N-(2,6-Dimethyl-3-oxo-1-thia-4-aza­spiro­[4.5]dec-4-yl)-2-hydr­oxy-2,2-di­phenyl­acetamide

In the title compound, C24H28N2O3S, the pendant methyl C atom bonded to the cyclo­hexane ring is disordered over two sites in a 0.580 (11):0.420 (11) ratio. The cyclo­hexane ring adopts a distorted chair conformation while the thia­zolidine ring has an envelope conformation. The two phenyl rings make a dihedral angle of 71.8 (2)° with each other. The conformation is stabilized by an intra­molecular N—H⋯O hydrogen bond. In the crystal structure, an inter­molecular hydrogen bond O—H⋯O occurs

Development of Thiazolidinones as Fungal Carbonic Anhydrase Inhibitors

In our efforts to find new and selective thiazolidinone-based anti-Candida agents, we synthesized and tested 26 thiazolidinones against several Candida spp. and Gram-positive and Gram-negative bacteria. The compounds showed selective antifungal activity with potency similar to fluconazole and clotrimazole, while lacking strong antibacterial activity. Molecular docking and molecular dynamics studies were performed on Candida CYP51a1 and carbonic anhydrase (CA) enzymes to further suggest putative targets that could mediate the antifungal effects of these compounds. Finally, the compounds were tested in enzyme inhibition assays to assess their putative mechanism of action and showed promising KI values in the 0.1–10 µM range against the Candida glabrata β-CA enzyme CgNce103