Mycobacterium canettii” Clone Responsible for Lymph Node Tuberculosis Epidemic, Djibouti

“Mycobacterium canettii,” an opportunistic human pathogen living in an unknown environmental reservoir, is the progenitor species from which Mycobacterium tuberculosis emerged. Since its discovery in 1969, most of the ≈70 known M. canettii strains were isolated in the Republic of Djibouti, frequently from expatriate children and adults. We show here, by whole-genome sequencing, that most strains collected from February 2010 through March 2013, and associated with 2 outbreaks of lymph node tuberculosis in children, belong to a unique epidemic clone within M. canettii. Evolution of this clone, which has been recovered regularly since 1983, may mimic the birth of M. tuberculosis. Thus, recognizing this organism and identifying its reservoir are clinically important

Significance of the Identification in the Horn of Africa of an Exceptionally Deep Branching <em>Mycobacterium tuberculosis</em> Clade

<div><p>Molecular and phylogeographic studies have led to the definition within the <em>Mycobacterium tuberculosis</em> complex (MTBC) of a number of geotypes and ecotypes showing a preferential geographic location or host preference. The MTBC is thought to have emerged in Africa, most likely the Horn of Africa, and to have spread worldwide with human migrations. Under this assumption, there is a possibility that unknown deep branching lineages are present in this region. We genotyped by spoligotyping and multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) 435 MTBC isolates recovered from patients. Four hundred and eleven isolates were collected in the Republic of Djibouti over a 12 year period, with the other 24 isolates originating from neighbouring countries. All major <em>M. tuberculosis</em> lineages were identified, with only two <em>M. africanum</em> and one <em>M. bovis</em> isolates. Upon comparison with typing data of worldwide origin we observed that several isolates showed clustering characteristics compatible with new deep branching. Whole genome sequencing (WGS) of seven isolates and comparison with available WGS data from 38 genomes distributed in the different lineages confirms the identification of ancestral nodes for several clades and most importantly of one new lineage, here referred to as lineage 7. Investigation of specific deletions confirms the novelty of this lineage, and analysis of its precise phylogenetic position indicates that the other three superlineages constituting the MTBC emerged independently but within a relatively short timeframe from the Horn of Africa. The availability of such strains compared to the predominant lineages and sharing very ancient ancestry will open new avenues for identifying some of the genetic factors responsible for the success of the modern lineages. Additional deep branching lineages may be readily and efficiently identified by large-scale MLVA screening of isolates from sub-Saharan African countries followed by WGS analysis of a few selected isolates.</p> </div

comparison of the Percy556 <i>mce3</i> deletion 79 with the organisation of the operon in H37Rv, in “<i>M. canettii”</i> strain CIPT140010059, and in <i>M. bovis</i> BCG.

<p>Four different organizations are observed. The most parsimonious explanation is that the <i>M. canettii</i> configuration represents the ancestral situation from which 3 different deletions are derived via at least 2 independent deletion events.</p

Minimum spanning tree based upon whole genome SNP analysis.

<p>The tree is based upon 13382 SNPs. The tree size is 13463, i.e. it contains approximately 0.6% of homoplasia. The length of each branch expressed in SNP numbers is indicated. The red star marks the approximate branching point of the <i>M. canettii</i> lineage according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052841#pone.0052841-Namouchi1" target="_blank">[19]</a>. The two blue stars indicate the positions of newly defined ancestral nodes within the two Africanum lineages 5 and 6.</p

Minimum spanning tree representation of the clustering of 435 isolates from the Horn of Africa.

<p>The color code reflects the main MLVA clusters. Lineages (1 to 6) and some sublineages (CDC1551, H37Rv, …) are indicated. The size of the circles reflects the number of isolates with an identical genotype. Branches longer than 10 are not drawn. The main outlier candidates are arrowed (red arrows: isolates selected for sequencing).</p

Minimum spanning tree representation of the Horn of Africa isolates with respect to 700 isolates of various origins.

<p>The 435 isolates from the Horn of Africa are displayed in white, whereas the isolates of worldwide origins are colored according to lineage using the same color code as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052841#pone-0052841-g001" target="_blank">Figure 1</a>. Red arrows: isolates selected for sequencing. The minimum spanning tree analysis is based upon the 19 VNTR loci shared by the 24 loci MLVA assays used by <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052841#pone.0052841-Fabre2" target="_blank">[13]</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052841#pone.0052841-Supply1" target="_blank">[22]</a>.</p

Schematic representation of the main historic events along the lineage 7 and Percy256-Percy556 associated geotype.

<p>The evolution of lineage 7 is displayed in a linear fashion from the <i>M. tuberculosis</i> ancestor (the obligate human pathogen in contrast to its environmental unknown <i>Mycobacteria</i> progenitor) to the Percy256-Percy556 geotype representative. The relative timing of the different splits is indicated. The hypothetical temporal succession of the split of the two Ancestral superlineages indicated here is suggested by the slightly abnormal mutation pattern along branch (6,7). More precise rooting of the MTBC will be needed to test this hypothesis.</p