msqrob2PTM: differential abundance and differential usage analysis of MS-based proteomics data at the post-translational modification and peptidoform level

Abstract In the era of open-modification search engines, more post-translational modifications than ever can be detected by LC-MS/MS-based proteomics. This development can switch proteomics research into a higher gear, as PTMs are key in many cellular pathways important in cell proliferation, migration, metastasis and ageing. However, despite these advances in modification identification, statistical methods for PTM-level quantification and differential analysis have yet to catch up. This absence can partly be explained by the inherently low abundance of many PTMs and the confounding of PTM intensities with its parent protein abundance. Therefore, we have developed msqrob2PTM, a new workflow in the msqrob2 universe capable of differential abundance analysis at the PTM, and at the peptidoform level. The latter is important for validating PTMs found as significantly differential. Indeed, as our method can deal with multiple PTMs per peptidoform, there is a possibility that significant PTMs stem from one significant peptidoform carrying another PTM, hinting that it might be the other PTM driving the perceived differential abundance. Our workflows can flag both Differential Peptidoform (PTM) Abundance (DPA) and Differential Peptidoform (PTM) Usage (DPU). This enables a distinction between direct assessment of differential abundance of peptidoforms (DPA) and differences in the relative usage of peptidoforms corrected for corresponding protein abundances (DPU). For DPA, we directly model the log2-transformed peptidoform (PTM) intensities, while for DPU, we correct for parent protein abundance by an intermediate normalisation step which calculates the log2-ratio of the peptidoform (PTM) intensities to their summarized parent protein intensities. We demonstrated the utility and performance of msqrob2PTM by applying it to datasets with known ground truth, as well as to biological PTM-rich datasets. Our results show that msqrob2PTM is on par with, or surpassing the performance of, the current state-of-the-art methods. Moreover, msqrob2PTM is currently unique in providing output at the peptidoform level

Fluids in the South Armorican Shear Zone, France

International audienceThe South Armorican Shear Zone (SASZ) is alithsopheric-scale shear zone from western Europethat was active during Variscan times. U–Pb analyseson zircon and monazite and 40Ar-39Ar analyses onmuscovite from mylonites and syntectonic granitesdefine a minimum duration of 20 Ma for thedeformation and magmato-hydrothermal historyalong the SASZ, between 320 Ma and 300 Ma. Giantquartz veins associated with the deformed zonesrecord important crustal-scale fluid circulations. Mostquartz veins have δ18O values between 10 and 16‰,indicating a crustal origin for the fluids.Microthermometry on fluid inclusions from euhedralquartz indicates that late fluids were mostly aqueouswith very low salinity (0–1.7 wt% eq.) and withhomogenization temperatures ranging between 150and 270 °C. Together with very low δ18O values ofsome euhedral quartz down to -2‰, these featuresargue for a surface origin for these fluids. Calculatedδ18Ofluid values of about -11‰ reflect surface-derivedfluids sourced at high elevation. Independantly, somemylonites from the SASZ have low δ18O silicatevalues compared to their undeformed protolith, whichhas to be related to the influx of surface-derivedwaters. The heat source necessary for this crustalscale downward fluid infiltration followed by upwardmotion was likely provided by the exhumation oflower crustal units in the South Armorican domain.New investigations are currently in progress, notablyδD measurements on micas from mylonites from boththe SASZ and associated detachments to betterconstrain the paleo-altitude reached during theorogeny. Various ore deposits were formed duringthese events, so that this integrated study should helpto draw the metallogenic crustal landscape of thisregio